Intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in RCS rats
Abstract Background Human umbilical cord mesenchymal stem cells (UCMSCs) transplantation is a promising therapy for the treatment of retinitis pigmentosa (RP). However, intravenously infused cells may be blocked in the lung, increasing the risk of vascular obstruction, which needs to be optimized to...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-02-01
|
| Series: | BMC Ophthalmology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12886-021-02171-3 |
| _version_ | 1818973779338985472 |
|---|---|
| author | Qingling Liang Qiyou Li Bangqi Ren Zheng Qin Yin |
| author_facet | Qingling Liang Qiyou Li Bangqi Ren Zheng Qin Yin |
| author_sort | Qingling Liang |
| collection | DOAJ |
| description | Abstract Background Human umbilical cord mesenchymal stem cells (UCMSCs) transplantation is a promising therapy for the treatment of retinitis pigmentosa (RP). However, intravenously infused cells may be blocked in the lung, increasing the risk of vascular obstruction, which needs to be optimized to further improve safety and efficacy. Methods We derived small UCMSCs (S-UCMSCs) from filtering UCMSCs with a 10-μm filter, and compared with UCMSCs by flow cytometry, directional differentiation culture and transcriptome sequencing. Then the S-UCMSCs and UCMSCs were intravenously infused in the Royal College Surgeons (RCS) rats to evaluate the safety and the efficacy. Results The diameter of S-UCMSCs ranged from 5.568 to 17.231 μm, with an average diameter of 8.636 ± 2.256 μm, which was significantly smaller than that of UCMSCs. Flow cytometry, immunofluorescence and transcriptome sequencing demonstrated that the S-UCMSCs and UCMSCs were the same kind of MSCs, and the S-UCMSCs were more proliferative. After the S-UCMSCs and UCMSCs were intravenously infused into the Royal College of Surgeons (RCS) rats at a dose of 1 × 106 cells/rat, the S-UCMSCs blocked in the lungs were significantly fewer and disappeared more quickly than UCMSCs. The b wave of the flash electroretinogram was improved at 7 d, and the retinal outer nuclear layer thickness was thicker at 7 d and 14 d. The expression level of inflammation was inhibited, and the expression level of neurotrophic factors was upregulated in the retina and serum after transplantation. Conclusions S-UCMSCs intravenous infusion was safer than UCMSCs and could delay retinal degeneration and protect visual function in RCS rats, which may be a preferable therapeutic approach for RP. |
| first_indexed | 2024-12-20T15:29:35Z |
| format | Article |
| id | doaj.art-4a2166d69a3340fca0d6a434f746211c |
| institution | Directory Open Access Journal |
| issn | 1471-2415 |
| language | English |
| last_indexed | 2024-12-20T15:29:35Z |
| publishDate | 2022-02-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Ophthalmology |
| spelling | doaj.art-4a2166d69a3340fca0d6a434f746211c2022-12-21T19:35:39ZengBMCBMC Ophthalmology1471-24152022-02-0122111110.1186/s12886-021-02171-3Intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in RCS ratsQingling Liang0Qiyou Li1Bangqi Ren2Zheng Qin Yin3Southwest Hospital/Southwest Eye Hospital, Third Military Medical University (Amy Medical University)Southwest Hospital/Southwest Eye Hospital, Third Military Medical University (Amy Medical University)Southwest Hospital/Southwest Eye Hospital, Third Military Medical University (Amy Medical University)Southwest Hospital/Southwest Eye Hospital, Third Military Medical University (Amy Medical University)Abstract Background Human umbilical cord mesenchymal stem cells (UCMSCs) transplantation is a promising therapy for the treatment of retinitis pigmentosa (RP). However, intravenously infused cells may be blocked in the lung, increasing the risk of vascular obstruction, which needs to be optimized to further improve safety and efficacy. Methods We derived small UCMSCs (S-UCMSCs) from filtering UCMSCs with a 10-μm filter, and compared with UCMSCs by flow cytometry, directional differentiation culture and transcriptome sequencing. Then the S-UCMSCs and UCMSCs were intravenously infused in the Royal College Surgeons (RCS) rats to evaluate the safety and the efficacy. Results The diameter of S-UCMSCs ranged from 5.568 to 17.231 μm, with an average diameter of 8.636 ± 2.256 μm, which was significantly smaller than that of UCMSCs. Flow cytometry, immunofluorescence and transcriptome sequencing demonstrated that the S-UCMSCs and UCMSCs were the same kind of MSCs, and the S-UCMSCs were more proliferative. After the S-UCMSCs and UCMSCs were intravenously infused into the Royal College of Surgeons (RCS) rats at a dose of 1 × 106 cells/rat, the S-UCMSCs blocked in the lungs were significantly fewer and disappeared more quickly than UCMSCs. The b wave of the flash electroretinogram was improved at 7 d, and the retinal outer nuclear layer thickness was thicker at 7 d and 14 d. The expression level of inflammation was inhibited, and the expression level of neurotrophic factors was upregulated in the retina and serum after transplantation. Conclusions S-UCMSCs intravenous infusion was safer than UCMSCs and could delay retinal degeneration and protect visual function in RCS rats, which may be a preferable therapeutic approach for RP.https://doi.org/10.1186/s12886-021-02171-3Umbilical cord mesenchymal stem cellsIntravenous infusionSafetyEfficacyRetinitis pigmentosa |
| spellingShingle | Qingling Liang Qiyou Li Bangqi Ren Zheng Qin Yin Intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in RCS rats BMC Ophthalmology Umbilical cord mesenchymal stem cells Intravenous infusion Safety Efficacy Retinitis pigmentosa |
| title | Intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in RCS rats |
| title_full | Intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in RCS rats |
| title_fullStr | Intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in RCS rats |
| title_full_unstemmed | Intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in RCS rats |
| title_short | Intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in RCS rats |
| title_sort | intravenous infusion of small umbilical cord mesenchymal stem cells could enhance safety and delay retinal degeneration in rcs rats |
| topic | Umbilical cord mesenchymal stem cells Intravenous infusion Safety Efficacy Retinitis pigmentosa |
| url | https://doi.org/10.1186/s12886-021-02171-3 |
| work_keys_str_mv | AT qinglingliang intravenousinfusionofsmallumbilicalcordmesenchymalstemcellscouldenhancesafetyanddelayretinaldegenerationinrcsrats AT qiyouli intravenousinfusionofsmallumbilicalcordmesenchymalstemcellscouldenhancesafetyanddelayretinaldegenerationinrcsrats AT bangqiren intravenousinfusionofsmallumbilicalcordmesenchymalstemcellscouldenhancesafetyanddelayretinaldegenerationinrcsrats AT zhengqinyin intravenousinfusionofsmallumbilicalcordmesenchymalstemcellscouldenhancesafetyanddelayretinaldegenerationinrcsrats |