Taking the stock of granule cargo: Platelet releasate proteomics
Human platelets are key players in a multitude of physiological and pathological processes. Upon activation they release cargo from different types of granules as well as microparticles in an apparently well-regulated and orchestrated manner. The resulting specific platelet releasates create microen...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2017-02-01
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| Series: | Platelets |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09537104.2016.1254762 |
| _version_ | 1797684260447977472 |
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| author | Oliver Pagel Elena Walter Kerstin Jurk René P. Zahedi |
| author_facet | Oliver Pagel Elena Walter Kerstin Jurk René P. Zahedi |
| author_sort | Oliver Pagel |
| collection | DOAJ |
| description | Human platelets are key players in a multitude of physiological and pathological processes. Upon activation they release cargo from different types of granules as well as microparticles in an apparently well-regulated and orchestrated manner. The resulting specific platelet releasates create microenvironments of biologically active compounds and proteins during platelet aggregation and thrombus formation, allowing efficient delivery of growth factors and immune modulators to their sites of effect and enhancing the coagulative response in a positive feedback loop. Thus, platelet releasates play a central role in the regulation of platelet homeostasis and heterotypic cell interaction. Additionally, it recently emerged that both the qualitative and quantitative composition of the releasate as well as release dynamics may be stimulus dependent and therefore more complex than expected. Mass spectrometry-based proteomics is an important asset for studying platelet releasates in vitro, as it allows not only (i) identifying released proteins, but moreover (ii) determining their quantities and the dynamics of release as well as (iii) differentially comparing releasates across a variety of conditions. Though owing to the high sensitivity and comprehensiveness of modern proteomic techniques, a thorough experimental design and a standardized and robust sample preparation are essential to obtain highly confident and reliable insights into platelet biology and pathology. Here, we review releasate proteome studies and crucial sample preparation strategies to summarize possible achievements of state-of-the-art technologies and furthermore discuss potential pitfalls and limitations. We provide a future perspective of platelet releasate proteomics including targeted analyses, post-translational modifications and multi-omics approaches that should be adopted by platelet releasate researchers due to their tremendous depth and comprehensiveness. |
| first_indexed | 2024-03-12T00:28:04Z |
| format | Article |
| id | doaj.art-4a2be5b99f334803a72e0acf18a590ac |
| institution | Directory Open Access Journal |
| issn | 0953-7104 1369-1635 |
| language | English |
| last_indexed | 2024-03-12T00:28:04Z |
| publishDate | 2017-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Platelets |
| spelling | doaj.art-4a2be5b99f334803a72e0acf18a590ac2023-09-15T10:31:57ZengTaylor & Francis GroupPlatelets0953-71041369-16352017-02-0128211912810.1080/09537104.2016.12547621254762Taking the stock of granule cargo: Platelet releasate proteomicsOliver Pagel0Elena Walter1Kerstin Jurk2René P. Zahedi3Leibniz-Institut für Analytische Wissenschaften – ISAS – e.VUniversitätsklinikum der Johannes Gutenberg-Universität MainzUniversitätsklinikum der Johannes Gutenberg-Universität MainzLeibniz-Institut für Analytische Wissenschaften – ISAS – e.VHuman platelets are key players in a multitude of physiological and pathological processes. Upon activation they release cargo from different types of granules as well as microparticles in an apparently well-regulated and orchestrated manner. The resulting specific platelet releasates create microenvironments of biologically active compounds and proteins during platelet aggregation and thrombus formation, allowing efficient delivery of growth factors and immune modulators to their sites of effect and enhancing the coagulative response in a positive feedback loop. Thus, platelet releasates play a central role in the regulation of platelet homeostasis and heterotypic cell interaction. Additionally, it recently emerged that both the qualitative and quantitative composition of the releasate as well as release dynamics may be stimulus dependent and therefore more complex than expected. Mass spectrometry-based proteomics is an important asset for studying platelet releasates in vitro, as it allows not only (i) identifying released proteins, but moreover (ii) determining their quantities and the dynamics of release as well as (iii) differentially comparing releasates across a variety of conditions. Though owing to the high sensitivity and comprehensiveness of modern proteomic techniques, a thorough experimental design and a standardized and robust sample preparation are essential to obtain highly confident and reliable insights into platelet biology and pathology. Here, we review releasate proteome studies and crucial sample preparation strategies to summarize possible achievements of state-of-the-art technologies and furthermore discuss potential pitfalls and limitations. We provide a future perspective of platelet releasate proteomics including targeted analyses, post-translational modifications and multi-omics approaches that should be adopted by platelet releasate researchers due to their tremendous depth and comprehensiveness.http://dx.doi.org/10.1080/09537104.2016.1254762platelet releasategranule cargolc-ms/ms based proteomicssample preparation |
| spellingShingle | Oliver Pagel Elena Walter Kerstin Jurk René P. Zahedi Taking the stock of granule cargo: Platelet releasate proteomics Platelets platelet releasate granule cargo lc-ms/ms based proteomics sample preparation |
| title | Taking the stock of granule cargo: Platelet releasate proteomics |
| title_full | Taking the stock of granule cargo: Platelet releasate proteomics |
| title_fullStr | Taking the stock of granule cargo: Platelet releasate proteomics |
| title_full_unstemmed | Taking the stock of granule cargo: Platelet releasate proteomics |
| title_short | Taking the stock of granule cargo: Platelet releasate proteomics |
| title_sort | taking the stock of granule cargo platelet releasate proteomics |
| topic | platelet releasate granule cargo lc-ms/ms based proteomics sample preparation |
| url | http://dx.doi.org/10.1080/09537104.2016.1254762 |
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