Characterization of the camel pox virus strain used in producing camel pox virus vaccine
Background: The camel pox virus is a widespread infectious viral disease of camels. It is necessary to conduct research on new strains for the development of vaccines. Aim: The research aims to characterize a novel strain isolated from the camel pox virus used to produce a camel pox virus vaccine....
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Tripoli University
2023-05-01
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| Series: | Open Veterinary Journal |
| Subjects: | |
| Online Access: | https://www.openveterinaryjournal.com/?mno=93653 |
| _version_ | 1797794217588686848 |
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| author | Kydyrbay Maikhin Maxat Berdikulov Abdikalyk Abishov Yerlan Pazylov Gulzhan Mussayeva Slukyz Zhussambayeva Gulmira Janabekova Ainash Shaimbetova Yessengali Ussenbekov Nazym Syrym |
| author_facet | Kydyrbay Maikhin Maxat Berdikulov Abdikalyk Abishov Yerlan Pazylov Gulzhan Mussayeva Slukyz Zhussambayeva Gulmira Janabekova Ainash Shaimbetova Yessengali Ussenbekov Nazym Syrym |
| author_sort | Kydyrbay Maikhin |
| collection | DOAJ |
| description | Background:
The camel pox virus is a widespread infectious viral disease of camels. It is necessary to conduct research on new strains for the development of vaccines.
Aim:
The research aims to characterize a novel strain isolated from the camel pox virus used to produce a camel pox virus vaccine.
Methods:
The objects of the study were the "M-0001" strain isolated from a sample of animals infected with the camel pox virus during the epidemic. The cultural and reproductive properties of the virus isolate were studied using primary cell lines from primary trypsinized lamb kidney and testicular cell cultures (LK and LT). Other samples included kidney cell lines from transplanted sheep) as well as a kidney cell line from transplanted cattle, Vero (transplanted green monkey kidney cell line), and calf trachea. The strain was PCR-tested and sequenced for characterization purposes.
Results:
The PCR results show that the study sample is species specific and corresponds to the camel pox virus by the size of the cumulative amplifications, which is 241 bp. Given the maximum percentage of a sequence match analyzed by the BLAST algorithm based on the international database and the results of phylogenetic analysis, the M0001 sample was determined to belong to the Camel pox virus (gene bank inventory number KP768318.1).
Conclusion:
The sample "M0001" is located on the same branch with a representative from CMLV (Camel pox virus). Among the cell cultures tested, the LK and LT cell lines were the most sensitive to the isolated CMLV isolate. Reproducing the virus in these cell cultures remains stable even after 15 consecutive passes. The cytopathic effect of the virus was less pronounced and low in transplanted cell lines, and the cytopathic effect was no longer apparent in the third passage. A genome alignment of the virus has identified potentially conserved sites, and analysis of loci in different virus types revealed one maximally conserved locus. An epizootic strain of the camelina virus "M-0001" candidate to produce vaccines for the camels was obtained. An experimental vaccine sample based on an isolated and charred camellia virus will be created in the future. [Open Vet J 2023; 13(5.000): 558-568] |
| first_indexed | 2024-03-13T02:59:13Z |
| format | Article |
| id | doaj.art-49feca5c51354fe58d1388206a79d657 |
| institution | Directory Open Access Journal |
| issn | 2226-4485 |
| language | English |
| last_indexed | 2024-03-13T02:59:13Z |
| publishDate | 2023-05-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj.art-49feca5c51354fe58d1388206a79d6572023-06-27T16:19:47ZengTripoli UniversityOpen Veterinary Journal2226-44852023-05-0113555856810.5455/OVJ.2023.v13.i5.893653Characterization of the camel pox virus strain used in producing camel pox virus vaccineKydyrbay Maikhin0Maxat Berdikulov1Abdikalyk Abishov2Yerlan Pazylov3Gulzhan Mussayeva4Slukyz Zhussambayeva5Gulmira Janabekova6Ainash Shaimbetova7Yessengali Ussenbekov8Nazym Syrym9National Reference Center for Veterinary National Reference Center for Veterinary Virology Laboratory, LLP "SPE DiaVak-ABN» National Reference Center for Veterinary National Reference Center for Veterinary National Reference Center for Veterinary Kazakh National Agrarian Research University National Reference Center for Veterinary Kazakh National Agrarian Research University Research Institute for Biological Safety ProblemsBackground: The camel pox virus is a widespread infectious viral disease of camels. It is necessary to conduct research on new strains for the development of vaccines. Aim: The research aims to characterize a novel strain isolated from the camel pox virus used to produce a camel pox virus vaccine. Methods: The objects of the study were the "M-0001" strain isolated from a sample of animals infected with the camel pox virus during the epidemic. The cultural and reproductive properties of the virus isolate were studied using primary cell lines from primary trypsinized lamb kidney and testicular cell cultures (LK and LT). Other samples included kidney cell lines from transplanted sheep) as well as a kidney cell line from transplanted cattle, Vero (transplanted green monkey kidney cell line), and calf trachea. The strain was PCR-tested and sequenced for characterization purposes. Results: The PCR results show that the study sample is species specific and corresponds to the camel pox virus by the size of the cumulative amplifications, which is 241 bp. Given the maximum percentage of a sequence match analyzed by the BLAST algorithm based on the international database and the results of phylogenetic analysis, the M0001 sample was determined to belong to the Camel pox virus (gene bank inventory number KP768318.1). Conclusion: The sample "M0001" is located on the same branch with a representative from CMLV (Camel pox virus). Among the cell cultures tested, the LK and LT cell lines were the most sensitive to the isolated CMLV isolate. Reproducing the virus in these cell cultures remains stable even after 15 consecutive passes. The cytopathic effect of the virus was less pronounced and low in transplanted cell lines, and the cytopathic effect was no longer apparent in the third passage. A genome alignment of the virus has identified potentially conserved sites, and analysis of loci in different virus types revealed one maximally conserved locus. An epizootic strain of the camelina virus "M-0001" candidate to produce vaccines for the camels was obtained. An experimental vaccine sample based on an isolated and charred camellia virus will be created in the future. [Open Vet J 2023; 13(5.000): 558-568]https://www.openveterinaryjournal.com/?mno=93653camelcamel pox virusisolateprimary and transplanted cell culture linesvirus titer |
| spellingShingle | Kydyrbay Maikhin Maxat Berdikulov Abdikalyk Abishov Yerlan Pazylov Gulzhan Mussayeva Slukyz Zhussambayeva Gulmira Janabekova Ainash Shaimbetova Yessengali Ussenbekov Nazym Syrym Characterization of the camel pox virus strain used in producing camel pox virus vaccine Open Veterinary Journal camel camel pox virus isolate primary and transplanted cell culture lines virus titer |
| title | Characterization of the camel pox virus strain used in producing camel pox virus vaccine |
| title_full | Characterization of the camel pox virus strain used in producing camel pox virus vaccine |
| title_fullStr | Characterization of the camel pox virus strain used in producing camel pox virus vaccine |
| title_full_unstemmed | Characterization of the camel pox virus strain used in producing camel pox virus vaccine |
| title_short | Characterization of the camel pox virus strain used in producing camel pox virus vaccine |
| title_sort | characterization of the camel pox virus strain used in producing camel pox virus vaccine |
| topic | camel camel pox virus isolate primary and transplanted cell culture lines virus titer |
| url | https://www.openveterinaryjournal.com/?mno=93653 |
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