Effects of nitric oxide inhibitors in mice with bladder outlet obstruction

ABSTRACT Purpose To investigate the lower urinary tract changes in mice treated with L-NAME, a non-selective competitive inhibitor of nitric oxide synthase (NOS), or aminoguanidine, a competitive inhibitor of inducible nitric oxide synthase (iNOS), after 5 weeks of partial bladder outlet obstructi...

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Main Authors: Marcy Lancia Pereira, Carlos Arturo Levi D’ancona, Julio Alejandro Rojas-Moscoso, Antonio Celso Saragossa Ramos Filho, Fabiola Zakia Mónica, Edson Antunes
Format: Article
Language:English
Published: Sociedade Brasileira de Urologia
Series:International Brazilian Journal of Urology
Subjects:
Online Access:http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382017000200356&lng=en&tlng=en
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author Marcy Lancia Pereira
Carlos Arturo Levi D’ancona
Julio Alejandro Rojas-Moscoso
Antonio Celso Saragossa Ramos Filho
Fabiola Zakia Mónica
Edson Antunes
author_facet Marcy Lancia Pereira
Carlos Arturo Levi D’ancona
Julio Alejandro Rojas-Moscoso
Antonio Celso Saragossa Ramos Filho
Fabiola Zakia Mónica
Edson Antunes
author_sort Marcy Lancia Pereira
collection DOAJ
description ABSTRACT Purpose To investigate the lower urinary tract changes in mice treated with L-NAME, a non-selective competitive inhibitor of nitric oxide synthase (NOS), or aminoguanidine, a competitive inhibitor of inducible nitric oxide synthase (iNOS), after 5 weeks of partial bladder outlet obstruction (BOO), in order to evaluate the role of constitutive and non-constitutive NOS in the pathogenesis of this experimental condition. Materials and Methods C57BL6 male mice were partially obstructed and randomly allocated into 6 groups: Sham, Sham + L-NAME, Sham + aminoguanidine, BOO, BOO + L-NAME and BOO + aminoguanidine. After 5 weeks, bladder weight was obtained and cystometry and tissue bath contractile studies were performed. Results BOO animals showed increase of non-voiding contractions (NVC) and bladder capacity, and also less contractile response to Carbachol and Electric Field Stimulation. Inhibition of NOS isoforms improved bladder capacity and compliance in BOO animals. L-NAME caused more NVC, prevented bladder weight gain and leaded to augmented contractile responses at muscarinic and electric stimulation. Aminoguanidine diminished NVC, but did not avoid bladder weight gain in BOO animals and did not improve contractile responses. Conclusion It can be hypothesized that chronic inhibition of three NOS isoforms in BOO animals leaded to worsening of bladder function, while selective inhibition of iNOS did not improve responses, what suggests that, in BOO animals, alterations are related to constitutive NOS.
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spelling doaj.art-4a320d338de84c83b01721fb83d50ed52022-12-21T16:58:21ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-611943235636610.1590/s1677-5538.ibju.2015.0441S1677-55382017000200356Effects of nitric oxide inhibitors in mice with bladder outlet obstructionMarcy Lancia PereiraCarlos Arturo Levi D’anconaJulio Alejandro Rojas-MoscosoAntonio Celso Saragossa Ramos FilhoFabiola Zakia MónicaEdson AntunesABSTRACT Purpose To investigate the lower urinary tract changes in mice treated with L-NAME, a non-selective competitive inhibitor of nitric oxide synthase (NOS), or aminoguanidine, a competitive inhibitor of inducible nitric oxide synthase (iNOS), after 5 weeks of partial bladder outlet obstruction (BOO), in order to evaluate the role of constitutive and non-constitutive NOS in the pathogenesis of this experimental condition. Materials and Methods C57BL6 male mice were partially obstructed and randomly allocated into 6 groups: Sham, Sham + L-NAME, Sham + aminoguanidine, BOO, BOO + L-NAME and BOO + aminoguanidine. After 5 weeks, bladder weight was obtained and cystometry and tissue bath contractile studies were performed. Results BOO animals showed increase of non-voiding contractions (NVC) and bladder capacity, and also less contractile response to Carbachol and Electric Field Stimulation. Inhibition of NOS isoforms improved bladder capacity and compliance in BOO animals. L-NAME caused more NVC, prevented bladder weight gain and leaded to augmented contractile responses at muscarinic and electric stimulation. Aminoguanidine diminished NVC, but did not avoid bladder weight gain in BOO animals and did not improve contractile responses. Conclusion It can be hypothesized that chronic inhibition of three NOS isoforms in BOO animals leaded to worsening of bladder function, while selective inhibition of iNOS did not improve responses, what suggests that, in BOO animals, alterations are related to constitutive NOS.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382017000200356&lng=en&tlng=enNG-Nitroarginine Methyl EsterNitric OxideUrinary BladderUreteral Obstruction
spellingShingle Marcy Lancia Pereira
Carlos Arturo Levi D’ancona
Julio Alejandro Rojas-Moscoso
Antonio Celso Saragossa Ramos Filho
Fabiola Zakia Mónica
Edson Antunes
Effects of nitric oxide inhibitors in mice with bladder outlet obstruction
International Brazilian Journal of Urology
NG-Nitroarginine Methyl Ester
Nitric Oxide
Urinary Bladder
Ureteral Obstruction
title Effects of nitric oxide inhibitors in mice with bladder outlet obstruction
title_full Effects of nitric oxide inhibitors in mice with bladder outlet obstruction
title_fullStr Effects of nitric oxide inhibitors in mice with bladder outlet obstruction
title_full_unstemmed Effects of nitric oxide inhibitors in mice with bladder outlet obstruction
title_short Effects of nitric oxide inhibitors in mice with bladder outlet obstruction
title_sort effects of nitric oxide inhibitors in mice with bladder outlet obstruction
topic NG-Nitroarginine Methyl Ester
Nitric Oxide
Urinary Bladder
Ureteral Obstruction
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382017000200356&lng=en&tlng=en
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