A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression
Prostate cancer is the most common cancer in the male population, carrying a significant disease burden. PSA is a widely available screening tools for this disease. Current screen-printed carbon electrode (SPCE)-based biosensors use a two-pronged probe approach to capture urinary miRNA. We were able...
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MDPI AG
2022-12-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/9/12/803 |
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| author | Yueh-Er Chiou Kai-Jie Yu Sow-Neng Pang Yan-Lin Yang See-Tong Pang Wen-Hui Weng |
| author_facet | Yueh-Er Chiou Kai-Jie Yu Sow-Neng Pang Yan-Lin Yang See-Tong Pang Wen-Hui Weng |
| author_sort | Yueh-Er Chiou |
| collection | DOAJ |
| description | Prostate cancer is the most common cancer in the male population, carrying a significant disease burden. PSA is a widely available screening tools for this disease. Current screen-printed carbon electrode (SPCE)-based biosensors use a two-pronged probe approach to capture urinary miRNA. We were able to successfully detect specific exosomal miRNAs (exomiRs) in the urine of patients with prostate cancer, including exomiR-451 and exomiR-21, and used electrochemistry for measurement and analysis. Our results significantly reaffirmed the presence of exomiR-451 in urine and that a CV value higher than 220 nA is capable of identifying the presence of disease (<i>p</i>-value = 0.005). Similar results were further proven by a PAS greater than 4 (<i>p</i>-value = 0.001). Moreover, a higher urinary exomiR-21 was observed in the high-T3b stage; this significantly decreased following tumor removal (<i>p</i>-values were 0.016 and 0.907, respectively). According to analysis of the correlation with tumor metastasis, a higher exomiR-21 was associated with lymphatic metastasis (p-value 0.042), and higher exomiR-461 expression was correlated with tumor stage (<i>p</i>-value 0.031), demonstrating that the present exomiR biosensor can usefully predict tumor progression. In conclusion, this biosensor represents an easy-to-use, non-invasive screening tool that is both sensitive and specific. We strongly believe that this can be used in conjunction with PSA for the screening of prostate cancer. |
| first_indexed | 2024-03-09T17:19:06Z |
| format | Article |
| id | doaj.art-49f613c5280c4ee6bb55167dacbc70ef |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-09T17:19:06Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-49f613c5280c4ee6bb55167dacbc70ef2023-11-24T13:21:18ZengMDPI AGBioengineering2306-53542022-12-0191280310.3390/bioengineering9120803A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer ProgressionYueh-Er Chiou0Kai-Jie Yu1Sow-Neng Pang2Yan-Lin Yang3See-Tong Pang4Wen-Hui Weng5Department of Nursing, College of Medicine, Fu Jen Catholic University, New Taipei City 242, TaiwanDepartment of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, TaiwanDepartment of Emergency Medicine, Monash Medical Centre, Melbourne, VIC 3083, AustraliaDepartment of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, TaiwanDivision of Urology, Department of Surgery, Chang Gung Memorial Hospital, Taoyuan 33305, TaiwanDepartment of Chemical Engineering and Biotechnology and Graduate Institute of Biochemical and Biomedical Engineering, National Taipei University of Technology, Taipei City 106, TaiwanProstate cancer is the most common cancer in the male population, carrying a significant disease burden. PSA is a widely available screening tools for this disease. Current screen-printed carbon electrode (SPCE)-based biosensors use a two-pronged probe approach to capture urinary miRNA. We were able to successfully detect specific exosomal miRNAs (exomiRs) in the urine of patients with prostate cancer, including exomiR-451 and exomiR-21, and used electrochemistry for measurement and analysis. Our results significantly reaffirmed the presence of exomiR-451 in urine and that a CV value higher than 220 nA is capable of identifying the presence of disease (<i>p</i>-value = 0.005). Similar results were further proven by a PAS greater than 4 (<i>p</i>-value = 0.001). Moreover, a higher urinary exomiR-21 was observed in the high-T3b stage; this significantly decreased following tumor removal (<i>p</i>-values were 0.016 and 0.907, respectively). According to analysis of the correlation with tumor metastasis, a higher exomiR-21 was associated with lymphatic metastasis (p-value 0.042), and higher exomiR-461 expression was correlated with tumor stage (<i>p</i>-value 0.031), demonstrating that the present exomiR biosensor can usefully predict tumor progression. In conclusion, this biosensor represents an easy-to-use, non-invasive screening tool that is both sensitive and specific. We strongly believe that this can be used in conjunction with PSA for the screening of prostate cancer.https://www.mdpi.com/2306-5354/9/12/803prostate cancerSPCEmicroRNAbiosensor |
| spellingShingle | Yueh-Er Chiou Kai-Jie Yu Sow-Neng Pang Yan-Lin Yang See-Tong Pang Wen-Hui Weng A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression Bioengineering prostate cancer SPCE microRNA biosensor |
| title | A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression |
| title_full | A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression |
| title_fullStr | A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression |
| title_full_unstemmed | A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression |
| title_short | A Highly Sensitive Urinary Exosomal miRNAs Biosensor Applied to Evaluation of Prostate Cancer Progression |
| title_sort | highly sensitive urinary exosomal mirnas biosensor applied to evaluation of prostate cancer progression |
| topic | prostate cancer SPCE microRNA biosensor |
| url | https://www.mdpi.com/2306-5354/9/12/803 |
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