Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)
Indonesia is an agrarian country whose territory is partly used as an agricultural sector, has especially watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai). At present, the development of watermelons in Indonesia still requires a variety of colors, superior watermelons need to be developed...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Gadjah Mada
2021-11-01
|
| Series: | Journal of Tropical Biodiversity and Biotechnology |
| Subjects: | |
| Online Access: | https://jurnal.ugm.ac.id/jtbb/article/view/61858 |
| _version_ | 1798035042540191744 |
|---|---|
| author | Andra Jausa Salsabila Budi Setiadi Daryono Aprilia Sufi Subiastuti |
| author_facet | Andra Jausa Salsabila Budi Setiadi Daryono Aprilia Sufi Subiastuti |
| author_sort | Andra Jausa Salsabila |
| collection | DOAJ |
| description | Indonesia is an agrarian country whose territory is partly used as an agricultural sector, has especially watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai). At present, the development of watermelons in Indonesia still requires a variety of colors, superior watermelons need to be developed by making triploid orange watermelons. The new watermelon cultivar (F₁) is the result of crossing 'Jelita' (♀) and 'BallyBall' (♂) which are expected to provide a combination of characters that can produce large and sweet fruits. The purpose of this study was to identify ISSR markers that were associated with ploidy levels of horticultural crops, particularly watermelon. The watermelons were growns on the agricultural land of Kebondalem Hamlet, Madurejo Village, Prambanan District, D.I.Y. and analyzed at the Laboratory of Biotechnology PAU, UGM and Laboratory of Genetics and Breeding, Faculty of Biology UGM. Analysis of genetic variation was carried out using the PCR-ISSR method and the DNA bandwidth was calculated using Paint Apps, Microsoft Excel 2013, and Microsoft Word 2013. The results of crossing 'Jelita' and 'King Quality 'is sterile. The result of 'Jelita' with 'BallyBall' produces tillers until sterile harvest time. The results of the analysis of genetic variation using the PCR-ISSR method indicate that the ISSR BI, B3, B5, and CBTC 1 molecular markers cannot be used to distinguish ploidy from the tested watermelons. |
| first_indexed | 2024-04-11T20:52:43Z |
| format | Article |
| id | doaj.art-49eef2de70aa40deac039b86971f5bb8 |
| institution | Directory Open Access Journal |
| issn | 2540-9573 2540-9581 |
| language | English |
| last_indexed | 2024-04-11T20:52:43Z |
| publishDate | 2021-11-01 |
| publisher | Universitas Gadjah Mada |
| record_format | Article |
| series | Journal of Tropical Biodiversity and Biotechnology |
| spelling | doaj.art-49eef2de70aa40deac039b86971f5bb82022-12-22T04:03:47ZengUniversitas Gadjah MadaJournal of Tropical Biodiversity and Biotechnology2540-95732540-95812021-11-016310.22146/jtbb.6185830776Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai)Andra Jausa Salsabila0Budi Setiadi Daryono1Aprilia Sufi SubiastutiFaculty of Biology, Universitas Gadjah MadaFaculty of Biology, Universitas Gadjah MadaIndonesia is an agrarian country whose territory is partly used as an agricultural sector, has especially watermelon (Citrullus lanatus (Thunb.) Matsum & Nakai). At present, the development of watermelons in Indonesia still requires a variety of colors, superior watermelons need to be developed by making triploid orange watermelons. The new watermelon cultivar (F₁) is the result of crossing 'Jelita' (♀) and 'BallyBall' (♂) which are expected to provide a combination of characters that can produce large and sweet fruits. The purpose of this study was to identify ISSR markers that were associated with ploidy levels of horticultural crops, particularly watermelon. The watermelons were growns on the agricultural land of Kebondalem Hamlet, Madurejo Village, Prambanan District, D.I.Y. and analyzed at the Laboratory of Biotechnology PAU, UGM and Laboratory of Genetics and Breeding, Faculty of Biology UGM. Analysis of genetic variation was carried out using the PCR-ISSR method and the DNA bandwidth was calculated using Paint Apps, Microsoft Excel 2013, and Microsoft Word 2013. The results of crossing 'Jelita' and 'King Quality 'is sterile. The result of 'Jelita' with 'BallyBall' produces tillers until sterile harvest time. The results of the analysis of genetic variation using the PCR-ISSR method indicate that the ISSR BI, B3, B5, and CBTC 1 molecular markers cannot be used to distinguish ploidy from the tested watermelons.https://jurnal.ugm.ac.id/jtbb/article/view/61858issr, marker assisted breeding, orange watermelon, ploidy, triploid |
| spellingShingle | Andra Jausa Salsabila Budi Setiadi Daryono Aprilia Sufi Subiastuti Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai) Journal of Tropical Biodiversity and Biotechnology issr, marker assisted breeding, orange watermelon, ploidy, triploid |
| title | Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai) |
| title_full | Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai) |
| title_fullStr | Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai) |
| title_full_unstemmed | Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai) |
| title_short | Identification of ISSR-based Molecular Markers Associated with Ploidy Level of Orange Watermelon (<i>Citrullus lanatus</i> (Thunb.) Matsum & Nakai) |
| title_sort | identification of issr based molecular markers associated with ploidy level of orange watermelon i citrullus lanatus i thunb matsum nakai |
| topic | issr, marker assisted breeding, orange watermelon, ploidy, triploid |
| url | https://jurnal.ugm.ac.id/jtbb/article/view/61858 |
| work_keys_str_mv | AT andrajausasalsabila identificationofissrbasedmolecularmarkersassociatedwithploidyleveloforangewatermelonicitrulluslanatusithunbmatsumnakai AT budisetiadidaryono identificationofissrbasedmolecularmarkersassociatedwithploidyleveloforangewatermelonicitrulluslanatusithunbmatsumnakai AT apriliasufisubiastuti identificationofissrbasedmolecularmarkersassociatedwithploidyleveloforangewatermelonicitrulluslanatusithunbmatsumnakai |