Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations
İpek Özcan, Erkan Azizoğlu, Taner Şenyiğit, Mine Özyazıcı, Özgen ÖzerEge University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Bornova, Izmir, TurkeyAbstract: The objective of this study was to prepare a suitable formulation for...
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| Format: | Article |
| Language: | English |
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Dove Medical Press
2013-01-01
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| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/enhanced-dermal-delivery-of-diflucortolone-valerate-using-lecithinchit-a12082 |
| _version_ | 1818570755183476736 |
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| author | Özcan İ Azizoğlu E Şenyiğit T Özyazıcı M Özer Ö |
| author_facet | Özcan İ Azizoğlu E Şenyiğit T Özyazıcı M Özer Ö |
| author_sort | Özcan İ |
| collection | DOAJ |
| description | İpek Özcan, Erkan Azizoğlu, Taner Şenyiğit, Mine Özyazıcı, Özgen ÖzerEge University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Bornova, Izmir, TurkeyAbstract: The objective of this study was to prepare a suitable formulation for dermal delivery of diflucortolone valerate (DFV) that would maintain the localization in skin layers without any penetration and to optimize efficiency of DFV. Drug-loaded lecithin/chitosan nanoparticles with high entrapment efficiency (86.8%), were successfully prepared by ionic interaction technique. Sustained release of DFV was achieved without any initial burst release. Nanoparticles were also incorporated into chitosan gel at different ratios for preparing a more suitable formulation for topical drug delivery with adequate viscosity. In ex-vivo permeation studies, nanoparticles increased the accumulation of DFV especially in the stratum corneum + epidermis of rat skin without any significant permeation. Retention of DFV from nanoparticle in chitosan gel formulation (0.01%) was twofold higher than commercial cream, although it contained ten times less DFV. Nanoparticles in gel formulations produced significantly higher edema inhibition in rats compared with commercial cream in in-vivo studies. Skin blanching assay using a chromameter showed vasoconstriction similar to that of the commercial product. There were no barrier function changes upon application of nanoparticles. In-vitro and in-vivo results demonstrated that lecithin/chitosan nanoparticles in chitosan gel may be a promising carrier for dermal delivery of DFV in various skin disorders.Keywords: skin permeation, anti-inflammatory activity, skin blanching, TEWL |
| first_indexed | 2024-12-14T13:46:05Z |
| format | Article |
| id | doaj.art-4a1c4cdf070a489ea4ec5b4a07660cda |
| institution | Directory Open Access Journal |
| issn | 1176-9114 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T13:46:05Z |
| publishDate | 2013-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-4a1c4cdf070a489ea4ec5b4a07660cda2022-12-21T22:59:19ZengDove Medical PressInternational Journal of Nanomedicine1176-91141178-20132013-01-012013default461475Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluationsÖzcan İAzizoğlu EŞenyiğit TÖzyazıcı MÖzer Öİpek Özcan, Erkan Azizoğlu, Taner Şenyiğit, Mine Özyazıcı, Özgen ÖzerEge University, Faculty of Pharmacy, Department of Pharmaceutical Technology, Bornova, Izmir, TurkeyAbstract: The objective of this study was to prepare a suitable formulation for dermal delivery of diflucortolone valerate (DFV) that would maintain the localization in skin layers without any penetration and to optimize efficiency of DFV. Drug-loaded lecithin/chitosan nanoparticles with high entrapment efficiency (86.8%), were successfully prepared by ionic interaction technique. Sustained release of DFV was achieved without any initial burst release. Nanoparticles were also incorporated into chitosan gel at different ratios for preparing a more suitable formulation for topical drug delivery with adequate viscosity. In ex-vivo permeation studies, nanoparticles increased the accumulation of DFV especially in the stratum corneum + epidermis of rat skin without any significant permeation. Retention of DFV from nanoparticle in chitosan gel formulation (0.01%) was twofold higher than commercial cream, although it contained ten times less DFV. Nanoparticles in gel formulations produced significantly higher edema inhibition in rats compared with commercial cream in in-vivo studies. Skin blanching assay using a chromameter showed vasoconstriction similar to that of the commercial product. There were no barrier function changes upon application of nanoparticles. In-vitro and in-vivo results demonstrated that lecithin/chitosan nanoparticles in chitosan gel may be a promising carrier for dermal delivery of DFV in various skin disorders.Keywords: skin permeation, anti-inflammatory activity, skin blanching, TEWLhttp://www.dovepress.com/enhanced-dermal-delivery-of-diflucortolone-valerate-using-lecithinchit-a12082 |
| spellingShingle | Özcan İ Azizoğlu E Şenyiğit T Özyazıcı M Özer Ö Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations International Journal of Nanomedicine |
| title | Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations |
| title_full | Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations |
| title_fullStr | Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations |
| title_full_unstemmed | Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations |
| title_short | Enhanced dermal delivery of diflucortolone valerate using lecithin/chitosan nanoparticles: in-vitro and in-vivo evaluations |
| title_sort | enhanced dermal delivery of diflucortolone valerate using lecithin chitosan nanoparticles in vitro and in vivo evaluations |
| url | http://www.dovepress.com/enhanced-dermal-delivery-of-diflucortolone-valerate-using-lecithinchit-a12082 |
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