Transcriptome Analysis of Salt Stress in <i>Hibiscus hamabo</i> Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing
<i>Hibiscus hamabo</i> Sieb. et Zucc is an important semi-mangrove plant with great morphological features and strong salt resistance. In this study, by combining single molecule real time and next-generation sequencing technologies, we explored the transcriptomic changes in the roots of...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/1/138 |
| _version_ | 1797498869820424192 |
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| author | Longjie Ni Zhiquan Wang Xiangdong Liu Shuting Wu Jianfeng Hua Yunlong Yin Huogen Li Chunsun Gu |
| author_facet | Longjie Ni Zhiquan Wang Xiangdong Liu Shuting Wu Jianfeng Hua Yunlong Yin Huogen Li Chunsun Gu |
| author_sort | Longjie Ni |
| collection | DOAJ |
| description | <i>Hibiscus hamabo</i> Sieb. et Zucc is an important semi-mangrove plant with great morphological features and strong salt resistance. In this study, by combining single molecule real time and next-generation sequencing technologies, we explored the transcriptomic changes in the roots of salt stressed <i>H</i><i>. hamabo</i>. A total of 94,562 unigenes were obtained by clustering the same isoforms using the PacBio RSII platform, and 2269 differentially expressed genes were obtained under salt stress using the Illumina platform. There were 519 differentially expressed genes co-expressed at each treatment time point under salt stress, and these genes were found to be enriched in ion signal transduction and plant hormone signal transduction. We used <i>Arabidopsis thaliana</i> (L.) Heynh. transformation to confirm the function of the <i>HhWRKY79</i> gene and discovered that overexpression enhanced salt tolerance. The full-length transcripts generated in this study provide a full characterization of the transcriptome of <i>H</i><i>. hamabo</i> and may be useful in mining new salt stress-related genes specific to this species, while facilitating the understanding of the salt tolerance mechanisms. |
| first_indexed | 2024-03-10T03:39:28Z |
| format | Article |
| id | doaj.art-49f91e22ecbe4ff38c6c87017a898194 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:39:28Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-49f91e22ecbe4ff38c6c87017a8981942023-11-23T11:35:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0123113810.3390/ijms23010138Transcriptome Analysis of Salt Stress in <i>Hibiscus hamabo</i> Sieb. et Zucc Based on Pacbio Full-Length Transcriptome SequencingLongjie Ni0Zhiquan Wang1Xiangdong Liu2Shuting Wu3Jianfeng Hua4Yunlong Yin5Huogen Li6Chunsun Gu7Institute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, ChinaCollege of Forest Sciences, Nanjing Forestry University, Nanjing 210037, ChinaInstitute of Botany, Jiangsu Province and Chinese Academy of Sciences, Nanjing 210014, China<i>Hibiscus hamabo</i> Sieb. et Zucc is an important semi-mangrove plant with great morphological features and strong salt resistance. In this study, by combining single molecule real time and next-generation sequencing technologies, we explored the transcriptomic changes in the roots of salt stressed <i>H</i><i>. hamabo</i>. A total of 94,562 unigenes were obtained by clustering the same isoforms using the PacBio RSII platform, and 2269 differentially expressed genes were obtained under salt stress using the Illumina platform. There were 519 differentially expressed genes co-expressed at each treatment time point under salt stress, and these genes were found to be enriched in ion signal transduction and plant hormone signal transduction. We used <i>Arabidopsis thaliana</i> (L.) Heynh. transformation to confirm the function of the <i>HhWRKY79</i> gene and discovered that overexpression enhanced salt tolerance. The full-length transcripts generated in this study provide a full characterization of the transcriptome of <i>H</i><i>. hamabo</i> and may be useful in mining new salt stress-related genes specific to this species, while facilitating the understanding of the salt tolerance mechanisms.https://www.mdpi.com/1422-0067/23/1/138full-length transcriptomesalt stressWRKY transcription factorsemi-mangrove plant<i>Hibiscus hamabo</i> Sieb. et Zucc |
| spellingShingle | Longjie Ni Zhiquan Wang Xiangdong Liu Shuting Wu Jianfeng Hua Yunlong Yin Huogen Li Chunsun Gu Transcriptome Analysis of Salt Stress in <i>Hibiscus hamabo</i> Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing International Journal of Molecular Sciences full-length transcriptome salt stress WRKY transcription factor semi-mangrove plant <i>Hibiscus hamabo</i> Sieb. et Zucc |
| title | Transcriptome Analysis of Salt Stress in <i>Hibiscus hamabo</i> Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing |
| title_full | Transcriptome Analysis of Salt Stress in <i>Hibiscus hamabo</i> Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing |
| title_fullStr | Transcriptome Analysis of Salt Stress in <i>Hibiscus hamabo</i> Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing |
| title_full_unstemmed | Transcriptome Analysis of Salt Stress in <i>Hibiscus hamabo</i> Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing |
| title_short | Transcriptome Analysis of Salt Stress in <i>Hibiscus hamabo</i> Sieb. et Zucc Based on Pacbio Full-Length Transcriptome Sequencing |
| title_sort | transcriptome analysis of salt stress in i hibiscus hamabo i sieb et zucc based on pacbio full length transcriptome sequencing |
| topic | full-length transcriptome salt stress WRKY transcription factor semi-mangrove plant <i>Hibiscus hamabo</i> Sieb. et Zucc |
| url | https://www.mdpi.com/1422-0067/23/1/138 |
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