<it>Corky</it>, a <it>gypsy</it>-like retrotransposon is differentially transcribed in <it>Quercus suber</it> tissues
<p>Abstract</p> <p>Background</p> <p>Transposable elements (TEs) make up a large part of eukaryotic genomes. Due to their repetitive nature and to the fact that they harbour regulatory signals, TEs can be responsible for chromosomal rearrangements, movement of gene sequ...
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| Format: | Article |
| Language: | English |
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BMC
2012-08-01
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| Series: | BMC Research Notes |
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| Online Access: | http://www.biomedcentral.com/1756-0500/5/432 |
| _version_ | 1811278781578477568 |
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| author | Rocheta Margarida Carvalho Luísa Viegas Wanda Morais-Cecílio Leonor |
| author_facet | Rocheta Margarida Carvalho Luísa Viegas Wanda Morais-Cecílio Leonor |
| author_sort | Rocheta Margarida |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Transposable elements (TEs) make up a large part of eukaryotic genomes. Due to their repetitive nature and to the fact that they harbour regulatory signals, TEs can be responsible for chromosomal rearrangements, movement of gene sequences and evolution of gene regulation and function. Retrotransposon ubiquity raises the question about their function in genomes and most are transcriptionally inactive due to rearrangements that compromise their activity. However, the activity of TEs is currently considered to have been one of the major processes in genome evolution.</p> <p>Findings</p> <p>We report on the characterization of a transcriptionally active <it>gypsy</it>-like retrotransposon (named <it>Corky</it>) from <it>Quercus suber</it>, in a comparative and quantitative study of expression levels in different tissues and distinct developmental stages through RT-qPCR. We observed <it>Corky</it>’s differential transcription levels in all the tissues analysed.</p> <p>Conclusions</p> <p>These results document that <it>Corky</it>’s transcription levels are not constant. Nevertheless, they depend upon the developmental stage, the tissue analysed and the potential occurring events during an individuals’ life span. This modulation brought upon by different developmental and environmental influences suggests an involvement of <it>Corky</it> in stress response and during development.</p> |
| first_indexed | 2024-04-13T00:43:07Z |
| format | Article |
| id | doaj.art-4a079ef2182342d7ba3fd1169fd80dea |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-04-13T00:43:07Z |
| publishDate | 2012-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-4a079ef2182342d7ba3fd1169fd80dea2022-12-22T03:10:06ZengBMCBMC Research Notes1756-05002012-08-015143210.1186/1756-0500-5-432<it>Corky</it>, a <it>gypsy</it>-like retrotransposon is differentially transcribed in <it>Quercus suber</it> tissuesRocheta MargaridaCarvalho LuísaViegas WandaMorais-Cecílio Leonor<p>Abstract</p> <p>Background</p> <p>Transposable elements (TEs) make up a large part of eukaryotic genomes. Due to their repetitive nature and to the fact that they harbour regulatory signals, TEs can be responsible for chromosomal rearrangements, movement of gene sequences and evolution of gene regulation and function. Retrotransposon ubiquity raises the question about their function in genomes and most are transcriptionally inactive due to rearrangements that compromise their activity. However, the activity of TEs is currently considered to have been one of the major processes in genome evolution.</p> <p>Findings</p> <p>We report on the characterization of a transcriptionally active <it>gypsy</it>-like retrotransposon (named <it>Corky</it>) from <it>Quercus suber</it>, in a comparative and quantitative study of expression levels in different tissues and distinct developmental stages through RT-qPCR. We observed <it>Corky</it>’s differential transcription levels in all the tissues analysed.</p> <p>Conclusions</p> <p>These results document that <it>Corky</it>’s transcription levels are not constant. Nevertheless, they depend upon the developmental stage, the tissue analysed and the potential occurring events during an individuals’ life span. This modulation brought upon by different developmental and environmental influences suggests an involvement of <it>Corky</it> in stress response and during development.</p>http://www.biomedcentral.com/1756-0500/5/432<it>Quercus suber</it><it>Corky</it>LTR retroelementRT-qPCRExpression |
| spellingShingle | Rocheta Margarida Carvalho Luísa Viegas Wanda Morais-Cecílio Leonor <it>Corky</it>, a <it>gypsy</it>-like retrotransposon is differentially transcribed in <it>Quercus suber</it> tissues BMC Research Notes <it>Quercus suber</it> <it>Corky</it> LTR retroelement RT-qPCR Expression |
| title | <it>Corky</it>, a <it>gypsy</it>-like retrotransposon is differentially transcribed in <it>Quercus suber</it> tissues |
| title_full | <it>Corky</it>, a <it>gypsy</it>-like retrotransposon is differentially transcribed in <it>Quercus suber</it> tissues |
| title_fullStr | <it>Corky</it>, a <it>gypsy</it>-like retrotransposon is differentially transcribed in <it>Quercus suber</it> tissues |
| title_full_unstemmed | <it>Corky</it>, a <it>gypsy</it>-like retrotransposon is differentially transcribed in <it>Quercus suber</it> tissues |
| title_short | <it>Corky</it>, a <it>gypsy</it>-like retrotransposon is differentially transcribed in <it>Quercus suber</it> tissues |
| title_sort | it corky it a it gypsy it like retrotransposon is differentially transcribed in it quercus suber it tissues |
| topic | <it>Quercus suber</it> <it>Corky</it> LTR retroelement RT-qPCR Expression |
| url | http://www.biomedcentral.com/1756-0500/5/432 |
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