Mass Spectrometry-Based Proteomic Profiling of a Silvaner White Wine
The comprehensive identification of the proteome content from a white wine (cv. Silvaner) is described here for the first time. The wine protein composition isolated from a representative wine sample (250 L) was identified via mass spectrometry (MS)-based proteomics following <i>in-solution<...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/4/650 |
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| author | Wendell Albuquerque Parviz Ghezellou Leif Seidel Johannes Burkert Frank Will Ralf Schweiggert Bernhard Spengler Holger Zorn Martin Gand |
| author_facet | Wendell Albuquerque Parviz Ghezellou Leif Seidel Johannes Burkert Frank Will Ralf Schweiggert Bernhard Spengler Holger Zorn Martin Gand |
| author_sort | Wendell Albuquerque |
| collection | DOAJ |
| description | The comprehensive identification of the proteome content from a white wine (cv. Silvaner) is described here for the first time. The wine protein composition isolated from a representative wine sample (250 L) was identified via mass spectrometry (MS)-based proteomics following <i>in-solution</i> and <i>in-gel</i> digestion methods after being submitted to size exclusion chromatographic (SEC) fractionation to gain a comprehensive insight into proteins that survive the vinification processes. In total, we identified 154 characterized (with described functional information) or so far uncharacterized proteins, mainly from <i>Vitis vinifera</i> L. and <i>Saccharomyces cerevisiae</i>. With the complementarity of the two-step purification, the digestion techniques and the high-resolution (HR)-MS analyses provided a high-score identification of proteins from low to high abundance. These proteins can be valuable for future authentication of wines by tracing proteins derived from a specific cultivar or winemaking process. The proteomics approach presented herein may also be generally helpful to understand which proteins are important for the organoleptic properties and stability of wines. |
| first_indexed | 2024-03-11T05:11:54Z |
| format | Article |
| id | doaj.art-4a0b6049d524461987831db74d413891 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-11T05:11:54Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-4a0b6049d524461987831db74d4138912023-11-17T18:29:26ZengMDPI AGBiomolecules2218-273X2023-04-0113465010.3390/biom13040650Mass Spectrometry-Based Proteomic Profiling of a Silvaner White WineWendell Albuquerque0Parviz Ghezellou1Leif Seidel2Johannes Burkert3Frank Will4Ralf Schweiggert5Bernhard Spengler6Holger Zorn7Martin Gand8Institute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyInstitute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyDepartment of Beverage Research, Geisenheim University, Von-Lade-Strasse 1, 65366 Geisenheim, GermanyInstitute for Viticulture and Oenology, Bavarian State Institute for Viticulture and Horticulture (LWG), An der Steige 15, 97209 Veitshöchheim, GermanyDepartment of Beverage Research, Geisenheim University, Von-Lade-Strasse 1, 65366 Geisenheim, GermanyDepartment of Beverage Research, Geisenheim University, Von-Lade-Strasse 1, 65366 Geisenheim, GermanyInstitute of Inorganic and Analytical Chemistry, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyInstitute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyInstitute of Food Chemistry and Food Biotechnology, Justus Liebig University Giessen, Heinrich-Buff-Ring 17, 35392 Giessen, GermanyThe comprehensive identification of the proteome content from a white wine (cv. Silvaner) is described here for the first time. The wine protein composition isolated from a representative wine sample (250 L) was identified via mass spectrometry (MS)-based proteomics following <i>in-solution</i> and <i>in-gel</i> digestion methods after being submitted to size exclusion chromatographic (SEC) fractionation to gain a comprehensive insight into proteins that survive the vinification processes. In total, we identified 154 characterized (with described functional information) or so far uncharacterized proteins, mainly from <i>Vitis vinifera</i> L. and <i>Saccharomyces cerevisiae</i>. With the complementarity of the two-step purification, the digestion techniques and the high-resolution (HR)-MS analyses provided a high-score identification of proteins from low to high abundance. These proteins can be valuable for future authentication of wines by tracing proteins derived from a specific cultivar or winemaking process. The proteomics approach presented herein may also be generally helpful to understand which proteins are important for the organoleptic properties and stability of wines.https://www.mdpi.com/2218-273X/13/4/650Silvanerproteomicswineproteinsmass spectrometry<i>Vitis vinifera</i> |
| spellingShingle | Wendell Albuquerque Parviz Ghezellou Leif Seidel Johannes Burkert Frank Will Ralf Schweiggert Bernhard Spengler Holger Zorn Martin Gand Mass Spectrometry-Based Proteomic Profiling of a Silvaner White Wine Biomolecules Silvaner proteomics wine proteins mass spectrometry <i>Vitis vinifera</i> |
| title | Mass Spectrometry-Based Proteomic Profiling of a Silvaner White Wine |
| title_full | Mass Spectrometry-Based Proteomic Profiling of a Silvaner White Wine |
| title_fullStr | Mass Spectrometry-Based Proteomic Profiling of a Silvaner White Wine |
| title_full_unstemmed | Mass Spectrometry-Based Proteomic Profiling of a Silvaner White Wine |
| title_short | Mass Spectrometry-Based Proteomic Profiling of a Silvaner White Wine |
| title_sort | mass spectrometry based proteomic profiling of a silvaner white wine |
| topic | Silvaner proteomics wine proteins mass spectrometry <i>Vitis vinifera</i> |
| url | https://www.mdpi.com/2218-273X/13/4/650 |
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