Caspase inhibition improves viability and efficiency of liposomal transfection
Abstract High transfection efficiency is the most important point for experiments of DNA and RNA introduction into cells. Decrease of cell viability during the transfection procedure is a crucial issue, resulting in transfection failure. However, the mechanism underlying cell growth inhibition has n...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-12-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-49027-y |
| _version_ | 1797398005574270976 |
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| author | Tatsushi Yoshida Kenta Yamasaki Kenjiro Tadagaki |
| author_facet | Tatsushi Yoshida Kenta Yamasaki Kenjiro Tadagaki |
| author_sort | Tatsushi Yoshida |
| collection | DOAJ |
| description | Abstract High transfection efficiency is the most important point for experiments of DNA and RNA introduction into cells. Decrease of cell viability during the transfection procedure is a crucial issue, resulting in transfection failure. However, the mechanism underlying cell growth inhibition has not been fully elucidated. Lipofection is frequently used for transfection experiments, whereases, depending on cell type, it causes a decrease in cell viability. The present study demonstrates here that a potent pan-caspase inhibitor Q-VD-OPh blocked cell death during the lipofection, indicating apoptosis was induced in lipofection. Moreover, Q-VD-OPh drastically increased transfected cells. This method provides easier and more effective transfection system of lipofection and may be useful for transfection of not only cell lines but also clinical uses such as gene therapy and nucleic acids vaccine. |
| first_indexed | 2024-03-09T01:18:28Z |
| format | Article |
| id | doaj.art-4a0c471400274bf5986b51642f0702ab |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T01:18:28Z |
| publishDate | 2023-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4a0c471400274bf5986b51642f0702ab2023-12-10T12:17:15ZengNature PortfolioScientific Reports2045-23222023-12-011311910.1038/s41598-023-49027-yCaspase inhibition improves viability and efficiency of liposomal transfectionTatsushi Yoshida0Kenta Yamasaki1Kenjiro Tadagaki2Department of Biochemistry and Molecular Biology, Graduate School of Medical Science, Kyoto Prefectural University of MedicineDepartment of Biochemistry and Molecular Biology, Graduate School of Medical Science, Kyoto Prefectural University of MedicineDepartment of Biochemistry and Molecular Biology, Graduate School of Medical Science, Kyoto Prefectural University of MedicineAbstract High transfection efficiency is the most important point for experiments of DNA and RNA introduction into cells. Decrease of cell viability during the transfection procedure is a crucial issue, resulting in transfection failure. However, the mechanism underlying cell growth inhibition has not been fully elucidated. Lipofection is frequently used for transfection experiments, whereases, depending on cell type, it causes a decrease in cell viability. The present study demonstrates here that a potent pan-caspase inhibitor Q-VD-OPh blocked cell death during the lipofection, indicating apoptosis was induced in lipofection. Moreover, Q-VD-OPh drastically increased transfected cells. This method provides easier and more effective transfection system of lipofection and may be useful for transfection of not only cell lines but also clinical uses such as gene therapy and nucleic acids vaccine.https://doi.org/10.1038/s41598-023-49027-y |
| spellingShingle | Tatsushi Yoshida Kenta Yamasaki Kenjiro Tadagaki Caspase inhibition improves viability and efficiency of liposomal transfection Scientific Reports |
| title | Caspase inhibition improves viability and efficiency of liposomal transfection |
| title_full | Caspase inhibition improves viability and efficiency of liposomal transfection |
| title_fullStr | Caspase inhibition improves viability and efficiency of liposomal transfection |
| title_full_unstemmed | Caspase inhibition improves viability and efficiency of liposomal transfection |
| title_short | Caspase inhibition improves viability and efficiency of liposomal transfection |
| title_sort | caspase inhibition improves viability and efficiency of liposomal transfection |
| url | https://doi.org/10.1038/s41598-023-49027-y |
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