Genome Analysis of <i>Thinopyrum intermedium</i> and Its Potential Progenitor Species Using Oligo-FISH
The genome composition of intermediate wheatgrass (IWG) is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of <i>Th. intermedium</i> and its relative diploid species. St<sub>2</sub&g...
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MDPI AG
2023-10-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/12/21/3705 |
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| author | Fei Qi Shuang Liang Piyi Xing Yinguang Bao Richard R.-C. Wang Xingfeng Li |
| author_facet | Fei Qi Shuang Liang Piyi Xing Yinguang Bao Richard R.-C. Wang Xingfeng Li |
| author_sort | Fei Qi |
| collection | DOAJ |
| description | The genome composition of intermediate wheatgrass (IWG) is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of <i>Th. intermedium</i> and its relative diploid species. St<sub>2</sub>-80 developed from <i>Pseudowroegneria strigose</i> and pDb12H developed from <i>Dasypyrum breviaristatum</i> were used as probes in fluorescence in situ hybridization (FISH) to classify the chromosomes of <i>Th. intermedium</i> into three groups, expressed as <b>J<sup>vs</sup>J<sup>vs</sup>J<sup>r</sup>J<sup>r</sup>StSt</b>. A combined multiplex oligonucleotide probe, including pSc119.2-1, (GAA)<sub>10</sub>, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6, was used to establish the FISH karyotype of ten accessions of <i>Th. intermedium</i>. Variability among and within the studied accessions of intermediate wheatgrass was observed in their FISH patterns. Results of this study led to the conclusions that <b>J<sup>vs</sup></b> had largely been contributed from <i>Da. breviaristatum</i>, but not the present-day <i>Da. villosum</i>; IWG had only one <b>J</b> genome, <b>J<sup>r</sup></b>, which was related to either <i>Th. elongatum</i> or <i>Th. bessarabicum</i>; and <b>St</b> was contributed from the genus <i>Pseudoroegneria</i> by hybridization with <i>Th. junceiforme</i> or <i>Th. sartorii</i>. |
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| last_indexed | 2024-03-11T11:23:18Z |
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| spelling | doaj.art-4a31e729798b4da4b9805aa6c07ff1ea2023-11-10T15:10:14ZengMDPI AGPlants2223-77472023-10-011221370510.3390/plants12213705Genome Analysis of <i>Thinopyrum intermedium</i> and Its Potential Progenitor Species Using Oligo-FISHFei Qi0Shuang Liang1Piyi Xing2Yinguang Bao3Richard R.-C. Wang4Xingfeng Li5State Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaAgronomy College, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaUSDA-ARS Forage & Range Research Laboratory (FRRL), Logan, UT 84322-6300, USAState Key Laboratory of Crop Biology, Shandong Agricultural University, Tai’an 271018, ChinaThe genome composition of intermediate wheatgrass (IWG) is complex and continues to be a subject of investigation. In this study, molecular cytogenetics were used to investigate the karyotype composition of <i>Th. intermedium</i> and its relative diploid species. St<sub>2</sub>-80 developed from <i>Pseudowroegneria strigose</i> and pDb12H developed from <i>Dasypyrum breviaristatum</i> were used as probes in fluorescence in situ hybridization (FISH) to classify the chromosomes of <i>Th. intermedium</i> into three groups, expressed as <b>J<sup>vs</sup>J<sup>vs</sup>J<sup>r</sup>J<sup>r</sup>StSt</b>. A combined multiplex oligonucleotide probe, including pSc119.2-1, (GAA)<sub>10</sub>, AFA-3, AFA-4, pAs1-1, Pas1-3, pAs1-4, and pAs1-6, was used to establish the FISH karyotype of ten accessions of <i>Th. intermedium</i>. Variability among and within the studied accessions of intermediate wheatgrass was observed in their FISH patterns. Results of this study led to the conclusions that <b>J<sup>vs</sup></b> had largely been contributed from <i>Da. breviaristatum</i>, but not the present-day <i>Da. villosum</i>; IWG had only one <b>J</b> genome, <b>J<sup>r</sup></b>, which was related to either <i>Th. elongatum</i> or <i>Th. bessarabicum</i>; and <b>St</b> was contributed from the genus <i>Pseudoroegneria</i> by hybridization with <i>Th. junceiforme</i> or <i>Th. sartorii</i>.https://www.mdpi.com/2223-7747/12/21/3705Triticeaegenome constitutionFISHevolutionkaryotype |
| spellingShingle | Fei Qi Shuang Liang Piyi Xing Yinguang Bao Richard R.-C. Wang Xingfeng Li Genome Analysis of <i>Thinopyrum intermedium</i> and Its Potential Progenitor Species Using Oligo-FISH Plants Triticeae genome constitution FISH evolution karyotype |
| title | Genome Analysis of <i>Thinopyrum intermedium</i> and Its Potential Progenitor Species Using Oligo-FISH |
| title_full | Genome Analysis of <i>Thinopyrum intermedium</i> and Its Potential Progenitor Species Using Oligo-FISH |
| title_fullStr | Genome Analysis of <i>Thinopyrum intermedium</i> and Its Potential Progenitor Species Using Oligo-FISH |
| title_full_unstemmed | Genome Analysis of <i>Thinopyrum intermedium</i> and Its Potential Progenitor Species Using Oligo-FISH |
| title_short | Genome Analysis of <i>Thinopyrum intermedium</i> and Its Potential Progenitor Species Using Oligo-FISH |
| title_sort | genome analysis of i thinopyrum intermedium i and its potential progenitor species using oligo fish |
| topic | Triticeae genome constitution FISH evolution karyotype |
| url | https://www.mdpi.com/2223-7747/12/21/3705 |
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