Label-free imaging of immune cell dynamics in the living retina using adaptive optics
Our recent work characterized the movement of single blood cells within the retinal vasculature (Joseph et al. 2019) using adaptive optics ophthalmoscopy. Here, we apply this technique to the context of acute inflammation and discover both infiltrating and tissue-resident immune cells to be visible...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2020-10-01
|
| Series: | eLife |
| Subjects: | |
| Online Access: | https://elifesciences.org/articles/60547 |
| _version_ | 1811199749693374464 |
|---|---|
| author | Aby Joseph Colin J Chu Guanping Feng Kosha Dholakia Jesse Schallek |
| author_facet | Aby Joseph Colin J Chu Guanping Feng Kosha Dholakia Jesse Schallek |
| author_sort | Aby Joseph |
| collection | DOAJ |
| description | Our recent work characterized the movement of single blood cells within the retinal vasculature (Joseph et al. 2019) using adaptive optics ophthalmoscopy. Here, we apply this technique to the context of acute inflammation and discover both infiltrating and tissue-resident immune cells to be visible without any labeling in the living mouse retina using near-infrared light alone. Intravital imaging of immune cells can be negatively impacted by surgical manipulation, exogenous dyes, transgenic manipulation and phototoxicity. These confounds are now overcome, using phase contrast and time-lapse videography to reveal the dynamic behavior of myeloid cells as they interact, extravasate and survey the mouse retina. Cellular motility and differential vascular responses were measured noninvasively and in vivo across hours to months at the same retinal location, from initiation to the resolution of inflammation. As comparable systems are already available for clinical research, this approach could be readily translated to human application. |
| first_indexed | 2024-04-12T01:52:32Z |
| format | Article |
| id | doaj.art-49ea3b19d11d461880607fa1c332efda |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-12T01:52:32Z |
| publishDate | 2020-10-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-49ea3b19d11d461880607fa1c332efda2022-12-22T03:52:53ZengeLife Sciences Publications LtdeLife2050-084X2020-10-01910.7554/eLife.60547Label-free imaging of immune cell dynamics in the living retina using adaptive opticsAby Joseph0https://orcid.org/0000-0001-8143-801XColin J Chu1https://orcid.org/0000-0003-2088-8310Guanping Feng2Kosha Dholakia3Jesse Schallek4https://orcid.org/0000-0002-6337-4187The Institute of Optics, University of Rochester, Rochester, United StatesTranslational Health Sciences, University of Bristol, Bristol, United KingdomDepartment of Biomedical Engineering, University of Rochester, Rochester, United StatesFlaum Eye Institute, University of Rochester, Rochester, United StatesFlaum Eye Institute, University of Rochester, Rochester, United States; Department of Neuroscience and the Del Monte Institute for Neuroscience, University of Rochester, Rochester, United States; Center for Visual Science, University of Rochester, Rochester, United StatesOur recent work characterized the movement of single blood cells within the retinal vasculature (Joseph et al. 2019) using adaptive optics ophthalmoscopy. Here, we apply this technique to the context of acute inflammation and discover both infiltrating and tissue-resident immune cells to be visible without any labeling in the living mouse retina using near-infrared light alone. Intravital imaging of immune cells can be negatively impacted by surgical manipulation, exogenous dyes, transgenic manipulation and phototoxicity. These confounds are now overcome, using phase contrast and time-lapse videography to reveal the dynamic behavior of myeloid cells as they interact, extravasate and survey the mouse retina. Cellular motility and differential vascular responses were measured noninvasively and in vivo across hours to months at the same retinal location, from initiation to the resolution of inflammation. As comparable systems are already available for clinical research, this approach could be readily translated to human application.https://elifesciences.org/articles/60547adaptive opticsretinalabel-free imagingintravitalimmune response, inflammationimmune cell motility |
| spellingShingle | Aby Joseph Colin J Chu Guanping Feng Kosha Dholakia Jesse Schallek Label-free imaging of immune cell dynamics in the living retina using adaptive optics eLife adaptive optics retina label-free imaging intravital immune response, inflammation immune cell motility |
| title | Label-free imaging of immune cell dynamics in the living retina using adaptive optics |
| title_full | Label-free imaging of immune cell dynamics in the living retina using adaptive optics |
| title_fullStr | Label-free imaging of immune cell dynamics in the living retina using adaptive optics |
| title_full_unstemmed | Label-free imaging of immune cell dynamics in the living retina using adaptive optics |
| title_short | Label-free imaging of immune cell dynamics in the living retina using adaptive optics |
| title_sort | label free imaging of immune cell dynamics in the living retina using adaptive optics |
| topic | adaptive optics retina label-free imaging intravital immune response, inflammation immune cell motility |
| url | https://elifesciences.org/articles/60547 |
| work_keys_str_mv | AT abyjoseph labelfreeimagingofimmunecelldynamicsinthelivingretinausingadaptiveoptics AT colinjchu labelfreeimagingofimmunecelldynamicsinthelivingretinausingadaptiveoptics AT guanpingfeng labelfreeimagingofimmunecelldynamicsinthelivingretinausingadaptiveoptics AT koshadholakia labelfreeimagingofimmunecelldynamicsinthelivingretinausingadaptiveoptics AT jesseschallek labelfreeimagingofimmunecelldynamicsinthelivingretinausingadaptiveoptics |