Tissue-specific changes in Ca2+-ATPase and Na+/K+-ATPase activities in freshwater African catfish Clarias gariepinus juvenile exposed to oxadiazon
Abstract Background This study investigated the effect of sublethal concentrations (0.0, 0.3, 0.6, and 1.2 mg·L-1) of herbicide oxadiazon (ODZ) on the activities of Ca2+-ATPase and Na+/K+-ATPase in juvenile Clarias gariepinus. Methods One hundred eighty juveniles of Clarias gariepinus (mean weight 5...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-08-01
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| Series: | Journal of Basic and Applied Zoology |
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| Online Access: | http://link.springer.com/article/10.1186/s41936-020-00186-8 |
| _version_ | 1818319307858247680 |
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| author | Ndubuisi Stanley Oluah Bernard Obialo Mgbenka Christopher Didiugwu Nwani Ifeanyi Oscar Aguzie Innocent Chinedu Ngene Chidimma Oluah |
| author_facet | Ndubuisi Stanley Oluah Bernard Obialo Mgbenka Christopher Didiugwu Nwani Ifeanyi Oscar Aguzie Innocent Chinedu Ngene Chidimma Oluah |
| author_sort | Ndubuisi Stanley Oluah |
| collection | DOAJ |
| description | Abstract Background This study investigated the effect of sublethal concentrations (0.0, 0.3, 0.6, and 1.2 mg·L-1) of herbicide oxadiazon (ODZ) on the activities of Ca2+-ATPase and Na+/K+-ATPase in juvenile Clarias gariepinus. Methods One hundred eighty juveniles of Clarias gariepinus (mean weight 58.88 ± 1.24 g and mean length 22.34 ± 1.52 cm) were randomly divided into four groups and exposed to sublethal concentrations (0.00, 0.3, 0.6, and 1.2 mg·L-1 ODZ) for 21 days in a static renewal bioassay system in which the herbicide and water were replaced completely every day. The changes in Ca2+- and Na+/K+-ATPase activities in the gill, kidney, liver, and heart of the fish were assayed on days 1, 7, 14, and 21. Result The result showed significant alteration in the activity of these membrane-bound enzymes. There was duration and concentration-dependent significant (p < 0.05) increase in Ca2+-ATPase activity in the treatment groups when compared with the control. The Na+/K+-ATPase activity was significantly (p < 0.05) inhibited in all the tissues when compared with control. The observed alterations in the activities of both Ca2+-ATPase and Na+/K+-ATPase in this study may be indication of impaired ionic transport and imbalance in the fish which may trigger other biochemical, physiological, and even neurological consequences that may compromise several body functions. Conclusion The alteration of the ATPase activities in C. gariepinus by ODZ is likely to affect the ATP usage and energy metabolism in the fish serious repercussions on Ca2+ homeostasis, Na+/K+ sodium pump, and Ca2+/Na+ exchanger The results suggested that assay of the enzymes could be used as a biomarker of water pollution. |
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| format | Article |
| id | doaj.art-4a2167dc2f7047c3b8b1d25bd1a1dc84 |
| institution | Directory Open Access Journal |
| issn | 2090-990X |
| language | English |
| last_indexed | 2024-12-13T10:07:02Z |
| publishDate | 2020-08-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Journal of Basic and Applied Zoology |
| spelling | doaj.art-4a2167dc2f7047c3b8b1d25bd1a1dc842022-12-21T23:51:32ZengSpringerOpenJournal of Basic and Applied Zoology2090-990X2020-08-0181111210.1186/s41936-020-00186-8Tissue-specific changes in Ca2+-ATPase and Na+/K+-ATPase activities in freshwater African catfish Clarias gariepinus juvenile exposed to oxadiazonNdubuisi Stanley Oluah0Bernard Obialo Mgbenka1Christopher Didiugwu Nwani2Ifeanyi Oscar Aguzie3Innocent Chinedu Ngene4Chidimma Oluah5Ecotoxicology Research Unit, Aquaculture and Marine Science Programme, Department of Zoology and Environmental Biology, University of NigeriaEcotoxicology Research Unit, Aquaculture and Marine Science Programme, Department of Zoology and Environmental Biology, University of NigeriaEcotoxicology Research Unit, Aquaculture and Marine Science Programme, Department of Zoology and Environmental Biology, University of NigeriaEcotoxicology Research Unit, Aquaculture and Marine Science Programme, Department of Zoology and Environmental Biology, University of NigeriaEcotoxicology Research Unit, Aquaculture and Marine Science Programme, Department of Zoology and Environmental Biology, University of NigeriaDepartment of Medical Biochemistry, College of Medicine, University of NigeriaAbstract Background This study investigated the effect of sublethal concentrations (0.0, 0.3, 0.6, and 1.2 mg·L-1) of herbicide oxadiazon (ODZ) on the activities of Ca2+-ATPase and Na+/K+-ATPase in juvenile Clarias gariepinus. Methods One hundred eighty juveniles of Clarias gariepinus (mean weight 58.88 ± 1.24 g and mean length 22.34 ± 1.52 cm) were randomly divided into four groups and exposed to sublethal concentrations (0.00, 0.3, 0.6, and 1.2 mg·L-1 ODZ) for 21 days in a static renewal bioassay system in which the herbicide and water were replaced completely every day. The changes in Ca2+- and Na+/K+-ATPase activities in the gill, kidney, liver, and heart of the fish were assayed on days 1, 7, 14, and 21. Result The result showed significant alteration in the activity of these membrane-bound enzymes. There was duration and concentration-dependent significant (p < 0.05) increase in Ca2+-ATPase activity in the treatment groups when compared with the control. The Na+/K+-ATPase activity was significantly (p < 0.05) inhibited in all the tissues when compared with control. The observed alterations in the activities of both Ca2+-ATPase and Na+/K+-ATPase in this study may be indication of impaired ionic transport and imbalance in the fish which may trigger other biochemical, physiological, and even neurological consequences that may compromise several body functions. Conclusion The alteration of the ATPase activities in C. gariepinus by ODZ is likely to affect the ATP usage and energy metabolism in the fish serious repercussions on Ca2+ homeostasis, Na+/K+ sodium pump, and Ca2+/Na+ exchanger The results suggested that assay of the enzymes could be used as a biomarker of water pollution.http://link.springer.com/article/10.1186/s41936-020-00186-8ClariasHerbicideCa2+-ATPaseNa+/K+-ATPaseIonic imbalanceOsmoregulation |
| spellingShingle | Ndubuisi Stanley Oluah Bernard Obialo Mgbenka Christopher Didiugwu Nwani Ifeanyi Oscar Aguzie Innocent Chinedu Ngene Chidimma Oluah Tissue-specific changes in Ca2+-ATPase and Na+/K+-ATPase activities in freshwater African catfish Clarias gariepinus juvenile exposed to oxadiazon Journal of Basic and Applied Zoology Clarias Herbicide Ca2+-ATPase Na+/K+-ATPase Ionic imbalance Osmoregulation |
| title | Tissue-specific changes in Ca2+-ATPase and Na+/K+-ATPase activities in freshwater African catfish Clarias gariepinus juvenile exposed to oxadiazon |
| title_full | Tissue-specific changes in Ca2+-ATPase and Na+/K+-ATPase activities in freshwater African catfish Clarias gariepinus juvenile exposed to oxadiazon |
| title_fullStr | Tissue-specific changes in Ca2+-ATPase and Na+/K+-ATPase activities in freshwater African catfish Clarias gariepinus juvenile exposed to oxadiazon |
| title_full_unstemmed | Tissue-specific changes in Ca2+-ATPase and Na+/K+-ATPase activities in freshwater African catfish Clarias gariepinus juvenile exposed to oxadiazon |
| title_short | Tissue-specific changes in Ca2+-ATPase and Na+/K+-ATPase activities in freshwater African catfish Clarias gariepinus juvenile exposed to oxadiazon |
| title_sort | tissue specific changes in ca2 atpase and na k atpase activities in freshwater african catfish clarias gariepinus juvenile exposed to oxadiazon |
| topic | Clarias Herbicide Ca2+-ATPase Na+/K+-ATPase Ionic imbalance Osmoregulation |
| url | http://link.springer.com/article/10.1186/s41936-020-00186-8 |
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