Evaluation of polymerase chain reaction using primer MPB 64 for diagnosis of clinically suspected cases of extrapulmonary tuberculosis
Background: Pulmonary tuberculosis can be easily diagnosed by simple techniques such as microscopy. However, extrapulmonary tuberculosis (EPTB) often presents a diagnostic dilemma. Microscopy and culture have proved to be insensitive techniques for diagnosis of EPTB. There is an urgent need for rapi...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Journal of the Scientific Society |
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| Online Access: | http://www.jscisociety.com/article.asp?issn=0974-5009;year=2014;volume=41;issue=2;spage=89;epage=93;aulast=Ghadage |
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| author | Dnyaneshwari P. Ghadage Vrishali A. Muley Sushma Pednekar Arvind V. Bhore |
| author_facet | Dnyaneshwari P. Ghadage Vrishali A. Muley Sushma Pednekar Arvind V. Bhore |
| author_sort | Dnyaneshwari P. Ghadage |
| collection | DOAJ |
| description | Background: Pulmonary tuberculosis can be easily diagnosed by simple techniques such as microscopy. However, extrapulmonary tuberculosis (EPTB) often presents a diagnostic dilemma. Microscopy and culture have proved to be insensitive techniques for diagnosis of EPTB. There is an urgent need for rapid and sensitive diagnostic methods. Aim: The present study was conducted to evaluate the role of polymerase chain reaction (PCR) in the early diagnosis of clinically suspected cases of EPTB. Materials and Methods: A total of 80 clinical specimens comprising pleural fluid, cerebrospinal fluid, ascitic fluid, fine-needle aspiration biopsy, and pus and biopsy from clinically suspected EPTB cases were processed and followed up by conventional methods and PCR using MPB64 primer. Results: Tuberculous pleural effusion (71%) was found to be the most common clinical presentation of EPTB. Overall, PCR could detect EPTB in 61.2% cases. Microscopy and culture could detect 18.7% and 22.5% EPTB cases, respectively. PCR was positive in all tissue samples suggestive of tuberculosis on histopathological examination. Of the 62 EPTB patients who responded to antituberculosis treatment (ATT), 49 patients were PCR positive. Conclusion: PCR using MPB64 had a significant advantage over the conventional methods to detect the presence of M. tuberculosis in specimens of clinically suspected EPTB patients for early diagnosis of tuberculosis. |
| first_indexed | 2024-12-20T04:04:37Z |
| format | Article |
| id | doaj.art-4a0a8f9109af4764b09390a728621e3c |
| institution | Directory Open Access Journal |
| issn | 0974-5009 |
| language | English |
| last_indexed | 2024-12-20T04:04:37Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of the Scientific Society |
| spelling | doaj.art-4a0a8f9109af4764b09390a728621e3c2022-12-21T19:54:04ZengWolters Kluwer Medknow PublicationsJournal of the Scientific Society0974-50092014-01-01412899310.4103/0974-5009.132837Evaluation of polymerase chain reaction using primer MPB 64 for diagnosis of clinically suspected cases of extrapulmonary tuberculosisDnyaneshwari P. GhadageVrishali A. MuleySushma PednekarArvind V. BhoreBackground: Pulmonary tuberculosis can be easily diagnosed by simple techniques such as microscopy. However, extrapulmonary tuberculosis (EPTB) often presents a diagnostic dilemma. Microscopy and culture have proved to be insensitive techniques for diagnosis of EPTB. There is an urgent need for rapid and sensitive diagnostic methods. Aim: The present study was conducted to evaluate the role of polymerase chain reaction (PCR) in the early diagnosis of clinically suspected cases of EPTB. Materials and Methods: A total of 80 clinical specimens comprising pleural fluid, cerebrospinal fluid, ascitic fluid, fine-needle aspiration biopsy, and pus and biopsy from clinically suspected EPTB cases were processed and followed up by conventional methods and PCR using MPB64 primer. Results: Tuberculous pleural effusion (71%) was found to be the most common clinical presentation of EPTB. Overall, PCR could detect EPTB in 61.2% cases. Microscopy and culture could detect 18.7% and 22.5% EPTB cases, respectively. PCR was positive in all tissue samples suggestive of tuberculosis on histopathological examination. Of the 62 EPTB patients who responded to antituberculosis treatment (ATT), 49 patients were PCR positive. Conclusion: PCR using MPB64 had a significant advantage over the conventional methods to detect the presence of M. tuberculosis in specimens of clinically suspected EPTB patients for early diagnosis of tuberculosis.http://www.jscisociety.com/article.asp?issn=0974-5009;year=2014;volume=41;issue=2;spage=89;epage=93;aulast=GhadageConventional techniquesextrapulmonary tuberculosis (EPTB)polymerase chain reaction (PCR) |
| spellingShingle | Dnyaneshwari P. Ghadage Vrishali A. Muley Sushma Pednekar Arvind V. Bhore Evaluation of polymerase chain reaction using primer MPB 64 for diagnosis of clinically suspected cases of extrapulmonary tuberculosis Journal of the Scientific Society Conventional techniques extrapulmonary tuberculosis (EPTB) polymerase chain reaction (PCR) |
| title | Evaluation of polymerase chain reaction using primer MPB 64 for diagnosis of clinically suspected cases of extrapulmonary tuberculosis |
| title_full | Evaluation of polymerase chain reaction using primer MPB 64 for diagnosis of clinically suspected cases of extrapulmonary tuberculosis |
| title_fullStr | Evaluation of polymerase chain reaction using primer MPB 64 for diagnosis of clinically suspected cases of extrapulmonary tuberculosis |
| title_full_unstemmed | Evaluation of polymerase chain reaction using primer MPB 64 for diagnosis of clinically suspected cases of extrapulmonary tuberculosis |
| title_short | Evaluation of polymerase chain reaction using primer MPB 64 for diagnosis of clinically suspected cases of extrapulmonary tuberculosis |
| title_sort | evaluation of polymerase chain reaction using primer mpb 64 for diagnosis of clinically suspected cases of extrapulmonary tuberculosis |
| topic | Conventional techniques extrapulmonary tuberculosis (EPTB) polymerase chain reaction (PCR) |
| url | http://www.jscisociety.com/article.asp?issn=0974-5009;year=2014;volume=41;issue=2;spage=89;epage=93;aulast=Ghadage |
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