Effects of dietary enzymatically treated Artemisia annua L. in low fish meal diet on growth, antioxidation, metabolism and intestinal health of Micropterus salmoides
The excessive substitution of fish meal (FM) by Clostridium autoethanogenum protein (CAP) could reduce the growth performance and disrupt the intestinal structural integrity of Micropterus salmoides. In order to solve this problem, an eight-week feeding trial was conducted to investigate the effects...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423003824 |
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| author | Xiaofang Dai Shujie Zhu Jingwei Ye Qiaoqing Xu Tilin Yi Chenglong Wu Benjun Wang Kai Luo Weihua Gao |
| author_facet | Xiaofang Dai Shujie Zhu Jingwei Ye Qiaoqing Xu Tilin Yi Chenglong Wu Benjun Wang Kai Luo Weihua Gao |
| author_sort | Xiaofang Dai |
| collection | DOAJ |
| description | The excessive substitution of fish meal (FM) by Clostridium autoethanogenum protein (CAP) could reduce the growth performance and disrupt the intestinal structural integrity of Micropterus salmoides. In order to solve this problem, an eight-week feeding trial was conducted to investigate the effects of dietary enzymatically treated Artemisia annua L. (EA) in low FM diet containing high-CAP on growth, antioxidation capacity, lipid and carbohydrate metabolism and intestinal microbiota of M. salmoides. Six isonitrogenous and isolipid experimental diets were produced containing 0 g/kg, 0.25 g/kg, 0.5 g/kg, 1.0 g/kg, 2.0 g/kg and 4.0 g/kg of dietary EA, which were named EA0, EA0.25, EA0.5, EA1.0, EA2.0 and EA4.0, respectively. After the feeding trial, the weight gain rate (WGR) and specific growth rate (SGR) were significantly increased in EA0.25, EA0.5 and EA 1.0 groups compared to EA0 group. The digestive enzyme activities in stomach and intestine, and the villus width and muscular layer thickness of the intestine were increased by dietary EA. Dietary EA improved the antioxidation capacity and serum lipid level of M. salmoides. The expression levels of sterol regulatory element-binding protein 1-c (srebp1-c), acetyl-coa carboxylase alpha (accα), fatty acid synthase (fan), 3-hydroxy-3-methylglutaryl-coa reductase (hmgcr) and accβ were significantly upregulated by 4.0 g/kg of dietary EA. The mRNA level of peroxisome proliferator-activated receptor alpha (pparα) and carnitine palmitoyltransferase 1 (cpt-1) were upregulated by 4.0 g/kg and 0.5 g/kg of dietary EA, respectively. Besides, the glucose content in serum was decreased by EA. The expressions of forkhead box o1 (foxo1), phosphoenolpyruvate carboxykinase (pepck), fructose bisphosphatase (fbp), glucokinase (gk) and pyruvate kinase (pk) were significantly improved by 4.0 g/kg of dietary EA, and the level of phosphofructokinase (pfk) was upregulated by 0.5 g/kg of dietary EA. The addition of 0.5 g/kg of dietary EA could optimize beneficial Cetobacterium and reduce harmful Mycoplasmataceae abundances in intestine of M. salmoides. In summary, the appropriate amount of dietary EA could be added to the high-CAP diet to improve the growth performance, antioxidation, lipid and carbohydrate metabolism, liver and intestinal health of M. salmoides. This study will provide scientific guidance for replacing FM with non-food proteins. |
| first_indexed | 2024-03-08T22:46:08Z |
| format | Article |
| id | doaj.art-4a0f920db1e0471d8a4861e1d74e5516 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:46:08Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
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| series | Aquaculture Reports |
| spelling | doaj.art-4a0f920db1e0471d8a4861e1d74e55162023-12-17T06:39:56ZengElsevierAquaculture Reports2352-51342023-12-0133101843Effects of dietary enzymatically treated Artemisia annua L. in low fish meal diet on growth, antioxidation, metabolism and intestinal health of Micropterus salmoidesXiaofang Dai0Shujie Zhu1Jingwei Ye2Qiaoqing Xu3Tilin Yi4Chenglong Wu5Benjun Wang6Kai Luo7Weihua Gao8Haiyan County Aquaculture Techniques Station of Zhejiang Province, Haiyan County Agricultural and Rural Bureau, Haiyan, ChinaHubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, ChinaHubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, ChinaHubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, ChinaHubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, ChinaCollege of Life Science, Huzhou University, 759 East 2nd Road, Huzhou 313000, ChinaHubei Wanglong Fishery Co., Ltd, ChinaHubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, China; Corresponding authors.Hubei Key Laboratory of Waterlogging Disaster and Agricultural Use of Wetland, Yangtze University, Jingzhou, China; Corresponding authors.The excessive substitution of fish meal (FM) by Clostridium autoethanogenum protein (CAP) could reduce the growth performance and disrupt the intestinal structural integrity of Micropterus salmoides. In order to solve this problem, an eight-week feeding trial was conducted to investigate the effects of dietary enzymatically treated Artemisia annua L. (EA) in low FM diet containing high-CAP on growth, antioxidation capacity, lipid and carbohydrate metabolism and intestinal microbiota of M. salmoides. Six isonitrogenous and isolipid experimental diets were produced containing 0 g/kg, 0.25 g/kg, 0.5 g/kg, 1.0 g/kg, 2.0 g/kg and 4.0 g/kg of dietary EA, which were named EA0, EA0.25, EA0.5, EA1.0, EA2.0 and EA4.0, respectively. After the feeding trial, the weight gain rate (WGR) and specific growth rate (SGR) were significantly increased in EA0.25, EA0.5 and EA 1.0 groups compared to EA0 group. The digestive enzyme activities in stomach and intestine, and the villus width and muscular layer thickness of the intestine were increased by dietary EA. Dietary EA improved the antioxidation capacity and serum lipid level of M. salmoides. The expression levels of sterol regulatory element-binding protein 1-c (srebp1-c), acetyl-coa carboxylase alpha (accα), fatty acid synthase (fan), 3-hydroxy-3-methylglutaryl-coa reductase (hmgcr) and accβ were significantly upregulated by 4.0 g/kg of dietary EA. The mRNA level of peroxisome proliferator-activated receptor alpha (pparα) and carnitine palmitoyltransferase 1 (cpt-1) were upregulated by 4.0 g/kg and 0.5 g/kg of dietary EA, respectively. Besides, the glucose content in serum was decreased by EA. The expressions of forkhead box o1 (foxo1), phosphoenolpyruvate carboxykinase (pepck), fructose bisphosphatase (fbp), glucokinase (gk) and pyruvate kinase (pk) were significantly improved by 4.0 g/kg of dietary EA, and the level of phosphofructokinase (pfk) was upregulated by 0.5 g/kg of dietary EA. The addition of 0.5 g/kg of dietary EA could optimize beneficial Cetobacterium and reduce harmful Mycoplasmataceae abundances in intestine of M. salmoides. In summary, the appropriate amount of dietary EA could be added to the high-CAP diet to improve the growth performance, antioxidation, lipid and carbohydrate metabolism, liver and intestinal health of M. salmoides. This study will provide scientific guidance for replacing FM with non-food proteins.http://www.sciencedirect.com/science/article/pii/S2352513423003824Micropterus salmoidesEnzymatically treated Artemisia annua L.GrowthRequirement |
| spellingShingle | Xiaofang Dai Shujie Zhu Jingwei Ye Qiaoqing Xu Tilin Yi Chenglong Wu Benjun Wang Kai Luo Weihua Gao Effects of dietary enzymatically treated Artemisia annua L. in low fish meal diet on growth, antioxidation, metabolism and intestinal health of Micropterus salmoides Aquaculture Reports Micropterus salmoides Enzymatically treated Artemisia annua L. Growth Requirement |
| title | Effects of dietary enzymatically treated Artemisia annua L. in low fish meal diet on growth, antioxidation, metabolism and intestinal health of Micropterus salmoides |
| title_full | Effects of dietary enzymatically treated Artemisia annua L. in low fish meal diet on growth, antioxidation, metabolism and intestinal health of Micropterus salmoides |
| title_fullStr | Effects of dietary enzymatically treated Artemisia annua L. in low fish meal diet on growth, antioxidation, metabolism and intestinal health of Micropterus salmoides |
| title_full_unstemmed | Effects of dietary enzymatically treated Artemisia annua L. in low fish meal diet on growth, antioxidation, metabolism and intestinal health of Micropterus salmoides |
| title_short | Effects of dietary enzymatically treated Artemisia annua L. in low fish meal diet on growth, antioxidation, metabolism and intestinal health of Micropterus salmoides |
| title_sort | effects of dietary enzymatically treated artemisia annua l in low fish meal diet on growth antioxidation metabolism and intestinal health of micropterus salmoides |
| topic | Micropterus salmoides Enzymatically treated Artemisia annua L. Growth Requirement |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423003824 |
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