Effects of Virgin Olive Oil on Blood Pressure and Renal Aminopeptidase Activities in Male Wistar Rats
High saturated fat diets have been associated with the development of obesity and hypertension, along with other pathologies related to the metabolic syndrome. In contrast, the Mediterranean diet, characterized by its high content of monounsaturated fatty acids, has been proposed as a dietary factor...
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MDPI AG
2021-05-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/10/5388 |
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| author | Germán Domínguez-Vías Ana Belén Segarra Manuel Ramírez-Sánchez Isabel Prieto |
| author_facet | Germán Domínguez-Vías Ana Belén Segarra Manuel Ramírez-Sánchez Isabel Prieto |
| author_sort | Germán Domínguez-Vías |
| collection | DOAJ |
| description | High saturated fat diets have been associated with the development of obesity and hypertension, along with other pathologies related to the metabolic syndrome. In contrast, the Mediterranean diet, characterized by its high content of monounsaturated fatty acids, has been proposed as a dietary factor capable of positively regulating cardiovascular function. These effects have been linked to changes in the local renal renin angiotensin system (RAS) and the activity of the sympathetic nervous system. The main goal of this study was to analyze the role of two dietary fat sources on aminopeptidases activities involved in local kidney RAS. Male Wistar rats (six months old) were fed during 24 weeks with three different diets: the standard diet (S), the standard diet supplemented with virgin olive oil (20%) (VOO), or the standard diet enriched with butter (20%) plus cholesterol (0.1%) (Bch). Kidney samples were separated in medulla and cortex for aminopeptidase activities (AP) assay. Urine samples were collected for routine analysis by chemical tests. Aminopeptidase activities were determined by fluorometric methods in soluble (sol) and membrane-bound (mb) fractions of renal tissue, using arylamide derivatives as substrates. After the experimental period, the systolic blood pressure (SBP) values were similar in standard and VOO animals, and significantly lower than in the Bch group. At the same time, a significant increase in GluAP and IRAP activities were found in renal medulla of Bch animals. However, in VOO group the increase of GluAP activity in renal medulla was lower, while AspAP activity decreased in the renal cortex. Furthermore, the VOO diet also affected other aminopeptidase activities, such as TyrAP and pGluAP, related to the regulation of the sympathetic nervous system and the metabolic rate. These results support the beneficial effect of VOO in the regulation of SBP through changes in local AP activities of the kidney. |
| first_indexed | 2024-03-10T11:13:07Z |
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| language | English |
| last_indexed | 2024-03-10T11:13:07Z |
| publishDate | 2021-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-4a1e2d96e98340d49e5e2501922d26722023-11-21T20:36:28ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-05-012210538810.3390/ijms22105388Effects of Virgin Olive Oil on Blood Pressure and Renal Aminopeptidase Activities in Male Wistar RatsGermán Domínguez-Vías0Ana Belén Segarra1Manuel Ramírez-Sánchez2Isabel Prieto3Unit of Physiology, Department of Health Sciences, University of Jaén, Las Lagunillas, 23071 Jaén, SpainUnit of Physiology, Department of Health Sciences, University of Jaén, Las Lagunillas, 23071 Jaén, SpainUnit of Physiology, Department of Health Sciences, University of Jaén, Las Lagunillas, 23071 Jaén, SpainUnit of Physiology, Department of Health Sciences, University of Jaén, Las Lagunillas, 23071 Jaén, SpainHigh saturated fat diets have been associated with the development of obesity and hypertension, along with other pathologies related to the metabolic syndrome. In contrast, the Mediterranean diet, characterized by its high content of monounsaturated fatty acids, has been proposed as a dietary factor capable of positively regulating cardiovascular function. These effects have been linked to changes in the local renal renin angiotensin system (RAS) and the activity of the sympathetic nervous system. The main goal of this study was to analyze the role of two dietary fat sources on aminopeptidases activities involved in local kidney RAS. Male Wistar rats (six months old) were fed during 24 weeks with three different diets: the standard diet (S), the standard diet supplemented with virgin olive oil (20%) (VOO), or the standard diet enriched with butter (20%) plus cholesterol (0.1%) (Bch). Kidney samples were separated in medulla and cortex for aminopeptidase activities (AP) assay. Urine samples were collected for routine analysis by chemical tests. Aminopeptidase activities were determined by fluorometric methods in soluble (sol) and membrane-bound (mb) fractions of renal tissue, using arylamide derivatives as substrates. After the experimental period, the systolic blood pressure (SBP) values were similar in standard and VOO animals, and significantly lower than in the Bch group. At the same time, a significant increase in GluAP and IRAP activities were found in renal medulla of Bch animals. However, in VOO group the increase of GluAP activity in renal medulla was lower, while AspAP activity decreased in the renal cortex. Furthermore, the VOO diet also affected other aminopeptidase activities, such as TyrAP and pGluAP, related to the regulation of the sympathetic nervous system and the metabolic rate. These results support the beneficial effect of VOO in the regulation of SBP through changes in local AP activities of the kidney.https://www.mdpi.com/1422-0067/22/10/5388renal–aminopeptidase activitiessystolic blood pressurehigh-fat dietvirgin olive oil |
| spellingShingle | Germán Domínguez-Vías Ana Belén Segarra Manuel Ramírez-Sánchez Isabel Prieto Effects of Virgin Olive Oil on Blood Pressure and Renal Aminopeptidase Activities in Male Wistar Rats International Journal of Molecular Sciences renal–aminopeptidase activities systolic blood pressure high-fat diet virgin olive oil |
| title | Effects of Virgin Olive Oil on Blood Pressure and Renal Aminopeptidase Activities in Male Wistar Rats |
| title_full | Effects of Virgin Olive Oil on Blood Pressure and Renal Aminopeptidase Activities in Male Wistar Rats |
| title_fullStr | Effects of Virgin Olive Oil on Blood Pressure and Renal Aminopeptidase Activities in Male Wistar Rats |
| title_full_unstemmed | Effects of Virgin Olive Oil on Blood Pressure and Renal Aminopeptidase Activities in Male Wistar Rats |
| title_short | Effects of Virgin Olive Oil on Blood Pressure and Renal Aminopeptidase Activities in Male Wistar Rats |
| title_sort | effects of virgin olive oil on blood pressure and renal aminopeptidase activities in male wistar rats |
| topic | renal–aminopeptidase activities systolic blood pressure high-fat diet virgin olive oil |
| url | https://www.mdpi.com/1422-0067/22/10/5388 |
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