Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures
BACKGROUND: Candida spp and Malassezia spp cause superficial infections that may be resistant to conventional treatments. Violet light-emitting diodes (LEDs) therapy is a therapeutic alternative. PURPOSE: To describe the protocol for evaluating the antifungal effect of violet LEDs 410 nm ± 10 nm on...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Escola Bahiana de Medicina e Saúde Pública
2022-12-01
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| Series: | Journal of Evidence-Based Healthcare |
| Subjects: | |
| Online Access: | https://www5.bahiana.edu.br/index.php/evidence/article/view/4736 |
| _version_ | 1797856542469390336 |
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| author | Rachel Trinchão Schneiberg Kalid Ribeiro Élissa da Silva Santos Rita Elizabeth Moreira Mascarenhas Marília Wellichan Mancini Luciana Almeida-Lopes Tânia Fraga Barros Carlos Gustavo Regis Jacqueline de Jesus Silva Diogo Rodrigo de Magalhães Moreira Daniel Oliveira Dantas Beatriz Trinchão Andrade Jéssica Mirella de Souza Gomes Cristiane Maria Carvalho Costa Dias Maria Fernanda Rios Grassi |
| author_facet | Rachel Trinchão Schneiberg Kalid Ribeiro Élissa da Silva Santos Rita Elizabeth Moreira Mascarenhas Marília Wellichan Mancini Luciana Almeida-Lopes Tânia Fraga Barros Carlos Gustavo Regis Jacqueline de Jesus Silva Diogo Rodrigo de Magalhães Moreira Daniel Oliveira Dantas Beatriz Trinchão Andrade Jéssica Mirella de Souza Gomes Cristiane Maria Carvalho Costa Dias Maria Fernanda Rios Grassi |
| author_sort | Rachel Trinchão Schneiberg Kalid Ribeiro |
| collection | DOAJ |
| description |
BACKGROUND: Candida spp and Malassezia spp cause superficial infections that may be resistant to conventional treatments. Violet light-emitting diodes (LEDs) therapy is a therapeutic alternative. PURPOSE: To describe the protocol for evaluating the antifungal effect of violet LEDs 410 nm ± 10 nm on Candida spp and Malassezia spp in vitro. PROTOCOL: LEDs 410 nm ± 10 nm are applied to a fungal suspension at fluences of 61.13 J/cm2, 91.70 J/cm2, and 183.39 J/cm2. The isolates are cultured for 48 to 72 hours. Colony forming units (CFUs) are quantified by visual counting and percent culture plate occupancy by digital analysis. Morphology is assessed by light microscopy and Gram staining, and yeast metabolism/function by transmission electron microscopy, assessment of reactive oxygen species, and DNA fragmentation. DATA ANALYSIS: the percentage of LEDs inhibition is calculated considering the growth of the negative control condition and the percentage of plate occupancy by yeasts by dividing the number of pixels classified as colonies by the total number of pixels on the plate. The morphological and functional aspects are described for the intervention and negative control. The ANOVA test is used to compare the mean percentages of growth inhibition and plate occupancy between the three fluences of LEDs 410 nm ± 10 nm and the negative control. ESTIMATED RESULTS: We intend to determine the antifungal effect of the different fluences of LEDs 410 nm ± 10 nm on Candida spp and Malassezia spp. The evaluation of other fungal species by this protocol should be investigated.
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| first_indexed | 2024-04-09T20:42:11Z |
| format | Article |
| id | doaj.art-4a1ef426b7c94b1cba022f1c538054b8 |
| institution | Directory Open Access Journal |
| issn | 2675-021X |
| language | English |
| last_indexed | 2024-04-09T20:42:11Z |
| publishDate | 2022-12-01 |
| publisher | Escola Bahiana de Medicina e Saúde Pública |
| record_format | Article |
| series | Journal of Evidence-Based Healthcare |
| spelling | doaj.art-4a1ef426b7c94b1cba022f1c538054b82023-03-29T22:06:12ZengEscola Bahiana de Medicina e Saúde PúblicaJournal of Evidence-Based Healthcare2675-021X2022-12-01410.17267/2675-021Xevidence.2022.e4736Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures Rachel Trinchão Schneiberg Kalid Ribeiro0Élissa da Silva Santos1Rita Elizabeth Moreira Mascarenhas2Marília Wellichan Mancini3Luciana Almeida-Lopes4Tânia Fraga Barros5Carlos Gustavo Regis6Jacqueline de Jesus Silva7Diogo Rodrigo de Magalhães Moreira8Daniel Oliveira Dantas9Beatriz Trinchão Andrade10Jéssica Mirella de Souza Gomes11Cristiane Maria Carvalho Costa Dias12Maria Fernanda Rios Grassi13Bahia School of Medicine and Public Health. Salvador, BahiaBahiana School of Medicine and Public Health. Salvador, BahiaBahiana School of Medicine and Public Health. Salvador, BahiaInstituto de Pesquisas e Ensino na Área da Saúde - NUPEN. (São Carlos). São Paulo, Brasil.Instituto de Pesquisas e Ensino na Área da Saúde - NUPEN. (São Carlos). São Paulo, Brasil.Universidade Federal da Bahia (Salvador). Bahia, Brasil.Fundação Oswaldo Cruz. (Salvador). Bahia, Brasil.Fundação Oswaldo Cruz (Salvador). Bahia, Brasil.Fundação Oswaldo Cruz (Salvador). Bahia, Brasil.Universidade Federal de Sergipe (Aracaju). Sergipe, Brasil.Universidade Federal de Sergipe (Aracaju). Sergipe, Brasil.Escola Bahiana de Medicina e Saúde Pública (Salvador). Bahia, Brasil.Escola Bahiana de Medicina e Saúde Pública (Salvador). Bahia, Brasil.Fundação Oswaldo Cruz (Salvador). Bahia, Brasil. BACKGROUND: Candida spp and Malassezia spp cause superficial infections that may be resistant to conventional treatments. Violet light-emitting diodes (LEDs) therapy is a therapeutic alternative. PURPOSE: To describe the protocol for evaluating the antifungal effect of violet LEDs 410 nm ± 10 nm on Candida spp and Malassezia spp in vitro. PROTOCOL: LEDs 410 nm ± 10 nm are applied to a fungal suspension at fluences of 61.13 J/cm2, 91.70 J/cm2, and 183.39 J/cm2. The isolates are cultured for 48 to 72 hours. Colony forming units (CFUs) are quantified by visual counting and percent culture plate occupancy by digital analysis. Morphology is assessed by light microscopy and Gram staining, and yeast metabolism/function by transmission electron microscopy, assessment of reactive oxygen species, and DNA fragmentation. DATA ANALYSIS: the percentage of LEDs inhibition is calculated considering the growth of the negative control condition and the percentage of plate occupancy by yeasts by dividing the number of pixels classified as colonies by the total number of pixels on the plate. The morphological and functional aspects are described for the intervention and negative control. The ANOVA test is used to compare the mean percentages of growth inhibition and plate occupancy between the three fluences of LEDs 410 nm ± 10 nm and the negative control. ESTIMATED RESULTS: We intend to determine the antifungal effect of the different fluences of LEDs 410 nm ± 10 nm on Candida spp and Malassezia spp. The evaluation of other fungal species by this protocol should be investigated. https://www5.bahiana.edu.br/index.php/evidence/article/view/4736PhototherapyAntifungal agentsCandidaMalassezia |
| spellingShingle | Rachel Trinchão Schneiberg Kalid Ribeiro Élissa da Silva Santos Rita Elizabeth Moreira Mascarenhas Marília Wellichan Mancini Luciana Almeida-Lopes Tânia Fraga Barros Carlos Gustavo Regis Jacqueline de Jesus Silva Diogo Rodrigo de Magalhães Moreira Daniel Oliveira Dantas Beatriz Trinchão Andrade Jéssica Mirella de Souza Gomes Cristiane Maria Carvalho Costa Dias Maria Fernanda Rios Grassi Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures Journal of Evidence-Based Healthcare Phototherapy Antifungal agents Candida Malassezia |
| title | Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures |
| title_full | Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures |
| title_fullStr | Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures |
| title_full_unstemmed | Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures |
| title_short | Protocol for evaluating the in vitro effect of violet light-emitting diodes (LEDs) 410 nm ± 10 nm on yeast cultures |
| title_sort | protocol for evaluating the in vitro effect of violet light emitting diodes leds 410 nm 10 nm on yeast cultures |
| topic | Phototherapy Antifungal agents Candida Malassezia |
| url | https://www5.bahiana.edu.br/index.php/evidence/article/view/4736 |
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