Leaf extract of Strychnos ligustrina Blume inhibited Propionibacterium acnes growth in vitro
Propionibacterium acnes plays an important role in inducing skin inflammatory mediators and comedogenic acne. This study aimed to evaluate the antimicrobial effect of Strychnos ligustrina Blume leaf extract against P. acnes. This study was an in-vitro experiment with a completely randomized design (...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2023-01-01
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| Series: | E3S Web of Conferences |
| Online Access: | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/10/e3sconf_iseprolocal2023_07005.pdf |
| _version_ | 1797866493945315328 |
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| author | Hardiyanti S. Rahayuningsih S. Suherman M.P. Mardawati E. Supriadi S. Sembiring B. Harsonowati W. |
| author_facet | Hardiyanti S. Rahayuningsih S. Suherman M.P. Mardawati E. Supriadi S. Sembiring B. Harsonowati W. |
| author_sort | Hardiyanti S. |
| collection | DOAJ |
| description | Propionibacterium acnes plays an important role in inducing skin inflammatory mediators and comedogenic acne. This study aimed to evaluate the antimicrobial effect of Strychnos ligustrina Blume leaf extract against P. acnes. This study was an in-vitro experiment with a completely randomized design (CRD) testing five concentrations of ethanolic extract of S. ligustrina leaves. The control treatment was the antibiotic Kindamicin. The anti-bacterial evaluation of the extract was carried out on Mueller-Hinton agar media by using the diffusion method. The inhibition zone was assessed according to NCCLS (National Committee for Clinical Laboratory Standards). Analysis and interpretation of the results used 95% ANOVA (α: 0.05). The results of the study showed that S. ligustrina leaf extract inhibited the bacterial growth of P. acnes. The minimum concentration of the extract that inhibited the growth of P. acne was 25% with a diameter inhibition zone of 4.13 ± 0.13 mm, while the highest concentration of the undiluted extract was 7.88 ± 0.67 mm. Compared to the standard antibiotic Kindamycin (positive control), the ethanolic leaf extract of S. ligustrina was less effective having an inhibition zone of 24.81 ± 0.54 mm. The study suggests that S. ligustrina leaf extract has antimicrobial effects on P. acnes and may be further studied as an herbal remedy for acne. |
| first_indexed | 2024-04-09T23:26:05Z |
| format | Article |
| id | doaj.art-4a1b8824ef9c46a9a916421714f77ab5 |
| institution | Directory Open Access Journal |
| issn | 2267-1242 |
| language | English |
| last_indexed | 2024-04-09T23:26:05Z |
| publishDate | 2023-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | E3S Web of Conferences |
| spelling | doaj.art-4a1b8824ef9c46a9a916421714f77ab52023-03-21T10:31:46ZengEDP SciencesE3S Web of Conferences2267-12422023-01-013730700510.1051/e3sconf/202337307005e3sconf_iseprolocal2023_07005Leaf extract of Strychnos ligustrina Blume inhibited Propionibacterium acnes growth in vitroHardiyanti S.0Rahayuningsih S.1Suherman M.P.2Mardawati E.3Supriadi S.4Sembiring B.5Harsonowati W.6National Research and Innovation Agency, Research Center for Horticulture and Estate Crops, Cibinong Science CenterNational Research and Innovation Agency, Research Center for Horticulture and Estate Crops, Cibinong Science CenterPadjadjaran University, Department of Agricultural Industrial Technology, Faculty of Agricultural Industrial TechnologyPadjadjaran University, Department of Agricultural Industrial Technology, Faculty of Agricultural Industrial TechnologyNational Research and Innovation Agency, Research Center for Horticulture and Estate Crops, Cibinong Science CenterNational Research and Innovation Agency, Research Center for Agroindustry, Research Organization for Agriculture and FoodNational Research and Innovation Agency, Research Center for Horticulture and Estate Crops, Cibinong Science CenterPropionibacterium acnes plays an important role in inducing skin inflammatory mediators and comedogenic acne. This study aimed to evaluate the antimicrobial effect of Strychnos ligustrina Blume leaf extract against P. acnes. This study was an in-vitro experiment with a completely randomized design (CRD) testing five concentrations of ethanolic extract of S. ligustrina leaves. The control treatment was the antibiotic Kindamicin. The anti-bacterial evaluation of the extract was carried out on Mueller-Hinton agar media by using the diffusion method. The inhibition zone was assessed according to NCCLS (National Committee for Clinical Laboratory Standards). Analysis and interpretation of the results used 95% ANOVA (α: 0.05). The results of the study showed that S. ligustrina leaf extract inhibited the bacterial growth of P. acnes. The minimum concentration of the extract that inhibited the growth of P. acne was 25% with a diameter inhibition zone of 4.13 ± 0.13 mm, while the highest concentration of the undiluted extract was 7.88 ± 0.67 mm. Compared to the standard antibiotic Kindamycin (positive control), the ethanolic leaf extract of S. ligustrina was less effective having an inhibition zone of 24.81 ± 0.54 mm. The study suggests that S. ligustrina leaf extract has antimicrobial effects on P. acnes and may be further studied as an herbal remedy for acne.https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/10/e3sconf_iseprolocal2023_07005.pdf |
| spellingShingle | Hardiyanti S. Rahayuningsih S. Suherman M.P. Mardawati E. Supriadi S. Sembiring B. Harsonowati W. Leaf extract of Strychnos ligustrina Blume inhibited Propionibacterium acnes growth in vitro E3S Web of Conferences |
| title | Leaf extract of Strychnos ligustrina Blume inhibited Propionibacterium acnes growth in vitro |
| title_full | Leaf extract of Strychnos ligustrina Blume inhibited Propionibacterium acnes growth in vitro |
| title_fullStr | Leaf extract of Strychnos ligustrina Blume inhibited Propionibacterium acnes growth in vitro |
| title_full_unstemmed | Leaf extract of Strychnos ligustrina Blume inhibited Propionibacterium acnes growth in vitro |
| title_short | Leaf extract of Strychnos ligustrina Blume inhibited Propionibacterium acnes growth in vitro |
| title_sort | leaf extract of strychnos ligustrina blume inhibited propionibacterium acnes growth in vitro |
| url | https://www.e3s-conferences.org/articles/e3sconf/pdf/2023/10/e3sconf_iseprolocal2023_07005.pdf |
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