The Role of Toll-like Receptor 2 (TLR2) in the Development and Progression of Hashimoto’s Disease (HD): A Case Study on Female Patients in Poland
Chronic lymphocytic thyroiditis, commonly known as HD, is one of the most common thyroid disorders. Due to the diverse factors affecting the etiopathogenesis of this disease (hormonal disorders and genetic and environmental factors), as well as the direct involvement of the immune system, scientists...
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| author | Maria Klatka Agnieszka Polak Paulina Mertowska Sebastian Mertowski Jakub Łyczba Anna Hymos Izabela Korona-Głowniak Ewelina Grywalska |
| author_facet | Maria Klatka Agnieszka Polak Paulina Mertowska Sebastian Mertowski Jakub Łyczba Anna Hymos Izabela Korona-Głowniak Ewelina Grywalska |
| author_sort | Maria Klatka |
| collection | DOAJ |
| description | Chronic lymphocytic thyroiditis, commonly known as HD, is one of the most common thyroid disorders. Due to the diverse factors affecting the etiopathogenesis of this disease (hormonal disorders and genetic and environmental factors), as well as the direct involvement of the immune system, scientists are increasingly willing to undertake research aimed at explaining the impact of the loss of immune tolerance and reactivity of autoantigens on the development of the disease. One of the directions of research in recent years is the role of the innate immune response, particularly Toll-like receptors (TLRs), in the pathogenesis of HD. The purpose of this study was to determine the importance of Toll-like receptor 2 (TLR2) expression on selected populations of immune cells, namely, monocytes (MONs) and dendritic cells (DCs), in the course of HD. Particular attention was paid to the analysis of TLR2’s correlation with clinical parameters and the possibility its use as a potential biomarker molecule in the diagnostic process. Based on the obtained results, we found a statistically significant increase in the percentage of all analyzed populations of immune cells, i.e., mDC BDCA-1+CD19−, pDC BDCA-1+CD123, classical MONs CD14+CD16−, and non-classical MONs CD14+CD16+ showing on their surface TLR2 expression in patients diagnosed with HD compared to the healthy volunteers. Moreover, in the study group, we noted a more than 6-fold increase in the concentration of the soluble form of TLR2 in plasma compared to healthy patients. In addition, the correlation analysis showed significant positive correlations between the level of TLR2 expression on selected subpopulations of immune cells and biochemical indicators of thyroid function. Based on the obtained results, we can assume that TLR2 may be involved in the immunopathogenesis of HD. |
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| language | English |
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| spelling | doaj.art-49f32a266c87401abace69d1be2e40c22023-11-17T11:32:32ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246534410.3390/ijms24065344The Role of Toll-like Receptor 2 (TLR2) in the Development and Progression of Hashimoto’s Disease (HD): A Case Study on Female Patients in PolandMaria Klatka0Agnieszka Polak1Paulina Mertowska2Sebastian Mertowski3Jakub Łyczba4Anna Hymos5Izabela Korona-Głowniak6Ewelina Grywalska7Department of Pediatric Endocrinology and Diabetology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Endocrinology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Pharmaceutical Microbiology, Medical University of Lublin, 20-093 Lublin, PolandDepartment of Experimental Immunology, Medical University of Lublin, 20-093 Lublin, PolandChronic lymphocytic thyroiditis, commonly known as HD, is one of the most common thyroid disorders. Due to the diverse factors affecting the etiopathogenesis of this disease (hormonal disorders and genetic and environmental factors), as well as the direct involvement of the immune system, scientists are increasingly willing to undertake research aimed at explaining the impact of the loss of immune tolerance and reactivity of autoantigens on the development of the disease. One of the directions of research in recent years is the role of the innate immune response, particularly Toll-like receptors (TLRs), in the pathogenesis of HD. The purpose of this study was to determine the importance of Toll-like receptor 2 (TLR2) expression on selected populations of immune cells, namely, monocytes (MONs) and dendritic cells (DCs), in the course of HD. Particular attention was paid to the analysis of TLR2’s correlation with clinical parameters and the possibility its use as a potential biomarker molecule in the diagnostic process. Based on the obtained results, we found a statistically significant increase in the percentage of all analyzed populations of immune cells, i.e., mDC BDCA-1+CD19−, pDC BDCA-1+CD123, classical MONs CD14+CD16−, and non-classical MONs CD14+CD16+ showing on their surface TLR2 expression in patients diagnosed with HD compared to the healthy volunteers. Moreover, in the study group, we noted a more than 6-fold increase in the concentration of the soluble form of TLR2 in plasma compared to healthy patients. In addition, the correlation analysis showed significant positive correlations between the level of TLR2 expression on selected subpopulations of immune cells and biochemical indicators of thyroid function. Based on the obtained results, we can assume that TLR2 may be involved in the immunopathogenesis of HD.https://www.mdpi.com/1422-0067/24/6/5344Hashimoto’s diseasedendritic cellsmonocytesToll-like receptorsTLR2 |
| spellingShingle | Maria Klatka Agnieszka Polak Paulina Mertowska Sebastian Mertowski Jakub Łyczba Anna Hymos Izabela Korona-Głowniak Ewelina Grywalska The Role of Toll-like Receptor 2 (TLR2) in the Development and Progression of Hashimoto’s Disease (HD): A Case Study on Female Patients in Poland International Journal of Molecular Sciences Hashimoto’s disease dendritic cells monocytes Toll-like receptors TLR2 |
| title | The Role of Toll-like Receptor 2 (TLR2) in the Development and Progression of Hashimoto’s Disease (HD): A Case Study on Female Patients in Poland |
| title_full | The Role of Toll-like Receptor 2 (TLR2) in the Development and Progression of Hashimoto’s Disease (HD): A Case Study on Female Patients in Poland |
| title_fullStr | The Role of Toll-like Receptor 2 (TLR2) in the Development and Progression of Hashimoto’s Disease (HD): A Case Study on Female Patients in Poland |
| title_full_unstemmed | The Role of Toll-like Receptor 2 (TLR2) in the Development and Progression of Hashimoto’s Disease (HD): A Case Study on Female Patients in Poland |
| title_short | The Role of Toll-like Receptor 2 (TLR2) in the Development and Progression of Hashimoto’s Disease (HD): A Case Study on Female Patients in Poland |
| title_sort | role of toll like receptor 2 tlr2 in the development and progression of hashimoto s disease hd a case study on female patients in poland |
| topic | Hashimoto’s disease dendritic cells monocytes Toll-like receptors TLR2 |
| url | https://www.mdpi.com/1422-0067/24/6/5344 |
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