Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human Astroglia
Background: Sialic acid (SA) is presented in all cells membrane of vertebrates, and its level in the human brain is much higher than other body tissues. Studies have shown that, in addition to oxidative stress, increasing the amount of SA can also lead to the development of neurological diseases inc...
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| Format: | Article |
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Isfahan University of Medical Sciences
2019-06-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/11536 |
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| author | Rahim Moradi Zahra Nazeri shirin Azizidoost Maraym Cheraghzadeh Alireza Kheirollah |
| author_facet | Rahim Moradi Zahra Nazeri shirin Azizidoost Maraym Cheraghzadeh Alireza Kheirollah |
| author_sort | Rahim Moradi |
| collection | DOAJ |
| description | Background: Sialic acid (SA) is presented in all cells membrane of vertebrates, and its level in the human brain is much higher than other body tissues. Studies have shown that, in addition to oxidative stress, increasing the amount of SA can also lead to the development of neurological diseases including Alzheimer's disease. Therefore, the present study was designed to evaluate the effect of SA on malondialdehyde (MDA) production levels, as a lipid peroxidation product, in human astroglia.
Methods: The human astroglias were cultured in Dulbecco's Modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), and cells were treated with different doses of SA. MDA was measured using thiobarbituric acid (TBA) protocol, and the results were analyzed using SPSS software.
Findings: The production of MDA in treated cells with 200, 500, and 1000 μg/ml of SA significantly increased compared to the control group. It also significantly increased when the cells were treated with 200 μg/ml of SA at 12, 16, and 24 hours incubation.
Conclusion: Many studies have been conducted on neurological disorders; however their mechanism of occurrence has not yet been fully elucidated. With regard to the role of SA in inflammation, our results suggest that SA can cause pathological conditions and oxidative stress followed by MDA elevation; which is effective in the development of neurological disorders like Alzheimer's. The role of SA and its effects need further studies. |
| first_indexed | 2024-03-12T06:24:47Z |
| format | Article |
| id | doaj.art-49fd3cb04f5c4832a9212987f06397af |
| institution | Directory Open Access Journal |
| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T06:24:47Z |
| publishDate | 2019-06-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-49fd3cb04f5c4832a9212987f06397af2023-09-03T01:56:54ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2019-06-013752026326910.22122/jims.v37i520.115363385Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human AstrogliaRahim Moradi0Zahra Nazeri1shirin Azizidoost2Maraym Cheraghzadeh3Alireza Kheirollah4MSc Student, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranMSc Student, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranPhD Student, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranAssistant Professor, Department of Biochemistry, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranAssociate Professor, Department of Biochemistry, School of Medicine AND Cellular and Molecular Research Center, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground: Sialic acid (SA) is presented in all cells membrane of vertebrates, and its level in the human brain is much higher than other body tissues. Studies have shown that, in addition to oxidative stress, increasing the amount of SA can also lead to the development of neurological diseases including Alzheimer's disease. Therefore, the present study was designed to evaluate the effect of SA on malondialdehyde (MDA) production levels, as a lipid peroxidation product, in human astroglia. Methods: The human astroglias were cultured in Dulbecco's Modified Eagle's medium (DMEM) with 10% fetal bovine serum (FBS), and cells were treated with different doses of SA. MDA was measured using thiobarbituric acid (TBA) protocol, and the results were analyzed using SPSS software. Findings: The production of MDA in treated cells with 200, 500, and 1000 μg/ml of SA significantly increased compared to the control group. It also significantly increased when the cells were treated with 200 μg/ml of SA at 12, 16, and 24 hours incubation. Conclusion: Many studies have been conducted on neurological disorders; however their mechanism of occurrence has not yet been fully elucidated. With regard to the role of SA in inflammation, our results suggest that SA can cause pathological conditions and oxidative stress followed by MDA elevation; which is effective in the development of neurological disorders like Alzheimer's. The role of SA and its effects need further studies.http://jims.mui.ac.ir/index.php/jims/article/view/11536Sialic acidOxidative stressMalondialdehydeHumanAstroglia |
| spellingShingle | Rahim Moradi Zahra Nazeri shirin Azizidoost Maraym Cheraghzadeh Alireza Kheirollah Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human Astroglia مجله دانشکده پزشکی اصفهان Sialic acid Oxidative stress Malondialdehyde Human Astroglia |
| title | Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human Astroglia |
| title_full | Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human Astroglia |
| title_fullStr | Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human Astroglia |
| title_full_unstemmed | Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human Astroglia |
| title_short | Evaluating the Level of Malondialdehyde (MDA) in Sialic Acid-Treated Human Astroglia |
| title_sort | evaluating the level of malondialdehyde mda in sialic acid treated human astroglia |
| topic | Sialic acid Oxidative stress Malondialdehyde Human Astroglia |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/11536 |
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