Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin
Mesoporous Bioactive Glasses (MBGs) are a family of bioceramics widely investigated for their putative clinical use as scaffolds for bone regeneration. Their outstanding textural properties allow for high bioactivity when compared with other bioactive materials. Moreover, their great pore volumes al...
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| Format: | Article |
| Language: | English |
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MDPI AG
2018-08-01
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| Series: | Nanomaterials |
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| Online Access: | http://www.mdpi.com/2079-4991/8/8/592 |
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| author | Rebeca Pérez Sandra Sanchez-Salcedo Daniel Lozano Clara Heras Pedro Esbrit María Vallet-Regí Antonio J. Salinas |
| author_facet | Rebeca Pérez Sandra Sanchez-Salcedo Daniel Lozano Clara Heras Pedro Esbrit María Vallet-Regí Antonio J. Salinas |
| author_sort | Rebeca Pérez |
| collection | DOAJ |
| description | Mesoporous Bioactive Glasses (MBGs) are a family of bioceramics widely investigated for their putative clinical use as scaffolds for bone regeneration. Their outstanding textural properties allow for high bioactivity when compared with other bioactive materials. Moreover, their great pore volumes allow these glasses to be loaded with a wide range of biomolecules to stimulate new bone formation. In this study, an MBG with a composition, in mol%, of 80% SiO2–15% CaO–5% P2O5 (Blank, BL) was compared with two analogous glasses containing 4% and 5% of ZnO (4ZN and 5ZN) before and after impregnation with osteostatin, a C-terminal peptide from a parathyroid hormone-related protein (PTHrP107-111). Zn2+ ions were included in the glass for their bone growth stimulator properties, whereas osteostatin was added for its osteogenic properties. Glasses were characterized, and their cytocompatibility investigated, in pre-osteoblastic MC3T3-E1 cell cultures. The simultaneous additions of osteostatin and Zn2+ ions provoked enhanced MC3T3-E1 cell viability and a higher differentiation capacity, compared with either raw BL or MBGs supplemented only with osteostatin or Zn2+. These in vitro results show that osteostatin enhances the osteogenic effect of Zn2+-enriched glasses, suggesting the potential of this combined approach in bone tissue engineering applications. |
| first_indexed | 2024-12-11T23:25:35Z |
| format | Article |
| id | doaj.art-4a1de960c414486781e797d0e30781bd |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-12-11T23:25:35Z |
| publishDate | 2018-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-4a1de960c414486781e797d0e30781bd2022-12-22T00:46:12ZengMDPI AGNanomaterials2079-49912018-08-018859210.3390/nano8080592nano8080592Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with OsteostatinRebeca Pérez0Sandra Sanchez-Salcedo1Daniel Lozano2Clara Heras3Pedro Esbrit4María Vallet-Regí5Antonio J. Salinas6Departamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, UCM, Instituto de Investigación Hospital 12 de Octubre, imas12, 28040 Madrid, SpainDepartamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, UCM, Instituto de Investigación Hospital 12 de Octubre, imas12, 28040 Madrid, SpainDepartamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, UCM, Instituto de Investigación Hospital 12 de Octubre, imas12, 28040 Madrid, SpainDepartamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, UCM, Instituto de Investigación Hospital 12 de Octubre, imas12, 28040 Madrid, SpainDepartamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, UCM, Instituto de Investigación Hospital 12 de Octubre, imas12, 28040 Madrid, SpainDepartamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, UCM, Instituto de Investigación Hospital 12 de Octubre, imas12, 28040 Madrid, SpainDepartamento de Química en Ciencias Farmacéuticas, Facultad de Farmacia, Universidad Complutense de Madrid, UCM, Instituto de Investigación Hospital 12 de Octubre, imas12, 28040 Madrid, SpainMesoporous Bioactive Glasses (MBGs) are a family of bioceramics widely investigated for their putative clinical use as scaffolds for bone regeneration. Their outstanding textural properties allow for high bioactivity when compared with other bioactive materials. Moreover, their great pore volumes allow these glasses to be loaded with a wide range of biomolecules to stimulate new bone formation. In this study, an MBG with a composition, in mol%, of 80% SiO2–15% CaO–5% P2O5 (Blank, BL) was compared with two analogous glasses containing 4% and 5% of ZnO (4ZN and 5ZN) before and after impregnation with osteostatin, a C-terminal peptide from a parathyroid hormone-related protein (PTHrP107-111). Zn2+ ions were included in the glass for their bone growth stimulator properties, whereas osteostatin was added for its osteogenic properties. Glasses were characterized, and their cytocompatibility investigated, in pre-osteoblastic MC3T3-E1 cell cultures. The simultaneous additions of osteostatin and Zn2+ ions provoked enhanced MC3T3-E1 cell viability and a higher differentiation capacity, compared with either raw BL or MBGs supplemented only with osteostatin or Zn2+. These in vitro results show that osteostatin enhances the osteogenic effect of Zn2+-enriched glasses, suggesting the potential of this combined approach in bone tissue engineering applications.http://www.mdpi.com/2079-4991/8/8/592mesoporous glassesZnO-additionsosteostatin loadingosteosteoblast cell culturesosteogenic effect |
| spellingShingle | Rebeca Pérez Sandra Sanchez-Salcedo Daniel Lozano Clara Heras Pedro Esbrit María Vallet-Regí Antonio J. Salinas Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin Nanomaterials mesoporous glasses ZnO-additions osteostatin loading osteosteoblast cell cultures osteogenic effect |
| title | Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin |
| title_full | Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin |
| title_fullStr | Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin |
| title_full_unstemmed | Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin |
| title_short | Osteogenic Effect of ZnO-Mesoporous Glasses Loaded with Osteostatin |
| title_sort | osteogenic effect of zno mesoporous glasses loaded with osteostatin |
| topic | mesoporous glasses ZnO-additions osteostatin loading osteosteoblast cell cultures osteogenic effect |
| url | http://www.mdpi.com/2079-4991/8/8/592 |
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