Anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with petroleum hydrocarbons

This study included isolatation a mix cultures of sulphate reducing bacteria from soils in Shaeba regain contaminated with petroleum hydrocarbons by using API medium enriched with oxygen reducing agents and saturated with gas (90% N2 ) and ( 10% CO2 ) by using sodium lactate as sole carbon source. T...

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Main Author: Ahmad Abd Burghal Al- Asadi
Format: Article
Language:English
Published: University of Thi-Qar 2019-06-01
Series:مجلة علوم ذي قار
Online Access:https://jsci.utq.edu.iq/index.php/main/article/view/442
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author Ahmad Abd Burghal Al- Asadi
author_facet Ahmad Abd Burghal Al- Asadi
author_sort Ahmad Abd Burghal Al- Asadi
collection DOAJ
description This study included isolatation a mix cultures of sulphate reducing bacteria from soils in Shaeba regain contaminated with petroleum hydrocarbons by using API medium enriched with oxygen reducing agents and saturated with gas (90% N2 ) and ( 10% CO2 ) by using sodium lactate as sole carbon source. The result showed that the number of bacteria ranged ( 4 x 105 – 4.5 x 105 ) cells / g of soil. Also using crude oil as a sole of carbon source to study the ability of sulphate reducing bacteria on degradation of crude oil under anaerobic condition . The result showed these bacteria have high ability for degradation of crude oil. The percent rate of degradation in culture was ( 84.4 % ) after (54) days incubation and the rate concentration of total petroleum hydrocarbons ( TPH ) extraction from culture value ( 22.06 ) ?g / L , while in control was ( 190.84 ) ?g / L.
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spelling doaj.art-4a15c04349b348c081bfc76e439f16692023-11-14T08:09:25ZengUniversity of Thi-Qarمجلة علوم ذي قار1991-86902709-02562019-06-0124Anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with petroleum hydrocarbonsAhmad Abd Burghal Al- Asadi0Basrah university / College of Science/ Biology departmentThis study included isolatation a mix cultures of sulphate reducing bacteria from soils in Shaeba regain contaminated with petroleum hydrocarbons by using API medium enriched with oxygen reducing agents and saturated with gas (90% N2 ) and ( 10% CO2 ) by using sodium lactate as sole carbon source. The result showed that the number of bacteria ranged ( 4 x 105 – 4.5 x 105 ) cells / g of soil. Also using crude oil as a sole of carbon source to study the ability of sulphate reducing bacteria on degradation of crude oil under anaerobic condition . The result showed these bacteria have high ability for degradation of crude oil. The percent rate of degradation in culture was ( 84.4 % ) after (54) days incubation and the rate concentration of total petroleum hydrocarbons ( TPH ) extraction from culture value ( 22.06 ) ?g / L , while in control was ( 190.84 ) ?g / L.https://jsci.utq.edu.iq/index.php/main/article/view/442
spellingShingle Ahmad Abd Burghal Al- Asadi
Anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with petroleum hydrocarbons
مجلة علوم ذي قار
title Anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with petroleum hydrocarbons
title_full Anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with petroleum hydrocarbons
title_fullStr Anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with petroleum hydrocarbons
title_full_unstemmed Anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with petroleum hydrocarbons
title_short Anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with petroleum hydrocarbons
title_sort anaerobic degradation of crude oil by sulphate reducing bacteria isolated from soils contaminated with petroleum hydrocarbons
url https://jsci.utq.edu.iq/index.php/main/article/view/442
work_keys_str_mv AT ahmadabdburghalalasadi anaerobicdegradationofcrudeoilbysulphatereducingbacteriaisolatedfromsoilscontaminatedwithpetroleumhydrocarbons