Identification of miRNAs of Strongyloides stercoralis L1 and iL3 larvae isolated from human stool
Abstract Strongyloidiasis is a neglected tropical disease caused by the soil-transmitted nematode by Strongyloides stercoralis, that affects approximately 600 million people worldwide. In immunosuppressed individuals disseminated strongyloidiasis can rapidly lead to fatal outcomes. There is no gold...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-06-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-14185-y |
| _version_ | 1828821815924883456 |
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| author | Elena Pomari Giovanni Malerba Laura Veschetti Alessandra Franceschi Lucas Moron Dalla Tor Michela Deiana Monica Degani Manuela Mistretta Cristina Patuzzo Andrea Ragusa Antonio Mori Zeno Bisoffi Dora Buonfrate |
| author_facet | Elena Pomari Giovanni Malerba Laura Veschetti Alessandra Franceschi Lucas Moron Dalla Tor Michela Deiana Monica Degani Manuela Mistretta Cristina Patuzzo Andrea Ragusa Antonio Mori Zeno Bisoffi Dora Buonfrate |
| author_sort | Elena Pomari |
| collection | DOAJ |
| description | Abstract Strongyloidiasis is a neglected tropical disease caused by the soil-transmitted nematode by Strongyloides stercoralis, that affects approximately 600 million people worldwide. In immunosuppressed individuals disseminated strongyloidiasis can rapidly lead to fatal outcomes. There is no gold standard for diagnosing strongyloidiasis, and infections are frequently misdiagnosed. A better understanding of the molecular biology of this parasite can be useful for example for the discovery of potential new biomarkers. Interestingly, recent evidence showed the presence of small RNAs in Strongyloididae, but no data was provided for S. stercoralis. In this study, we present the first identification of miRNAs of both L1 and iL3 larval stages of S. stercoralis. For our purpose, the aims were: (i) to analyse the miRNome of L1 and iL3 S. stercoralis and to identify potential miRNAs of this nematode, (ii) to obtain the mRNAs profiles in these two larval stages and (iii) to predict potential miRNA target sites in mRNA sequences. Total RNA was isolated from L1 and iL3 collected from the stool of 5 infected individuals. For the miRNAs analysis, we used miRDeep2 software and a pipeline of bio-informatic tools to construct a catalog of a total of 385 sequences. Among these, 53% were common to S. ratti, 19% to S. papillosus, 1% to Caenorhabditis elegans and 44% were novel. Using a differential analysis between the larval stages, we observed 6 suggestive modulated miRNAs (STR-MIR-34A-3P, STR-MIR-8397-3P, STR-MIR-34B-3P and STR-MIR-34C-3P expressed more in iL3, and STR-MIR-7880H-5P and STR-MIR-7880M-5P expressed more in L1). Along with this analysis, we obtained also the mRNAs profiles in the same samples of larvae. Multiple testing found 81 statistically significant mRNAs of the total 1553 obtained (FDR < 0.05; 32 genes expressed more in L1 than iL3; 49 genes expressed more in L3 than iL1). Finally, we found 33 predicted mRNA targets of the modulated miRNAs, providing relevant data for a further validation to better understand the role of these small molecules in the larval stages and their valuein clinical diagnostics. |
| first_indexed | 2024-12-12T13:01:46Z |
| format | Article |
| id | doaj.art-49f5f249087146e7bb3ce50d68ed7bc1 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-12T13:01:46Z |
| publishDate | 2022-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-49f5f249087146e7bb3ce50d68ed7bc12022-12-22T00:23:46ZengNature PortfolioScientific Reports2045-23222022-06-0112111210.1038/s41598-022-14185-yIdentification of miRNAs of Strongyloides stercoralis L1 and iL3 larvae isolated from human stoolElena Pomari0Giovanni Malerba1Laura Veschetti2Alessandra Franceschi3Lucas Moron Dalla Tor4Michela Deiana5Monica Degani6Manuela Mistretta7Cristina Patuzzo8Andrea Ragusa9Antonio Mori10Zeno Bisoffi11Dora Buonfrate12Department of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria HospitalDepartment of Neurosciences, Biomedicine and Movement Sciences, University of VeronaDepartment of Neurosciences, Biomedicine and Movement Sciences, University of VeronaDepartment of Neurosciences, Biomedicine and Movement Sciences, University of VeronaDepartment of Medicine and Surgery, University of ParmaDepartment of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria HospitalDepartment of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria HospitalDepartment of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria HospitalDepartment of Neurosciences, Biomedicine and Movement Sciences, University of VeronaDepartment of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria HospitalDepartment of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria HospitalDepartment of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria HospitalDepartment of Infectious-Tropical Diseases and Microbiology, IRCCS Sacro Cuore Don Calabria HospitalAbstract Strongyloidiasis is a neglected tropical disease caused by the soil-transmitted nematode by Strongyloides stercoralis, that affects approximately 600 million people worldwide. In immunosuppressed individuals disseminated strongyloidiasis can rapidly lead to fatal outcomes. There is no gold standard for diagnosing strongyloidiasis, and infections are frequently misdiagnosed. A better understanding of the molecular biology of this parasite can be useful for example for the discovery of potential new biomarkers. Interestingly, recent evidence showed the presence of small RNAs in Strongyloididae, but no data was provided for S. stercoralis. In this study, we present the first identification of miRNAs of both L1 and iL3 larval stages of S. stercoralis. For our purpose, the aims were: (i) to analyse the miRNome of L1 and iL3 S. stercoralis and to identify potential miRNAs of this nematode, (ii) to obtain the mRNAs profiles in these two larval stages and (iii) to predict potential miRNA target sites in mRNA sequences. Total RNA was isolated from L1 and iL3 collected from the stool of 5 infected individuals. For the miRNAs analysis, we used miRDeep2 software and a pipeline of bio-informatic tools to construct a catalog of a total of 385 sequences. Among these, 53% were common to S. ratti, 19% to S. papillosus, 1% to Caenorhabditis elegans and 44% were novel. Using a differential analysis between the larval stages, we observed 6 suggestive modulated miRNAs (STR-MIR-34A-3P, STR-MIR-8397-3P, STR-MIR-34B-3P and STR-MIR-34C-3P expressed more in iL3, and STR-MIR-7880H-5P and STR-MIR-7880M-5P expressed more in L1). Along with this analysis, we obtained also the mRNAs profiles in the same samples of larvae. Multiple testing found 81 statistically significant mRNAs of the total 1553 obtained (FDR < 0.05; 32 genes expressed more in L1 than iL3; 49 genes expressed more in L3 than iL1). Finally, we found 33 predicted mRNA targets of the modulated miRNAs, providing relevant data for a further validation to better understand the role of these small molecules in the larval stages and their valuein clinical diagnostics.https://doi.org/10.1038/s41598-022-14185-y |
| spellingShingle | Elena Pomari Giovanni Malerba Laura Veschetti Alessandra Franceschi Lucas Moron Dalla Tor Michela Deiana Monica Degani Manuela Mistretta Cristina Patuzzo Andrea Ragusa Antonio Mori Zeno Bisoffi Dora Buonfrate Identification of miRNAs of Strongyloides stercoralis L1 and iL3 larvae isolated from human stool Scientific Reports |
| title | Identification of miRNAs of Strongyloides stercoralis L1 and iL3 larvae isolated from human stool |
| title_full | Identification of miRNAs of Strongyloides stercoralis L1 and iL3 larvae isolated from human stool |
| title_fullStr | Identification of miRNAs of Strongyloides stercoralis L1 and iL3 larvae isolated from human stool |
| title_full_unstemmed | Identification of miRNAs of Strongyloides stercoralis L1 and iL3 larvae isolated from human stool |
| title_short | Identification of miRNAs of Strongyloides stercoralis L1 and iL3 larvae isolated from human stool |
| title_sort | identification of mirnas of strongyloides stercoralis l1 and il3 larvae isolated from human stool |
| url | https://doi.org/10.1038/s41598-022-14185-y |
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