Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers
The objective of this study was to investigate the genetic diversity and population structure of local chicken ecotypes from Burkina Faso using microsatellite markers. A total of 71 individuals representing local chicken populations from the Centre-East (18), Centre-North (17), Sahel (18) and South-...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/13/9/1523 |
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| author | Zare Yacouba Houaga Isidore Kere Michel Gnanda B. Isidore Traore Boureima Millogo Vinsoun Konkobo Maurice Bandaogo Ousseni Zongo Moussa Bougouma-Yameogo M. C. Valerie Rekaya Romdhane Nianogo A. Joseph |
| author_facet | Zare Yacouba Houaga Isidore Kere Michel Gnanda B. Isidore Traore Boureima Millogo Vinsoun Konkobo Maurice Bandaogo Ousseni Zongo Moussa Bougouma-Yameogo M. C. Valerie Rekaya Romdhane Nianogo A. Joseph |
| author_sort | Zare Yacouba |
| collection | DOAJ |
| description | The objective of this study was to investigate the genetic diversity and population structure of local chicken ecotypes from Burkina Faso using microsatellite markers. A total of 71 individuals representing local chicken populations from the Centre-East (18), Centre-North (17), Sahel (18) and South-West (18) were used to estimate genetic diversity indices, population structure and phylogenetic relationships using 20 selected polymorphic microsatellite markers. The number of alleles, mean number of alleles, mean of observed and expected heterozygosity and polymorphic information content were 127, 6.35, 0.391, 0.521, 0.539 and 0.541, respectively. The estimated overall fixation index between loci (F), among populations (F<sub>IS</sub>) and inbreeding coefficient within chicken ecotypes were 0.239, 0.267 and 0.243, respectively. Analysis of the molecular variance revealed that 77% of the total genetic diversity was attributed to within-population variation and the remaining 1% and 22% were attributed to among-regions differentiation (F<sub>ST</sub>) and among-individual differentiation (F<sub>IT</sub>), respectively. The highest pairwise genetic distance (0.026) was found between the local Konde ecotype and those from the Centre-North region while the lowest distance was observed between local chickens from the Sahel and the Centre-North regions (0.003). Neighbour-joining phylogenetic tree and principal component discriminant analyses confirmed the observed genetic distances between populations. The results show that local chickens in Burkina Faso have a rich genetic diversity with little differentiation between the studied populations. This study provides important information on measures of genetic diversity that could help in the design and implementation of future genetic improvement and conservation programs for local chickens in Burkina Faso. |
| first_indexed | 2024-03-09T23:56:20Z |
| format | Article |
| id | doaj.art-4a186b2e6747490daa08792817959f4e |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T23:56:20Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-4a186b2e6747490daa08792817959f4e2023-11-23T16:23:31ZengMDPI AGGenes2073-44252022-08-01139152310.3390/genes13091523Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite MarkersZare Yacouba0Houaga Isidore1Kere Michel2Gnanda B. Isidore3Traore Boureima4Millogo Vinsoun5Konkobo Maurice6Bandaogo Ousseni7Zongo Moussa8Bougouma-Yameogo M. C. Valerie9Rekaya Romdhane10Nianogo A. Joseph11Laboratoire de Recherche en Production et Santé Animales (LaRePSA), Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina FasoCentre for Tropical Livestock Genetics and Health (CTLGH), Roslin Institute, University of Edinburgh, Easter Bush Campus, Edinburgh EH25 9RG, UKInstitut du Développement Rural, Université Nazi BONI, Bobo-Dioulasso 01 BP 1091, Burkina FasoLaboratoire de Recherche en Production et Santé Animales (LaRePSA), Institut de l’Environnement et de Recherches Agricoles (INERA), Ouagadougou 01 BP 476, Burkina FasoLaboratoire de Physiologie Animale, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre (UFR/SVT), Université JosephKI-ZERBO, Ouagadougou 03 BP 7021, Burkina FasoInstitut du Développement Rural, Université Nazi BONI, Bobo-Dioulasso 01 BP 1091, Burkina FasoCentre International de Recherche-Développement sur l’Elevage en zone Subhumide (CIRDES), Bobo-Dioulasso 01 BP 454, Burkina FasoInstitut National de Santé Publique, Centre MURAZ, Bobo-Dioulasso 01 BP 390, Burkina FasoLaboratoire de Physiologie Animale, Unité de Formation et de Recherche en Sciences de la Vie et de la Terre (UFR/SVT), Université JosephKI-ZERBO, Ouagadougou 03 BP 7021, Burkina FasoInstitut du Développement Rural, Université Nazi BONI, Bobo-Dioulasso 01 BP 1091, Burkina FasoDepartment of Animal and Dairy Science, Department of Statistics, Institute of Bioinformatics, 106, University of Georgia, Athens, GA 30602, USAInstitut du Développement Rural, Université Nazi BONI, Bobo-Dioulasso 01 BP 1091, Burkina FasoThe objective of this study was to investigate the genetic diversity and population structure of local chicken ecotypes from Burkina Faso using microsatellite markers. A total of 71 individuals representing local chicken populations from the Centre-East (18), Centre-North (17), Sahel (18) and South-West (18) were used to estimate genetic diversity indices, population structure and phylogenetic relationships using 20 selected polymorphic microsatellite markers. The number of alleles, mean number of alleles, mean of observed and expected heterozygosity and polymorphic information content were 127, 6.35, 0.391, 0.521, 0.539 and 0.541, respectively. The estimated overall fixation index between loci (F), among populations (F<sub>IS</sub>) and inbreeding coefficient within chicken ecotypes were 0.239, 0.267 and 0.243, respectively. Analysis of the molecular variance revealed that 77% of the total genetic diversity was attributed to within-population variation and the remaining 1% and 22% were attributed to among-regions differentiation (F<sub>ST</sub>) and among-individual differentiation (F<sub>IT</sub>), respectively. The highest pairwise genetic distance (0.026) was found between the local Konde ecotype and those from the Centre-North region while the lowest distance was observed between local chickens from the Sahel and the Centre-North regions (0.003). Neighbour-joining phylogenetic tree and principal component discriminant analyses confirmed the observed genetic distances between populations. The results show that local chickens in Burkina Faso have a rich genetic diversity with little differentiation between the studied populations. This study provides important information on measures of genetic diversity that could help in the design and implementation of future genetic improvement and conservation programs for local chickens in Burkina Faso.https://www.mdpi.com/2073-4425/13/9/1523local chickenKonde chickengenetic diversitymicrosatellite markersBurkina Faso |
| spellingShingle | Zare Yacouba Houaga Isidore Kere Michel Gnanda B. Isidore Traore Boureima Millogo Vinsoun Konkobo Maurice Bandaogo Ousseni Zongo Moussa Bougouma-Yameogo M. C. Valerie Rekaya Romdhane Nianogo A. Joseph Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers Genes local chicken Konde chicken genetic diversity microsatellite markers Burkina Faso |
| title | Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers |
| title_full | Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers |
| title_fullStr | Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers |
| title_full_unstemmed | Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers |
| title_short | Genetic Diversity and Population Structure of Local Chicken Ecotypes in Burkina Faso Using Microsatellite Markers |
| title_sort | genetic diversity and population structure of local chicken ecotypes in burkina faso using microsatellite markers |
| topic | local chicken Konde chicken genetic diversity microsatellite markers Burkina Faso |
| url | https://www.mdpi.com/2073-4425/13/9/1523 |
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