Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation
Posidonia oceanica (L.) Delile is a marine plant with interesting biological properties potentially ascribed to the synergistic combination of bioactive compounds. Our previously described extract, obtained from the leaves of P. oceanica, showed the ability to impair HT1080 cell migration by targeti...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-04-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/16/4/137 |
| _version_ | 1811184765127098368 |
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| author | Manuela Leri Matteo Ramazzotti Marzia Vasarri Sara Peri Emanuela Barletta Carlo Pretti Donatella Degl’Innocenti |
| author_facet | Manuela Leri Matteo Ramazzotti Marzia Vasarri Sara Peri Emanuela Barletta Carlo Pretti Donatella Degl’Innocenti |
| author_sort | Manuela Leri |
| collection | DOAJ |
| description | Posidonia oceanica (L.) Delile is a marine plant with interesting biological properties potentially ascribed to the synergistic combination of bioactive compounds. Our previously described extract, obtained from the leaves of P. oceanica, showed the ability to impair HT1080 cell migration by targeting both expression and activity of gelatinases. Commonly, the lack of knowledge about the mechanism of action of phytocomplexes may be an obstacle regarding their therapeutic use and development. The aim of this study was to gain insight into the molecular signaling through which such bioactive compounds impact on malignant cell migration and gelatinolytic activity. The increase in autophagic vacuoles detected by confocal microscopy suggested an enhancement of autophagy in a time and dose dependent manner. This autophagy activation was further confirmed by monitoring pivotal markers of autophagy signaling as well as by evidencing an increase in IGF-1R accumulation on cell membranes. Taken together, our results confirm that the P. oceanica phytocomplex is a promising reservoir of potent and cell safe molecules able to defend against malignancies and other diseases in which gelatinases play a major role in progression. In conclusion, the attractive properties of this phytocomplex may be of industrial interest in regard to the development of novel health-promoting and pharmacological products for the treatment or prevention of several diseases. |
| first_indexed | 2024-04-11T13:19:03Z |
| format | Article |
| id | doaj.art-49f6a3bcf2884c10891272a90ed7751f |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:19:03Z |
| publishDate | 2018-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-49f6a3bcf2884c10891272a90ed7751f2022-12-22T04:22:18ZengMDPI AGMarine Drugs1660-33972018-04-0116413710.3390/md16040137md16040137Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy ModulationManuela Leri0Matteo Ramazzotti1Marzia Vasarri2Sara Peri3Emanuela Barletta4Carlo Pretti5Donatella Degl’Innocenti6Dipartimento di Scienze Biomediche, Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, viale Morgagni 50, 50134 Firenze, ItalyDipartimento di Scienze Biomediche, Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, viale Morgagni 50, 50134 Firenze, ItalyDipartimento di Scienze Biomediche, Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, viale Morgagni 50, 50134 Firenze, ItalyDipartimento di Scienze Biomediche, Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, viale Morgagni 50, 50134 Firenze, ItalyDipartimento di Scienze Biomediche, Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, viale Morgagni 50, 50134 Firenze, ItalyDipartimento di Scienze Veterinarie, Università degli Studi di Pisa, viale delle Piagge 2, 56124 Pisa, ItalyDipartimento di Scienze Biomediche, Sperimentali e Cliniche “Mario Serio”, Università degli Studi di Firenze, viale Morgagni 50, 50134 Firenze, ItalyPosidonia oceanica (L.) Delile is a marine plant with interesting biological properties potentially ascribed to the synergistic combination of bioactive compounds. Our previously described extract, obtained from the leaves of P. oceanica, showed the ability to impair HT1080 cell migration by targeting both expression and activity of gelatinases. Commonly, the lack of knowledge about the mechanism of action of phytocomplexes may be an obstacle regarding their therapeutic use and development. The aim of this study was to gain insight into the molecular signaling through which such bioactive compounds impact on malignant cell migration and gelatinolytic activity. The increase in autophagic vacuoles detected by confocal microscopy suggested an enhancement of autophagy in a time and dose dependent manner. This autophagy activation was further confirmed by monitoring pivotal markers of autophagy signaling as well as by evidencing an increase in IGF-1R accumulation on cell membranes. Taken together, our results confirm that the P. oceanica phytocomplex is a promising reservoir of potent and cell safe molecules able to defend against malignancies and other diseases in which gelatinases play a major role in progression. In conclusion, the attractive properties of this phytocomplex may be of industrial interest in regard to the development of novel health-promoting and pharmacological products for the treatment or prevention of several diseases.http://www.mdpi.com/1660-3397/16/4/137Posidonia oceanicaautophagycell migrationgelatinaseHT1080 cell line |
| spellingShingle | Manuela Leri Matteo Ramazzotti Marzia Vasarri Sara Peri Emanuela Barletta Carlo Pretti Donatella Degl’Innocenti Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation Marine Drugs Posidonia oceanica autophagy cell migration gelatinase HT1080 cell line |
| title | Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation |
| title_full | Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation |
| title_fullStr | Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation |
| title_full_unstemmed | Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation |
| title_short | Bioactive Compounds from Posidonia oceanica (L.) Delile Impair Malignant Cell Migration through Autophagy Modulation |
| title_sort | bioactive compounds from posidonia oceanica l delile impair malignant cell migration through autophagy modulation |
| topic | Posidonia oceanica autophagy cell migration gelatinase HT1080 cell line |
| url | http://www.mdpi.com/1660-3397/16/4/137 |
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