Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs
IntroductionThe establishment of intestinal microbiota and the maintenance of its equilibrium structure plays an important role in Tibetan pigs during different growth stages. Understanding the structure and function of the intestinal microbiota at different growth stages of Tibetan pigs can provide...
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| Format: | Article |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1055146/full |
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| author | Zhenyu Chang Zhenyu Chang Zhenyu Chang Suxue Bo Suxue Bo Qingqing Xiao Qingqing Xiao Yu Wang Yu Wang Xi Wu Xi Wu Yuxuan He Yuxuan He Mujahid Iqbal Yourong Ye Yourong Ye Peng Shang Peng Shang |
| author_facet | Zhenyu Chang Zhenyu Chang Zhenyu Chang Suxue Bo Suxue Bo Qingqing Xiao Qingqing Xiao Yu Wang Yu Wang Xi Wu Xi Wu Yuxuan He Yuxuan He Mujahid Iqbal Yourong Ye Yourong Ye Peng Shang Peng Shang |
| author_sort | Zhenyu Chang |
| collection | DOAJ |
| description | IntroductionThe establishment of intestinal microbiota and the maintenance of its equilibrium structure plays an important role in Tibetan pigs during different growth stages. Understanding the structure and function of the intestinal microbiota at different growth stages of Tibetan pigs can provide a theoretical basis for guiding nutritional regulation and feeding management in different stages.MethodsFecal samples were collected from the Tibetan piglets at different growth stages, and the 16S rRNA was sequenced to analyze the changes of intestinal microbiota.ResultsAlpha and Beta diversity indexes showed that the diversity of the intestinal microbiota did not change during the three growth stages, and the main components of intestinal microbiota were not significantly different. At the phylum level, Firmicutes and Bacteroidetes were dominant and abundant at different growth stages and were not restricted by age. At the genus level, Streptococcus, Lactobacillus, and Bifidobacterium were the most dominant in the TP10d and TP40d groups, Streptococcus was the most dominant in the TP100d group, followed by Treponema_2 and Lactobacillus. Fusobacteria, Gluconobacter, and Synergistetes were found to be specific genera of 10-day-old Tibetan piglets by LEfSe combined with LDA score. The change of diet made Tenericutes and Epsilonbacteraeota, which are closely related to digestive fiber, become specific bacteria at the age of 40 days. With the consumption of oxygen in the intestine, obligate anaerobes, such as Verrucomicrobia, Fibrobacter, and Planctomycetes, were the characteristic genera of 100 days. KEGG function prediction analysis showed that the intestinal microbiota function of Tibetan pigs changed dynamically with the growth and development of Tibetan piglets.DiscussionIn conclusion, the structure and composition of the intestinal microbiota of Tibetan pigs are significantly different at different growth and development stages, which plays an important role in their immune performance. |
| first_indexed | 2024-04-11T07:33:53Z |
| format | Article |
| id | doaj.art-49f442273acc40df97ce7de99f8fb812 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-11T07:33:53Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-49f442273acc40df97ce7de99f8fb8122022-12-22T04:36:48ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-11-011310.3389/fmicb.2022.10551461055146Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigsZhenyu Chang0Zhenyu Chang1Zhenyu Chang2Suxue Bo3Suxue Bo4Qingqing Xiao5Qingqing Xiao6Yu Wang7Yu Wang8Xi Wu9Xi Wu10Yuxuan He11Yuxuan He12Mujahid Iqbal13Yourong Ye14Yourong Ye15Peng Shang16Peng Shang17College of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaThe Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi, ChinaKey Laboratory of Clinical Veterinary Medicine in Tibet, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaCollege of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaThe Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi, ChinaCollege of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaThe Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi, ChinaCollege of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaThe Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi, ChinaCollege of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaThe Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi, ChinaCollege of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaThe Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi, ChinaDepartment of Pathology, Cholistan University of Veterinary and Animal Sciences (CUVAS), Bahawalpur, PakistanCollege of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaThe Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi, ChinaCollege of Animal Science, Tibet Agriculture and Animal Husbandry University, Linzhi, ChinaThe Provincial and Ministerial Co-founded Collaborative Innovation Center for R & D in Tibet Characteristic Agricultural and Animal Husbandry Resources, Linzhi, ChinaIntroductionThe establishment of intestinal microbiota and the maintenance of its equilibrium structure plays an important role in Tibetan pigs during different growth stages. Understanding the structure and function of the intestinal microbiota at different growth stages of Tibetan pigs can provide a theoretical basis for guiding nutritional regulation and feeding management in different stages.MethodsFecal samples were collected from the Tibetan piglets at different growth stages, and the 16S rRNA was sequenced to analyze the changes of intestinal microbiota.ResultsAlpha and Beta diversity indexes showed that the diversity of the intestinal microbiota did not change during the three growth stages, and the main components of intestinal microbiota were not significantly different. At the phylum level, Firmicutes and Bacteroidetes were dominant and abundant at different growth stages and were not restricted by age. At the genus level, Streptococcus, Lactobacillus, and Bifidobacterium were the most dominant in the TP10d and TP40d groups, Streptococcus was the most dominant in the TP100d group, followed by Treponema_2 and Lactobacillus. Fusobacteria, Gluconobacter, and Synergistetes were found to be specific genera of 10-day-old Tibetan piglets by LEfSe combined with LDA score. The change of diet made Tenericutes and Epsilonbacteraeota, which are closely related to digestive fiber, become specific bacteria at the age of 40 days. With the consumption of oxygen in the intestine, obligate anaerobes, such as Verrucomicrobia, Fibrobacter, and Planctomycetes, were the characteristic genera of 100 days. KEGG function prediction analysis showed that the intestinal microbiota function of Tibetan pigs changed dynamically with the growth and development of Tibetan piglets.DiscussionIn conclusion, the structure and composition of the intestinal microbiota of Tibetan pigs are significantly different at different growth and development stages, which plays an important role in their immune performance.https://www.frontiersin.org/articles/10.3389/fmicb.2022.1055146/fullTibetan piggrowth stageintestinal microbiota16S rRNA sequencingTibet |
| spellingShingle | Zhenyu Chang Zhenyu Chang Zhenyu Chang Suxue Bo Suxue Bo Qingqing Xiao Qingqing Xiao Yu Wang Yu Wang Xi Wu Xi Wu Yuxuan He Yuxuan He Mujahid Iqbal Yourong Ye Yourong Ye Peng Shang Peng Shang Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs Frontiers in Microbiology Tibetan pig growth stage intestinal microbiota 16S rRNA sequencing Tibet |
| title | Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs |
| title_full | Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs |
| title_fullStr | Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs |
| title_full_unstemmed | Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs |
| title_short | Remodeling of the microbiota improves the environmental adaptability and disease resistance in Tibetan pigs |
| title_sort | remodeling of the microbiota improves the environmental adaptability and disease resistance in tibetan pigs |
| topic | Tibetan pig growth stage intestinal microbiota 16S rRNA sequencing Tibet |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.1055146/full |
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