The 22nd Chromatography Component of the <i>Fasciola gigantica</i> Excretory-Secretory Products Decreased the Proliferation of Peripheral Blood Mononuclear Cells from Buffalo
The 22nd chromatography component (F22) of the <i>Fasciola gigantica</i> excretory-secretory products (<i>Fg</i>ESP) shows better diagnostic value than the <i>Fg</i>ESP, and diagnostic methods based on F22 have also been established. Thus, exploring its immunomodu...
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| Format: | Article |
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MDPI AG
2023-02-01
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| Series: | Animals |
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| Online Access: | https://www.mdpi.com/2076-2615/13/4/564 |
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| author | Xiangxiang Yuan Xiaoge Han Xinping Kong Linjing Hou Kelong Wei Mingtang Chen Weiyu Zhang Wenda Di |
| author_facet | Xiangxiang Yuan Xiaoge Han Xinping Kong Linjing Hou Kelong Wei Mingtang Chen Weiyu Zhang Wenda Di |
| author_sort | Xiangxiang Yuan |
| collection | DOAJ |
| description | The 22nd chromatography component (F22) of the <i>Fasciola gigantica</i> excretory-secretory products (<i>Fg</i>ESP) shows better diagnostic value than the <i>Fg</i>ESP, and diagnostic methods based on F22 have also been established. Thus, exploring its immunomodulatory function and potential as a molecular vaccine candidate is attractive. In the present study, the effect of F22 on the mitogen-induced proliferation of buffalo peripheral blood mononuclear cells (PBMCs) in the innate immune response was preliminarily studied using the <i>Fg</i>ESP as a control. PBMCs were incubated with concanavalin A (ConA) and phytohemagglutinin (PHA) at optimal (1 µg/well) or suboptimal (0.25 µg/well) doses coupled with <i>Fg</i>ESP and F22 at different doses (1–16 µg/well). Cell proliferation was then assessed by microenzyme reaction colorimetry (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay). In addition, the components of F22 were also explored by mass spectrometry and then subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis to infer their functions. The results indicated that <i>Fg</i>ESP decreased the proliferation of PBMCs stimulated with ConA and PHA at specific doses, whereas F22 significantly decreased the proliferation of PBMCs stimulated with ConA and PHA at both optimal and suboptimal doses (<i>p</i> < 0.05). Two hundred and sixteen proteins were identified in F22, and these included 86 proteins that could be assigned to more than one pathway and some with robust immunomodulatory ability. Further studies should be performed to investigate the immunomodulatory function of F22 in the adaptive immune response, and the components of F22 can be further studied as potential vaccine candidate molecules. |
| first_indexed | 2024-03-11T09:15:54Z |
| format | Article |
| id | doaj.art-4a266112841c47559085be9cc107c068 |
| institution | Directory Open Access Journal |
| issn | 2076-2615 |
| language | English |
| last_indexed | 2024-03-11T09:15:54Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Animals |
| spelling | doaj.art-4a266112841c47559085be9cc107c0682023-11-16T18:38:20ZengMDPI AGAnimals2076-26152023-02-0113456410.3390/ani13040564The 22nd Chromatography Component of the <i>Fasciola gigantica</i> Excretory-Secretory Products Decreased the Proliferation of Peripheral Blood Mononuclear Cells from BuffaloXiangxiang Yuan0Xiaoge Han1Xinping Kong2Linjing Hou3Kelong Wei4Mingtang Chen5Weiyu Zhang6Wenda Di7College of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaGuangxi Buffalo Research Institute, Chinese Academy Agricultural Sciences, Nanning 530001, ChinaGuangxi Buffalo Research Institute, Chinese Academy Agricultural Sciences, Nanning 530001, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaCollege of Animal Science and Technology, Guangxi University, Nanning 530004, ChinaThe 22nd chromatography component (F22) of the <i>Fasciola gigantica</i> excretory-secretory products (<i>Fg</i>ESP) shows better diagnostic value than the <i>Fg</i>ESP, and diagnostic methods based on F22 have also been established. Thus, exploring its immunomodulatory function and potential as a molecular vaccine candidate is attractive. In the present study, the effect of F22 on the mitogen-induced proliferation of buffalo peripheral blood mononuclear cells (PBMCs) in the innate immune response was preliminarily studied using the <i>Fg</i>ESP as a control. PBMCs were incubated with concanavalin A (ConA) and phytohemagglutinin (PHA) at optimal (1 µg/well) or suboptimal (0.25 µg/well) doses coupled with <i>Fg</i>ESP and F22 at different doses (1–16 µg/well). Cell proliferation was then assessed by microenzyme reaction colorimetry (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium (MTT) assay). In addition, the components of F22 were also explored by mass spectrometry and then subjected to Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis to infer their functions. The results indicated that <i>Fg</i>ESP decreased the proliferation of PBMCs stimulated with ConA and PHA at specific doses, whereas F22 significantly decreased the proliferation of PBMCs stimulated with ConA and PHA at both optimal and suboptimal doses (<i>p</i> < 0.05). Two hundred and sixteen proteins were identified in F22, and these included 86 proteins that could be assigned to more than one pathway and some with robust immunomodulatory ability. Further studies should be performed to investigate the immunomodulatory function of F22 in the adaptive immune response, and the components of F22 can be further studied as potential vaccine candidate molecules.https://www.mdpi.com/2076-2615/13/4/564<i>Fasciola gigantica</i>Buffalocell proliferationimmunomodulatoryPBMCs |
| spellingShingle | Xiangxiang Yuan Xiaoge Han Xinping Kong Linjing Hou Kelong Wei Mingtang Chen Weiyu Zhang Wenda Di The 22nd Chromatography Component of the <i>Fasciola gigantica</i> Excretory-Secretory Products Decreased the Proliferation of Peripheral Blood Mononuclear Cells from Buffalo Animals <i>Fasciola gigantica</i> Buffalo cell proliferation immunomodulatory PBMCs |
| title | The 22nd Chromatography Component of the <i>Fasciola gigantica</i> Excretory-Secretory Products Decreased the Proliferation of Peripheral Blood Mononuclear Cells from Buffalo |
| title_full | The 22nd Chromatography Component of the <i>Fasciola gigantica</i> Excretory-Secretory Products Decreased the Proliferation of Peripheral Blood Mononuclear Cells from Buffalo |
| title_fullStr | The 22nd Chromatography Component of the <i>Fasciola gigantica</i> Excretory-Secretory Products Decreased the Proliferation of Peripheral Blood Mononuclear Cells from Buffalo |
| title_full_unstemmed | The 22nd Chromatography Component of the <i>Fasciola gigantica</i> Excretory-Secretory Products Decreased the Proliferation of Peripheral Blood Mononuclear Cells from Buffalo |
| title_short | The 22nd Chromatography Component of the <i>Fasciola gigantica</i> Excretory-Secretory Products Decreased the Proliferation of Peripheral Blood Mononuclear Cells from Buffalo |
| title_sort | 22nd chromatography component of the i fasciola gigantica i excretory secretory products decreased the proliferation of peripheral blood mononuclear cells from buffalo |
| topic | <i>Fasciola gigantica</i> Buffalo cell proliferation immunomodulatory PBMCs |
| url | https://www.mdpi.com/2076-2615/13/4/564 |
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