Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis
Background: One of the consequences of toxoplasmosis is the risk of passing it from mother to fetus and the onset of congenital toxoplasmosis during pregnancy. The purpose of this study was to evaluate the B1 gene of Toxoplasma gondii in the placental tissues of pregnant women with acute toxoplasmos...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2018-01-01
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| Series: | Advanced Biomedical Research |
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| Online Access: | http://www.advbiores.net/article.asp?issn=2277-9175;year=2018;volume=7;issue=1;spage=119;epage=119;aulast=Sardarian |
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| author | Khosro Sardarian Amir Hossein Maghsood Marzieh Farimani Mehrdad Hajiloii Massoud Saidijam Mahsa Rezaeepoor Hanie Mahaki Alireza Zamani |
| author_facet | Khosro Sardarian Amir Hossein Maghsood Marzieh Farimani Mehrdad Hajiloii Massoud Saidijam Mahsa Rezaeepoor Hanie Mahaki Alireza Zamani |
| author_sort | Khosro Sardarian |
| collection | DOAJ |
| description | Background: One of the consequences of toxoplasmosis is the risk of passing it from mother to fetus and the onset of congenital toxoplasmosis during pregnancy. The purpose of this study was to evaluate the B1 gene of Toxoplasma gondii in the placental tissues of pregnant women with acute toxoplasmosis. Materials and Methods: The study was a cross-sectional study. Serum samples of pregnant women who attended to Fatemieh Hospital of Hamadan University of Medical Sciences were tested for immunoglobulin G (IgG) antibodies against T. gondii by enzyme-linked immunosorbent assay. Then, polymerase chain reaction was used to identify the specific B1 gene of T. gondii in IgG seropositive women. The placental tissues of the pregnant women with positive serum B1 gene examined for this gene. Anti-Toxoplasma immunoglobulin M (IgM) was performed on the umbilical cord and neonate blood. Results: Anti-Toxoplasma IgG was detected in 167 out of 653 (25.6%) pregnant women. T. gondii B1 gene was identified in 36 out of 167 (21.6%) of IgG seropositive women. After delivery, the B1 gene was evaluated in 15 out of 36 (41.7%) patients' placental tissues, 13 of which were positive for this gene (86.7%). Anti-Toxoplasma IgM was detected neither in any umbilical cord nor in neonatal blood samples. All newborns, with the exception of one case, were born with normal birth weight and in term birth. Conclusion: The B1 gene was detected in 86.7% of the placental tissue of women who were involved in acute toxoplasmosis during pregnancy. |
| first_indexed | 2024-04-13T14:02:03Z |
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| id | doaj.art-4a2ad5af3bfb46da9220abb9ff68a6e1 |
| institution | Directory Open Access Journal |
| issn | 2277-9175 |
| language | English |
| last_indexed | 2024-04-13T14:02:03Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Advanced Biomedical Research |
| spelling | doaj.art-4a2ad5af3bfb46da9220abb9ff68a6e12022-12-22T02:44:01ZengWolters Kluwer Medknow PublicationsAdvanced Biomedical Research2277-91752018-01-017111911910.4103/abr.abr_58_18Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute ToxoplasmosisKhosro SardarianAmir Hossein MaghsoodMarzieh FarimaniMehrdad HajiloiiMassoud SaidijamMahsa RezaeepoorHanie MahakiAlireza ZamaniBackground: One of the consequences of toxoplasmosis is the risk of passing it from mother to fetus and the onset of congenital toxoplasmosis during pregnancy. The purpose of this study was to evaluate the B1 gene of Toxoplasma gondii in the placental tissues of pregnant women with acute toxoplasmosis. Materials and Methods: The study was a cross-sectional study. Serum samples of pregnant women who attended to Fatemieh Hospital of Hamadan University of Medical Sciences were tested for immunoglobulin G (IgG) antibodies against T. gondii by enzyme-linked immunosorbent assay. Then, polymerase chain reaction was used to identify the specific B1 gene of T. gondii in IgG seropositive women. The placental tissues of the pregnant women with positive serum B1 gene examined for this gene. Anti-Toxoplasma immunoglobulin M (IgM) was performed on the umbilical cord and neonate blood. Results: Anti-Toxoplasma IgG was detected in 167 out of 653 (25.6%) pregnant women. T. gondii B1 gene was identified in 36 out of 167 (21.6%) of IgG seropositive women. After delivery, the B1 gene was evaluated in 15 out of 36 (41.7%) patients' placental tissues, 13 of which were positive for this gene (86.7%). Anti-Toxoplasma IgM was detected neither in any umbilical cord nor in neonatal blood samples. All newborns, with the exception of one case, were born with normal birth weight and in term birth. Conclusion: The B1 gene was detected in 86.7% of the placental tissue of women who were involved in acute toxoplasmosis during pregnancy.http://www.advbiores.net/article.asp?issn=2277-9175;year=2018;volume=7;issue=1;spage=119;epage=119;aulast=SardarianPlacentapolymerase chain reactiontoxoplasmosis |
| spellingShingle | Khosro Sardarian Amir Hossein Maghsood Marzieh Farimani Mehrdad Hajiloii Massoud Saidijam Mahsa Rezaeepoor Hanie Mahaki Alireza Zamani Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis Advanced Biomedical Research Placenta polymerase chain reaction toxoplasmosis |
| title | Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis |
| title_full | Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis |
| title_fullStr | Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis |
| title_full_unstemmed | Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis |
| title_short | Evaluation of Toxoplasma gondii B1 gene in Placental Tissues of Pregnant Women with Acute Toxoplasmosis |
| title_sort | evaluation of toxoplasma gondii b1 gene in placental tissues of pregnant women with acute toxoplasmosis |
| topic | Placenta polymerase chain reaction toxoplasmosis |
| url | http://www.advbiores.net/article.asp?issn=2277-9175;year=2018;volume=7;issue=1;spage=119;epage=119;aulast=Sardarian |
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