Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines
Two genes, <i>CsLRR-RPK2</i> (<i>CsGy5G015660</i>) and <i>CsaMLO8</i> (<i>Csa5G623470</i>), have been considered as powdery mildew (PM) resistance genes in cucumbers. In this study, we evaluated the involvement of the alleles of these two genes in PM r...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-10-01
|
| Series: | Agronomy |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4395/11/11/2191 |
| _version_ | 1797511560937078784 |
|---|---|
| author | Mahdi Badri Anarjan Ikhyun Bae Sanghyeob Lee |
| author_facet | Mahdi Badri Anarjan Ikhyun Bae Sanghyeob Lee |
| author_sort | Mahdi Badri Anarjan |
| collection | DOAJ |
| description | Two genes, <i>CsLRR-RPK2</i> (<i>CsGy5G015660</i>) and <i>CsaMLO8</i> (<i>Csa5G623470</i>), have been considered as powdery mildew (PM) resistance genes in cucumbers. In this study, we evaluated the involvement of the alleles of these two genes in PM resistance in 100 commercial Korean cucumber inbred lines. To achieve this, we developed cleaved amplified polymorphic sequences (CAPS) and InDel markers from <i>CsLRR-RPK2</i> and <i>CsaMLO8</i>. Genotyping analysis indicated that the <i>CsLRR-RPK2</i>-CAPS marker showed a stronger correlation with the PM-resistant phenotype, with an 84% consistency compared to the <i>CsaMLO8</i>-InDel marker. The use of the <i>CsaMLO8</i>-InDel marker showed a 70% consistency between phenotype and genotype results. It was proposed that the <i>CsLRR-RPK2</i>-CAPS marker successfully eliminated PM-susceptible inbred lines, since both genotype and phenotype results were 100% identical. Furthermore, the present study revealed that the introduction of one of these alleles is probably enough to confer PM resistance in cucumbers. However, seven PM-resistant inbred lines harbored either <i>CsaMLO8</i> or <i>CsLRR-RPK2</i> alleles, indicating that there is another PM-resistant resource(s) besides <i>CsaMLO8-</i> and <i>CsLRR-RPK2</i>–originated resistance in the commercial Korean inbred lines. Our results provide reliable evidence confirming two PM-resistant candidate genes for the detection of PM resistance resources in cucumber inbred lines. |
| first_indexed | 2024-03-10T05:46:59Z |
| format | Article |
| id | doaj.art-49fa6059cf314de195aa71891c0c672e |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-10T05:46:59Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-49fa6059cf314de195aa71891c0c672e2023-11-22T22:01:52ZengMDPI AGAgronomy2073-43952021-10-011111219110.3390/agronomy11112191Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred LinesMahdi Badri Anarjan0Ikhyun Bae1Sanghyeob Lee2Plant Genomics Laboratory, Department of Bio-Resource Engineering, College of Life Sciences, Sejong University, Seoul 05006, KoreaBreeding Research Institute, DONGOH SEED, Icheon 17405, KoreaPlant Genomics Laboratory, Department of Bio-Resource Engineering, College of Life Sciences, Sejong University, Seoul 05006, KoreaTwo genes, <i>CsLRR-RPK2</i> (<i>CsGy5G015660</i>) and <i>CsaMLO8</i> (<i>Csa5G623470</i>), have been considered as powdery mildew (PM) resistance genes in cucumbers. In this study, we evaluated the involvement of the alleles of these two genes in PM resistance in 100 commercial Korean cucumber inbred lines. To achieve this, we developed cleaved amplified polymorphic sequences (CAPS) and InDel markers from <i>CsLRR-RPK2</i> and <i>CsaMLO8</i>. Genotyping analysis indicated that the <i>CsLRR-RPK2</i>-CAPS marker showed a stronger correlation with the PM-resistant phenotype, with an 84% consistency compared to the <i>CsaMLO8</i>-InDel marker. The use of the <i>CsaMLO8</i>-InDel marker showed a 70% consistency between phenotype and genotype results. It was proposed that the <i>CsLRR-RPK2</i>-CAPS marker successfully eliminated PM-susceptible inbred lines, since both genotype and phenotype results were 100% identical. Furthermore, the present study revealed that the introduction of one of these alleles is probably enough to confer PM resistance in cucumbers. However, seven PM-resistant inbred lines harbored either <i>CsaMLO8</i> or <i>CsLRR-RPK2</i> alleles, indicating that there is another PM-resistant resource(s) besides <i>CsaMLO8-</i> and <i>CsLRR-RPK2</i>–originated resistance in the commercial Korean inbred lines. Our results provide reliable evidence confirming two PM-resistant candidate genes for the detection of PM resistance resources in cucumber inbred lines.https://www.mdpi.com/2073-4395/11/11/2191<i>Cucumis sativus</i> L.<i>Podosphaera xanthii</i>PM-resistance resource<i>MLO</i><i>LRR-RPK2</i> |
| spellingShingle | Mahdi Badri Anarjan Ikhyun Bae Sanghyeob Lee Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines Agronomy <i>Cucumis sativus</i> L. <i>Podosphaera xanthii</i> PM-resistance resource <i>MLO</i> <i>LRR-RPK2</i> |
| title | Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines |
| title_full | Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines |
| title_fullStr | Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines |
| title_full_unstemmed | Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines |
| title_short | Marker-Assisted Evaluation of Two Powdery Mildew Resistance Candidate Genes in Korean Cucumber Inbred Lines |
| title_sort | marker assisted evaluation of two powdery mildew resistance candidate genes in korean cucumber inbred lines |
| topic | <i>Cucumis sativus</i> L. <i>Podosphaera xanthii</i> PM-resistance resource <i>MLO</i> <i>LRR-RPK2</i> |
| url | https://www.mdpi.com/2073-4395/11/11/2191 |
| work_keys_str_mv | AT mahdibadrianarjan markerassistedevaluationoftwopowderymildewresistancecandidategenesinkoreancucumberinbredlines AT ikhyunbae markerassistedevaluationoftwopowderymildewresistancecandidategenesinkoreancucumberinbredlines AT sanghyeoblee markerassistedevaluationoftwopowderymildewresistancecandidategenesinkoreancucumberinbredlines |