Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickens
E. coli infections in avian species are an economic threat to the poultry industry worldwide. The spread of MDR bacteria has been recognized as an increasing problem in the veterinary and medical fields. The current study aimed to investigate the phenotypic and genotypic antimicrobial patterns of av...
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| Format: | Article |
| Language: | English |
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Faculty of Veterinary Medicine, Beni-Suef University
2019-06-01
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| Series: | Journal of Veterinary Medical Research |
| Subjects: | |
| Online Access: | http://www.bsu.edu.eg/bsujournals/Content.aspx?paperId=554&cat_id=1 |
| _version_ | 1818918647631970304 |
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| author | Ismail A. Radwan Sabry M. Tamam Abeer A. El Sayed Rania O. Qurani |
| author_facet | Ismail A. Radwan Sabry M. Tamam Abeer A. El Sayed Rania O. Qurani |
| author_sort | Ismail A. Radwan |
| collection | DOAJ |
| description | E. coli infections in avian species are an economic threat to the poultry industry worldwide. The spread of MDR bacteria has been recognized as an increasing problem in the veterinary and medical fields. The current study aimed to investigate the phenotypic and genotypic antimicrobial patterns of avian Pathogenic E. coli isolated from broiler chickens. Results of in-vitro antimicrobial susceptibility testing showed that E. coli isolates were more sensitive to imipenem only (72.4%). On the other hand, they were highly resistant to most of used antimicrobials including ciprofloxacin (95.9%), amikacin (94.9%), cefotaxime sodium (92.9%), gentamicin (89.9%), cefotriaxone (89.9%), topramycin (87.8%), sulphamethoxazole/trimethoprime (85.7%), ceftazidim (84.7%). Also, they were resistant to cefoprazone (79.9%), doxycycline (72.4%) and amoxycillin/clavulinic acid (69.4%). All the tested isolates of E. coli (100%) were multi drug resistant (MDR). PCR was applied on 15 MDR E. coli isolates to determine 4 genes responsible for antibiotic resistance included ampC, blaCTX, blaSHV and tetA (A). The results revealed that that ampC and blaCTX genes were the most prevalent found in all isolates (100%) while tetA (A) and blaSHV genes were harbored in 14 isolates (93.3%). |
| first_indexed | 2024-12-20T00:53:17Z |
| format | Article |
| id | doaj.art-49ffb0219eeb4c108e8ee667fbe51d63 |
| institution | Directory Open Access Journal |
| issn | 2357-0512 2357-0520 |
| language | English |
| last_indexed | 2024-12-20T00:53:17Z |
| publishDate | 2019-06-01 |
| publisher | Faculty of Veterinary Medicine, Beni-Suef University |
| record_format | Article |
| series | Journal of Veterinary Medical Research |
| spelling | doaj.art-49ffb0219eeb4c108e8ee667fbe51d632022-12-21T19:59:10ZengFaculty of Veterinary Medicine, Beni-Suef UniversityJournal of Veterinary Medical Research2357-05122357-05202019-06-01261134142Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickensIsmail A. Radwan0Sabry M. TamamAbeer A. El Sayed1Rania O. Qurani2Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.Bacteriology, Mycology and Immunology Department, Faculty of Veterinary Medicine, Beni-Suef University, Egypt.E. coli infections in avian species are an economic threat to the poultry industry worldwide. The spread of MDR bacteria has been recognized as an increasing problem in the veterinary and medical fields. The current study aimed to investigate the phenotypic and genotypic antimicrobial patterns of avian Pathogenic E. coli isolated from broiler chickens. Results of in-vitro antimicrobial susceptibility testing showed that E. coli isolates were more sensitive to imipenem only (72.4%). On the other hand, they were highly resistant to most of used antimicrobials including ciprofloxacin (95.9%), amikacin (94.9%), cefotaxime sodium (92.9%), gentamicin (89.9%), cefotriaxone (89.9%), topramycin (87.8%), sulphamethoxazole/trimethoprime (85.7%), ceftazidim (84.7%). Also, they were resistant to cefoprazone (79.9%), doxycycline (72.4%) and amoxycillin/clavulinic acid (69.4%). All the tested isolates of E. coli (100%) were multi drug resistant (MDR). PCR was applied on 15 MDR E. coli isolates to determine 4 genes responsible for antibiotic resistance included ampC, blaCTX, blaSHV and tetA (A). The results revealed that that ampC and blaCTX genes were the most prevalent found in all isolates (100%) while tetA (A) and blaSHV genes were harbored in 14 isolates (93.3%).http://www.bsu.edu.eg/bsujournals/Content.aspx?paperId=554&cat_id=1E. colibroiler chickensresistance genes |
| spellingShingle | Ismail A. Radwan Sabry M. Tamam Abeer A. El Sayed Rania O. Qurani Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickens Journal of Veterinary Medical Research E. coli broiler chickens resistance genes |
| title | Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickens |
| title_full | Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickens |
| title_fullStr | Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickens |
| title_full_unstemmed | Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickens |
| title_short | Antimicrobial patterns of Avian Pathogenic Escherichia coli isolated from broiler chickens |
| title_sort | antimicrobial patterns of avian pathogenic escherichia coli isolated from broiler chickens |
| topic | E. coli broiler chickens resistance genes |
| url | http://www.bsu.edu.eg/bsujournals/Content.aspx?paperId=554&cat_id=1 |
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