Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY.
The cascade of molecular events involved in mammalian sex determination has been shown to involve the SRY gene, but specific downstream events have eluded researchers for decades. The current study identifies one of the first direct downstream targets of the male sex determining factor SRY as the ba...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3101584?pdf=render |
| _version_ | 1811202290125635584 |
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| author | Ramji K Bhandari Ingrid Sadler-Riggleman Tracy M Clement Michael K Skinner |
| author_facet | Ramji K Bhandari Ingrid Sadler-Riggleman Tracy M Clement Michael K Skinner |
| author_sort | Ramji K Bhandari |
| collection | DOAJ |
| description | The cascade of molecular events involved in mammalian sex determination has been shown to involve the SRY gene, but specific downstream events have eluded researchers for decades. The current study identifies one of the first direct downstream targets of the male sex determining factor SRY as the basic-helix-loop-helix (bHLH) transcription factor TCF21. SRY was found to bind to the Tcf21 promoter and activate gene expression. Mutagenesis of SRY/SOX9 response elements in the Tcf21 promoter eliminated the actions of SRY. SRY was found to directly associate with the Tcf21 promoter SRY/SOX9 response elements in vivo during fetal rat testis development. TCF21 was found to promote an in vitro sex reversal of embryonic ovarian cells to induce precursor Sertoli cell differentiation. TCF21 and SRY had similar effects on the in vitro sex reversal gonadal cell transcriptomes. Therefore, SRY acts directly on the Tcf21 promoter to in part initiate a cascade of events associated with Sertoli cell differentiation and embryonic testis development. |
| first_indexed | 2024-04-12T02:36:19Z |
| format | Article |
| id | doaj.art-4a00e49ea37343f6b28ae5d5981c1607 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-12T02:36:19Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4a00e49ea37343f6b28ae5d5981c16072022-12-22T03:51:31ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-0165e1993510.1371/journal.pone.0019935Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY.Ramji K BhandariIngrid Sadler-RigglemanTracy M ClementMichael K SkinnerThe cascade of molecular events involved in mammalian sex determination has been shown to involve the SRY gene, but specific downstream events have eluded researchers for decades. The current study identifies one of the first direct downstream targets of the male sex determining factor SRY as the basic-helix-loop-helix (bHLH) transcription factor TCF21. SRY was found to bind to the Tcf21 promoter and activate gene expression. Mutagenesis of SRY/SOX9 response elements in the Tcf21 promoter eliminated the actions of SRY. SRY was found to directly associate with the Tcf21 promoter SRY/SOX9 response elements in vivo during fetal rat testis development. TCF21 was found to promote an in vitro sex reversal of embryonic ovarian cells to induce precursor Sertoli cell differentiation. TCF21 and SRY had similar effects on the in vitro sex reversal gonadal cell transcriptomes. Therefore, SRY acts directly on the Tcf21 promoter to in part initiate a cascade of events associated with Sertoli cell differentiation and embryonic testis development.http://europepmc.org/articles/PMC3101584?pdf=render |
| spellingShingle | Ramji K Bhandari Ingrid Sadler-Riggleman Tracy M Clement Michael K Skinner Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY. PLoS ONE |
| title | Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY. |
| title_full | Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY. |
| title_fullStr | Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY. |
| title_full_unstemmed | Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY. |
| title_short | Basic helix-loop-helix transcription factor TCF21 is a downstream target of the male sex determining gene SRY. |
| title_sort | basic helix loop helix transcription factor tcf21 is a downstream target of the male sex determining gene sry |
| url | http://europepmc.org/articles/PMC3101584?pdf=render |
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