Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays
Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commerciali...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Genética
2008-01-01
|
| Series: | Genetics and Molecular Biology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100021 |
| _version_ | 1819080974511636480 |
|---|---|
| author | Marilanda Ferreira Bellini Leonardo Neves Cabrioti Ana Paula Terezan Berenice Quinzani Jordão Lúcia Regina Ribeiro Mário Sérgio Mantovani |
| author_facet | Marilanda Ferreira Bellini Leonardo Neves Cabrioti Ana Paula Terezan Berenice Quinzani Jordão Lúcia Regina Ribeiro Mário Sérgio Mantovani |
| author_sort | Marilanda Ferreira Bellini |
| collection | DOAJ |
| description | Functional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann), also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM). Different methanolic extract fractions (ME) of this mushroom were submitted to the cytokinesis-block micronucleus (CBMN) clastogenic assay and the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT) assay for gene mutation, both using Chinese hamster ovary cells clone K1 (CHO-K1). The results suggest that all the fractions tested possess cytotoxic and mutagenic potential but no clastogenic effects. Further information is needed on the biochemical components of the A. blazei methanol fractions to identify any substances with cytotoxic and/or mutagenicity potential. These findings indicate that A. blazei methanolic extract should not be used due to their genotoxicity and care should be taken in the use of A. blazei by the general population until further biochemical characterization of this fungi is completed. |
| first_indexed | 2024-12-21T19:53:24Z |
| format | Article |
| id | doaj.art-49ef61a14c164120b93e178462010879 |
| institution | Directory Open Access Journal |
| issn | 1415-4757 1678-4685 |
| language | English |
| last_indexed | 2024-12-21T19:53:24Z |
| publishDate | 2008-01-01 |
| publisher | Sociedade Brasileira de Genética |
| record_format | Article |
| series | Genetics and Molecular Biology |
| spelling | doaj.art-49ef61a14c164120b93e1784620108792022-12-21T18:52:09ZengSociedade Brasileira de GenéticaGenetics and Molecular Biology1415-47571678-46852008-01-0131112212710.1590/S1415-47572008000100021Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assaysMarilanda Ferreira BelliniLeonardo Neves CabriotiAna Paula TerezanBerenice Quinzani JordãoLúcia Regina RibeiroMário Sérgio MantovaniFunctional food investigations have demonstrated the presence of substances that could be beneficial to human health when consumed. However, the toxic effects of some substances contained in foods have been determined. Reported medicinal and nutritive properties have led to the extensive commercialization of the basidiomycete fungi Agaricus blazei Murrill (sensu Heinemann), also known as Agaricus brasiliensis Wasser et al., Agaricus subrufescens Peck or the Brazilian medical mushroom (BMM). Different methanolic extract fractions (ME) of this mushroom were submitted to the cytokinesis-block micronucleus (CBMN) clastogenic assay and the hypoxanthine-guanine phosphoribosyl transferase locus (HGPRT) assay for gene mutation, both using Chinese hamster ovary cells clone K1 (CHO-K1). The results suggest that all the fractions tested possess cytotoxic and mutagenic potential but no clastogenic effects. Further information is needed on the biochemical components of the A. blazei methanol fractions to identify any substances with cytotoxic and/or mutagenicity potential. These findings indicate that A. blazei methanolic extract should not be used due to their genotoxicity and care should be taken in the use of A. blazei by the general population until further biochemical characterization of this fungi is completed.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100021Agaricus blazeihgprt locusmicronucleus |
| spellingShingle | Marilanda Ferreira Bellini Leonardo Neves Cabrioti Ana Paula Terezan Berenice Quinzani Jordão Lúcia Regina Ribeiro Mário Sérgio Mantovani Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays Genetics and Molecular Biology Agaricus blazei hgprt locus micronucleus |
| title | Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays |
| title_full | Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays |
| title_fullStr | Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays |
| title_full_unstemmed | Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays |
| title_short | Cytotoxicity and genotoxicity of Agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays |
| title_sort | cytotoxicity and genotoxicity of agaricus blazei methanolic extract fractions assessed using gene and chromosomal mutation assays |
| topic | Agaricus blazei hgprt locus micronucleus |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100021 |
| work_keys_str_mv | AT marilandaferreirabellini cytotoxicityandgenotoxicityofagaricusblazeimethanolicextractfractionsassessedusinggeneandchromosomalmutationassays AT leonardonevescabrioti cytotoxicityandgenotoxicityofagaricusblazeimethanolicextractfractionsassessedusinggeneandchromosomalmutationassays AT anapaulaterezan cytotoxicityandgenotoxicityofagaricusblazeimethanolicextractfractionsassessedusinggeneandchromosomalmutationassays AT berenicequinzanijordao cytotoxicityandgenotoxicityofagaricusblazeimethanolicextractfractionsassessedusinggeneandchromosomalmutationassays AT luciareginaribeiro cytotoxicityandgenotoxicityofagaricusblazeimethanolicextractfractionsassessedusinggeneandchromosomalmutationassays AT mariosergiomantovani cytotoxicityandgenotoxicityofagaricusblazeimethanolicextractfractionsassessedusinggeneandchromosomalmutationassays |