Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments
<i>Background Objectives:</i> The neoplastic process remains a major health problem facing humanity. Although there are currently different therapeutic options, they raise a multitude of shortcomings related to the toxic effects associated with their administration. Methotrexate (Met) an...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-01-01
|
| Series: | Medicina |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1648-9144/58/2/167 |
| _version_ | 1827654073001705472 |
|---|---|
| author | Andreea M. Kis Ioana Macasoi Corina Paul Matilda Radulescu Roxana Buzatu Claudia G. Watz Adelina Cheveresan Delia Berceanu Iulia Pinzaru Stefania Dinu Aniko Manea Marioara Poenaru Claudia Borza Cristina A. Dehelean |
| author_facet | Andreea M. Kis Ioana Macasoi Corina Paul Matilda Radulescu Roxana Buzatu Claudia G. Watz Adelina Cheveresan Delia Berceanu Iulia Pinzaru Stefania Dinu Aniko Manea Marioara Poenaru Claudia Borza Cristina A. Dehelean |
| author_sort | Andreea M. Kis |
| collection | DOAJ |
| description | <i>Background Objectives:</i> The neoplastic process remains a major health problem facing humanity. Although there are currently different therapeutic options, they raise a multitude of shortcomings related to the toxic effects associated with their administration. Methotrexate (Met) and Cetuximab (Cet) are two basic chemotherapeutics used in cancer practice, but notwithstanding despite many years of use, the mechanisms by which the multitude of side-effects occur are not yet fully understood. Thus, the present study focused on the in vitro and in ovo evaluation of the associated toxic mechanisms on keratinocytes, keys cells in the wound healing process. <i>Materials and Methods:</i> The two chemotherapeutics were tested in eight different concentrations to evaluate keratinocytes viability, the anti-migratory effect, and the influence on the expression of markers involved in the production of cell apoptosis. In addition, the potential irritating effect on the vascular plexus were highlighted by applying the in ovo method, chick chorioallantoic membrane (HET-CAM). <i>Results</i>: The results revealed that Met induced decreased cell viability as well as increased expression of pro-apoptotic genes. In the vascular plexus of the chorioallantoic membrane, Met caused vascular irritation accompanied by capillary hemorrhage and vascular stasis. <i>Conclusions</i>: Summarizing, Cet presents a safer toxicological profile, compared to Met, based on the results obtained from both in vitro (cell viability, wound healing, RT-PCR assays), and in ovo (HET-CAM assay) techniques. |
| first_indexed | 2024-03-09T21:29:24Z |
| format | Article |
| id | doaj.art-4a1b918cbca0486fa439d64c34d52765 |
| institution | Directory Open Access Journal |
| issn | 1010-660X 1648-9144 |
| language | English |
| last_indexed | 2024-03-09T21:29:24Z |
| publishDate | 2022-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Medicina |
| spelling | doaj.art-4a1b918cbca0486fa439d64c34d527652023-11-23T20:59:03ZengMDPI AGMedicina1010-660X1648-91442022-01-0158216710.3390/medicina58020167Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo AssessmentsAndreea M. Kis0Ioana Macasoi1Corina Paul2Matilda Radulescu3Roxana Buzatu4Claudia G. Watz5Adelina Cheveresan6Delia Berceanu7Iulia Pinzaru8Stefania Dinu9Aniko Manea10Marioara Poenaru11Claudia Borza12Cristina A. Dehelean13Department of ENT, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timișoara, RomaniaDepartament of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Pediatrics, “Victor Babeş” University of Medicine and Pharmacy, Eftimie Murgu Square No. 2, 300041 Timișoara, RomaniaDepartment of Microbiology, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, RomaniaDepartment of Dental Aesthetics, Faculty of Dental Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, RomaniaResearch Center for Pharmaco-Toxicological Evaluations, Faculty of Pharmacy, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, RomaniaDepartment of Pharmacology, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, RomaniaDepartment of Microbiology, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, RomaniaDepartament of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, RomaniaDepartment of Pedodontics, Faculty of Dental Medicine, “Victor Babeş” University of Medicine and Pharmacy Timisoara, 9 No., Revolutiei Bv., 300041 Timişoara, RomaniaDepartment of Neonatology, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, RomaniaDepartment of ENT, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timișoara, RomaniaDepartment of Pathophysiology, Faculty of Medicine, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timisoara, RomaniaDepartament of Toxicology and Drug Industry, Faculty of Pharmacy, “Victor Babeş” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square No. 2, 300041 Timişoara, Romania<i>Background Objectives:</i> The neoplastic process remains a major health problem facing humanity. Although there are currently different therapeutic options, they raise a multitude of shortcomings related to the toxic effects associated with their administration. Methotrexate (Met) and Cetuximab (Cet) are two basic chemotherapeutics used in cancer practice, but notwithstanding despite many years of use, the mechanisms by which the multitude of side-effects occur are not yet fully understood. Thus, the present study focused on the in vitro and in ovo evaluation of the associated toxic mechanisms on keratinocytes, keys cells in the wound healing process. <i>Materials and Methods:</i> The two chemotherapeutics were tested in eight different concentrations to evaluate keratinocytes viability, the anti-migratory effect, and the influence on the expression of markers involved in the production of cell apoptosis. In addition, the potential irritating effect on the vascular plexus were highlighted by applying the in ovo method, chick chorioallantoic membrane (HET-CAM). <i>Results</i>: The results revealed that Met induced decreased cell viability as well as increased expression of pro-apoptotic genes. In the vascular plexus of the chorioallantoic membrane, Met caused vascular irritation accompanied by capillary hemorrhage and vascular stasis. <i>Conclusions</i>: Summarizing, Cet presents a safer toxicological profile, compared to Met, based on the results obtained from both in vitro (cell viability, wound healing, RT-PCR assays), and in ovo (HET-CAM assay) techniques.https://www.mdpi.com/1648-9144/58/2/167methotrexatecetuximabHaCaTcell viabilityHET-CAM assayRT-PCR |
| spellingShingle | Andreea M. Kis Ioana Macasoi Corina Paul Matilda Radulescu Roxana Buzatu Claudia G. Watz Adelina Cheveresan Delia Berceanu Iulia Pinzaru Stefania Dinu Aniko Manea Marioara Poenaru Claudia Borza Cristina A. Dehelean Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments Medicina methotrexate cetuximab HaCaT cell viability HET-CAM assay RT-PCR |
| title | Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments |
| title_full | Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments |
| title_fullStr | Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments |
| title_full_unstemmed | Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments |
| title_short | Methotrexate and Cetuximab—Biological Impact on Non-Tumorigenic Models: In Vitro and In Ovo Assessments |
| title_sort | methotrexate and cetuximab biological impact on non tumorigenic models in vitro and in ovo assessments |
| topic | methotrexate cetuximab HaCaT cell viability HET-CAM assay RT-PCR |
| url | https://www.mdpi.com/1648-9144/58/2/167 |
| work_keys_str_mv | AT andreeamkis methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT ioanamacasoi methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT corinapaul methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT matildaradulescu methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT roxanabuzatu methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT claudiagwatz methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT adelinacheveresan methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT deliaberceanu methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT iuliapinzaru methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT stefaniadinu methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT anikomanea methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT marioarapoenaru methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT claudiaborza methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments AT cristinaadehelean methotrexateandcetuximabbiologicalimpactonnontumorigenicmodelsinvitroandinovoassessments |