Reference values for thrombin dynamics in platelet rich plasma
Thrombin generation (TG) is a better determinant of the overall function of the hemostatic system than routinely used clotting time-based assays and can be studied more in detail by thrombin dynamics analysis. Platelet poor plasma is often used to measure TG, however, measuring the contribution of t...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-02-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2020.1742310 |
| _version_ | 1797684195675340800 |
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| author | Qiuting Yan Marisa Ninivaggi Bas de Laat Romy M. W. de Laat-Kremers |
| author_facet | Qiuting Yan Marisa Ninivaggi Bas de Laat Romy M. W. de Laat-Kremers |
| author_sort | Qiuting Yan |
| collection | DOAJ |
| description | Thrombin generation (TG) is a better determinant of the overall function of the hemostatic system than routinely used clotting time-based assays and can be studied more in detail by thrombin dynamics analysis. Platelet poor plasma is often used to measure TG, however, measuring the contribution of the platelets is also important as patients with a low platelet count or with dysfunctional platelets have an increased risk of developing bleeding. In this study, platelet rich plasma (PRP) was collected from 117 healthy individuals. PRP was measured undiluted and diluted to a varying platelet concentration of 10*109/L to 400*109/L. Prothrombin conversion and thrombin inactivation were calculated from the data obtained by the TG parameters and coagulation factor levels (antithrombin, α2Macroglobulin (α2M) and fibrinogen). Reference ranges of TG and thrombin dynamics in PRP of 117 healthy individuals were established. Peak, velocity index and the maximum rate of prothrombin conversion increased linearly with platelet count, but endogenous thrombin potential reached a maximum at 150*109/L as seen in a subset population (n = 20). More extensive analysis revealed that a platelet count below 50*109/L did not affect TG parameters (except for the ETP). Correlation analysis indicated that the platelet count mainly affected the rate of prothrombin conversion. Inhibition of thrombin by antithrombin and α2M increased with increasing TG, but the ratio of inhibition by antithrombin or α2M remained the same independently of the total thrombin formed. In conclusion, TG and thrombin dynamics were assessed in PRP of healthy donors to provide reference values for future TG studies in PRP. Increasing the platelet count mainly affected the rate of prothrombin conversion and TG, rather than the total amount of thrombin formed. |
| first_indexed | 2024-03-12T00:26:07Z |
| format | Article |
| id | doaj.art-4a06d870e91b4382a20554c3750e217c |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:26:07Z |
| publishDate | 2021-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-4a06d870e91b4382a20554c3750e217c2023-09-15T10:38:07ZengTaylor & Francis GroupPlatelets0953-71041369-16352021-02-0132225125810.1080/09537104.2020.17423101742310Reference values for thrombin dynamics in platelet rich plasmaQiuting Yan0Marisa Ninivaggi1Bas de Laat2Romy M. W. de Laat-Kremers3Synapse Research InstituteSynapse Research InstituteSynapse Research InstituteCARIM, Maastricht UniversityThrombin generation (TG) is a better determinant of the overall function of the hemostatic system than routinely used clotting time-based assays and can be studied more in detail by thrombin dynamics analysis. Platelet poor plasma is often used to measure TG, however, measuring the contribution of the platelets is also important as patients with a low platelet count or with dysfunctional platelets have an increased risk of developing bleeding. In this study, platelet rich plasma (PRP) was collected from 117 healthy individuals. PRP was measured undiluted and diluted to a varying platelet concentration of 10*109/L to 400*109/L. Prothrombin conversion and thrombin inactivation were calculated from the data obtained by the TG parameters and coagulation factor levels (antithrombin, α2Macroglobulin (α2M) and fibrinogen). Reference ranges of TG and thrombin dynamics in PRP of 117 healthy individuals were established. Peak, velocity index and the maximum rate of prothrombin conversion increased linearly with platelet count, but endogenous thrombin potential reached a maximum at 150*109/L as seen in a subset population (n = 20). More extensive analysis revealed that a platelet count below 50*109/L did not affect TG parameters (except for the ETP). Correlation analysis indicated that the platelet count mainly affected the rate of prothrombin conversion. Inhibition of thrombin by antithrombin and α2M increased with increasing TG, but the ratio of inhibition by antithrombin or α2M remained the same independently of the total thrombin formed. In conclusion, TG and thrombin dynamics were assessed in PRP of healthy donors to provide reference values for future TG studies in PRP. Increasing the platelet count mainly affected the rate of prothrombin conversion and TG, rather than the total amount of thrombin formed.http://dx.doi.org/10.1080/09537104.2020.1742310platelet rich plasmaprothrombin conversionthrombin dynamicsthrombin generationthrombin inactivation |
| spellingShingle | Qiuting Yan Marisa Ninivaggi Bas de Laat Romy M. W. de Laat-Kremers Reference values for thrombin dynamics in platelet rich plasma Platelets platelet rich plasma prothrombin conversion thrombin dynamics thrombin generation thrombin inactivation |
| title | Reference values for thrombin dynamics in platelet rich plasma |
| title_full | Reference values for thrombin dynamics in platelet rich plasma |
| title_fullStr | Reference values for thrombin dynamics in platelet rich plasma |
| title_full_unstemmed | Reference values for thrombin dynamics in platelet rich plasma |
| title_short | Reference values for thrombin dynamics in platelet rich plasma |
| title_sort | reference values for thrombin dynamics in platelet rich plasma |
| topic | platelet rich plasma prothrombin conversion thrombin dynamics thrombin generation thrombin inactivation |
| url | http://dx.doi.org/10.1080/09537104.2020.1742310 |
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