Gene flow in a Yersinia pestis vector, Oropsylla hirsuta, during a plague epizootic
Background & objectives: Appreciating how Yersinia pestis, the etiological agent of plague, spreads amongblack-tailed prairie dog (Cynomys ludovicianus) colonies (BTPD), is vital to wildlife conservation programsin North American grasslands. A little-studied aspect of the system is the role of Y...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2011-09-01
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| Series: | Journal of Vector Borne Diseases |
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| Online Access: | http://www.mrcindia.org/journal/issues/483125.pdf |
| _version_ | 1819120511903334400 |
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| author | Philip H. Jones , Leigh R. Washburn & Hugh B. Britten |
| author_facet | Philip H. Jones , Leigh R. Washburn & Hugh B. Britten |
| author_sort | Philip H. Jones , Leigh R. Washburn & Hugh B. Britten |
| collection | DOAJ |
| description | Background & objectives: Appreciating how Yersinia pestis, the etiological agent of plague, spreads amongblack-tailed prairie dog (Cynomys ludovicianus) colonies (BTPD), is vital to wildlife conservation programsin North American grasslands. A little-studied aspect of the system is the role of Y. pestis vectors, i.e. fleas,play in the spreading of plague in natural settings. We investigated the genetic structure and variability of acommon prairie dog flea (Oropsylla hirsuta) in BTPD colonies in order to examine dispersal patterns. Giventhat this research took place during a widespread plague epizootic, there was the added advantage of gaininginformation on the dynamics of sylvatic plague.Methods & Results: Oropsylla hirsuta were collected from BTPD burrows in nine colonies from May 2005 toJuly 2005, and eight polymorphic microsatellite markers were used to generate genotypic data from them.Gene flow estimates revealed low genetic differentiation among fleas sampled from different colonies. NestedPCR plague assays confirmed the presence of Y. pestis with the average Y. pestis prevalence across all ninecolonies at 12%. No significant correlations were found between the genetic variability and gene flow of O.hirsuta and Y. pestis prevalence on a per-colony basis.Conclusion: Oropsylla hirsuta dispersal among BTPD colonies was high, potentially explaining the rapidspread of Y. pestis in our study area in 2005 and 2006. |
| first_indexed | 2024-12-22T06:21:50Z |
| format | Article |
| id | doaj.art-4a05f796d0334f3bafa6eb710d9d0222 |
| institution | Directory Open Access Journal |
| issn | 0972-9062 |
| language | English |
| last_indexed | 2024-12-22T06:21:50Z |
| publishDate | 2011-09-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Vector Borne Diseases |
| spelling | doaj.art-4a05f796d0334f3bafa6eb710d9d02222022-12-21T18:35:57ZengWolters Kluwer Medknow PublicationsJournal of Vector Borne Diseases0972-90622011-09-01483125132Gene flow in a Yersinia pestis vector, Oropsylla hirsuta, during a plague epizooticPhilip H. Jones , Leigh R. Washburn & Hugh B. BrittenBackground & objectives: Appreciating how Yersinia pestis, the etiological agent of plague, spreads amongblack-tailed prairie dog (Cynomys ludovicianus) colonies (BTPD), is vital to wildlife conservation programsin North American grasslands. A little-studied aspect of the system is the role of Y. pestis vectors, i.e. fleas,play in the spreading of plague in natural settings. We investigated the genetic structure and variability of acommon prairie dog flea (Oropsylla hirsuta) in BTPD colonies in order to examine dispersal patterns. Giventhat this research took place during a widespread plague epizootic, there was the added advantage of gaininginformation on the dynamics of sylvatic plague.Methods & Results: Oropsylla hirsuta were collected from BTPD burrows in nine colonies from May 2005 toJuly 2005, and eight polymorphic microsatellite markers were used to generate genotypic data from them.Gene flow estimates revealed low genetic differentiation among fleas sampled from different colonies. NestedPCR plague assays confirmed the presence of Y. pestis with the average Y. pestis prevalence across all ninecolonies at 12%. No significant correlations were found between the genetic variability and gene flow of O.hirsuta and Y. pestis prevalence on a per-colony basis.Conclusion: Oropsylla hirsuta dispersal among BTPD colonies was high, potentially explaining the rapidspread of Y. pestis in our study area in 2005 and 2006.http://www.mrcindia.org/journal/issues/483125.pdfDispersalfleamicrosatellitesPCRprairie dogsylvatic plagu |
| spellingShingle | Philip H. Jones , Leigh R. Washburn & Hugh B. Britten Gene flow in a Yersinia pestis vector, Oropsylla hirsuta, during a plague epizootic Journal of Vector Borne Diseases Dispersal flea microsatellites PCR prairie dog sylvatic plagu |
| title | Gene flow in a Yersinia pestis vector, Oropsylla hirsuta, during a plague epizootic |
| title_full | Gene flow in a Yersinia pestis vector, Oropsylla hirsuta, during a plague epizootic |
| title_fullStr | Gene flow in a Yersinia pestis vector, Oropsylla hirsuta, during a plague epizootic |
| title_full_unstemmed | Gene flow in a Yersinia pestis vector, Oropsylla hirsuta, during a plague epizootic |
| title_short | Gene flow in a Yersinia pestis vector, Oropsylla hirsuta, during a plague epizootic |
| title_sort | gene flow in a yersinia pestis vector oropsylla hirsuta during a plague epizootic |
| topic | Dispersal flea microsatellites PCR prairie dog sylvatic plagu |
| url | http://www.mrcindia.org/journal/issues/483125.pdf |
| work_keys_str_mv | AT philiphjonesleighrwashburnhughbbritten geneflowinayersiniapestisvectororopsyllahirsutaduringaplagueepizootic |