The Influence of Gastric Antral Ulcerations on the Expression of Galanin and GalR1, GalR2, GalR3 Receptors in the Pylorus with Regard to Gastric Intrinsic Innervation of the Pyloric Sphincter.
Gastric antrum ulcerations are common disorders occurring in humans and animals. Such localization of ulcers disturbs the gastric emptying process, which is precisely controlled by the pylorus. Galanin (Gal) and its receptors are commonly accepted to participate in the regulation of inflammatory pro...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2016-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4866767?pdf=render |
| _version_ | 1828880895527878656 |
|---|---|
| author | Michal Zalecki Waldemar Sienkiewicz Amelia Franke-Radowiecka Magdalena Klimczuk Jerzy Kaleczyc |
| author_facet | Michal Zalecki Waldemar Sienkiewicz Amelia Franke-Radowiecka Magdalena Klimczuk Jerzy Kaleczyc |
| author_sort | Michal Zalecki |
| collection | DOAJ |
| description | Gastric antrum ulcerations are common disorders occurring in humans and animals. Such localization of ulcers disturbs the gastric emptying process, which is precisely controlled by the pylorus. Galanin (Gal) and its receptors are commonly accepted to participate in the regulation of inflammatory processes and neuronal plasticity. Their role in the regulation of gastrointestinal motility is also widely described. However, there is lack of data considering antral ulcerations in relation to changes in the expression of Gal and GalR1, GalR2, GalR3 receptors in the pyloric wall tissue and galaninergic intramural innervation of the pylorus. Two groups of pigs were used in the study: healthy gilts and gilts with experimentally induced antral ulcers. By double immunocytochemistry percentages of myenteric and submucosal neurons expressing Gal-immunoreactivity were determined in the pyloric wall tissue and in the population of gastric descending neurons supplying the pyloric sphincter (labelled by retrograde Fast Blue neuronal tracer). The percentage of Gal-immunoreactive neurons increased only in the myenteric plexus of the pyloric wall (from 16.14±2.06% in control to 25.5±2.07% in experimental animals), while no significant differences in other neuronal populations were observed between animals of both groups. Real-Time PCR revealed the increased expression of mRNA encoding Gal and GalR1 receptor in the pyloric wall tissue of the experimental animals, while the expression(s) of GalR2 and GalR3 were not significantly changed. The results obtained suggest the involvement of Gal, GalR1 and galaninergic pyloric myenteric neurons in the response of pyloric wall structures to antral ulcerations. |
| first_indexed | 2024-12-13T09:57:46Z |
| format | Article |
| id | doaj.art-4a2a689229c647b1921faf4623724369 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T09:57:46Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-4a2a689229c647b1921faf46237243692022-12-21T23:51:45ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01115e015565810.1371/journal.pone.0155658The Influence of Gastric Antral Ulcerations on the Expression of Galanin and GalR1, GalR2, GalR3 Receptors in the Pylorus with Regard to Gastric Intrinsic Innervation of the Pyloric Sphincter.Michal ZaleckiWaldemar SienkiewiczAmelia Franke-RadowieckaMagdalena KlimczukJerzy KaleczycGastric antrum ulcerations are common disorders occurring in humans and animals. Such localization of ulcers disturbs the gastric emptying process, which is precisely controlled by the pylorus. Galanin (Gal) and its receptors are commonly accepted to participate in the regulation of inflammatory processes and neuronal plasticity. Their role in the regulation of gastrointestinal motility is also widely described. However, there is lack of data considering antral ulcerations in relation to changes in the expression of Gal and GalR1, GalR2, GalR3 receptors in the pyloric wall tissue and galaninergic intramural innervation of the pylorus. Two groups of pigs were used in the study: healthy gilts and gilts with experimentally induced antral ulcers. By double immunocytochemistry percentages of myenteric and submucosal neurons expressing Gal-immunoreactivity were determined in the pyloric wall tissue and in the population of gastric descending neurons supplying the pyloric sphincter (labelled by retrograde Fast Blue neuronal tracer). The percentage of Gal-immunoreactive neurons increased only in the myenteric plexus of the pyloric wall (from 16.14±2.06% in control to 25.5±2.07% in experimental animals), while no significant differences in other neuronal populations were observed between animals of both groups. Real-Time PCR revealed the increased expression of mRNA encoding Gal and GalR1 receptor in the pyloric wall tissue of the experimental animals, while the expression(s) of GalR2 and GalR3 were not significantly changed. The results obtained suggest the involvement of Gal, GalR1 and galaninergic pyloric myenteric neurons in the response of pyloric wall structures to antral ulcerations.http://europepmc.org/articles/PMC4866767?pdf=render |
| spellingShingle | Michal Zalecki Waldemar Sienkiewicz Amelia Franke-Radowiecka Magdalena Klimczuk Jerzy Kaleczyc The Influence of Gastric Antral Ulcerations on the Expression of Galanin and GalR1, GalR2, GalR3 Receptors in the Pylorus with Regard to Gastric Intrinsic Innervation of the Pyloric Sphincter. PLoS ONE |
| title | The Influence of Gastric Antral Ulcerations on the Expression of Galanin and GalR1, GalR2, GalR3 Receptors in the Pylorus with Regard to Gastric Intrinsic Innervation of the Pyloric Sphincter. |
| title_full | The Influence of Gastric Antral Ulcerations on the Expression of Galanin and GalR1, GalR2, GalR3 Receptors in the Pylorus with Regard to Gastric Intrinsic Innervation of the Pyloric Sphincter. |
| title_fullStr | The Influence of Gastric Antral Ulcerations on the Expression of Galanin and GalR1, GalR2, GalR3 Receptors in the Pylorus with Regard to Gastric Intrinsic Innervation of the Pyloric Sphincter. |
| title_full_unstemmed | The Influence of Gastric Antral Ulcerations on the Expression of Galanin and GalR1, GalR2, GalR3 Receptors in the Pylorus with Regard to Gastric Intrinsic Innervation of the Pyloric Sphincter. |
| title_short | The Influence of Gastric Antral Ulcerations on the Expression of Galanin and GalR1, GalR2, GalR3 Receptors in the Pylorus with Regard to Gastric Intrinsic Innervation of the Pyloric Sphincter. |
| title_sort | influence of gastric antral ulcerations on the expression of galanin and galr1 galr2 galr3 receptors in the pylorus with regard to gastric intrinsic innervation of the pyloric sphincter |
| url | http://europepmc.org/articles/PMC4866767?pdf=render |
| work_keys_str_mv | AT michalzalecki theinfluenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter AT waldemarsienkiewicz theinfluenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter AT ameliafrankeradowiecka theinfluenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter AT magdalenaklimczuk theinfluenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter AT jerzykaleczyc theinfluenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter AT michalzalecki influenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter AT waldemarsienkiewicz influenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter AT ameliafrankeradowiecka influenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter AT magdalenaklimczuk influenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter AT jerzykaleczyc influenceofgastricantralulcerationsontheexpressionofgalaninandgalr1galr2galr3receptorsinthepyloruswithregardtogastricintrinsicinnervationofthepyloricsphincter |