Effects of nitric oxide inhibitors in mice with bladder outlet obstruction
ABSTRACT Purpose To investigate the lower urinary tract changes in mice treated with L-NAME, a non-selective competitive inhibitor of nitric oxide synthase (NOS), or aminoguanidine, a competitive inhibitor of inducible nitric oxide synthase (iNOS), after 5 weeks of partial bladder outlet obstructi...
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Language: | English |
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Sociedade Brasileira de Urologia
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Series: | International Brazilian Journal of Urology |
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Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382017000200356&lng=en&tlng=en |
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author | Marcy Lancia Pereira Carlos Arturo Levi D’ancona Julio Alejandro Rojas-Moscoso Antonio Celso Saragossa Ramos Filho Fabiola Zakia Mónica Edson Antunes |
author_facet | Marcy Lancia Pereira Carlos Arturo Levi D’ancona Julio Alejandro Rojas-Moscoso Antonio Celso Saragossa Ramos Filho Fabiola Zakia Mónica Edson Antunes |
author_sort | Marcy Lancia Pereira |
collection | DOAJ |
description | ABSTRACT Purpose To investigate the lower urinary tract changes in mice treated with L-NAME, a non-selective competitive inhibitor of nitric oxide synthase (NOS), or aminoguanidine, a competitive inhibitor of inducible nitric oxide synthase (iNOS), after 5 weeks of partial bladder outlet obstruction (BOO), in order to evaluate the role of constitutive and non-constitutive NOS in the pathogenesis of this experimental condition. Materials and Methods C57BL6 male mice were partially obstructed and randomly allocated into 6 groups: Sham, Sham + L-NAME, Sham + aminoguanidine, BOO, BOO + L-NAME and BOO + aminoguanidine. After 5 weeks, bladder weight was obtained and cystometry and tissue bath contractile studies were performed. Results BOO animals showed increase of non-voiding contractions (NVC) and bladder capacity, and also less contractile response to Carbachol and Electric Field Stimulation. Inhibition of NOS isoforms improved bladder capacity and compliance in BOO animals. L-NAME caused more NVC, prevented bladder weight gain and leaded to augmented contractile responses at muscarinic and electric stimulation. Aminoguanidine diminished NVC, but did not avoid bladder weight gain in BOO animals and did not improve contractile responses. Conclusion It can be hypothesized that chronic inhibition of three NOS isoforms in BOO animals leaded to worsening of bladder function, while selective inhibition of iNOS did not improve responses, what suggests that, in BOO animals, alterations are related to constitutive NOS. |
first_indexed | 2024-12-24T11:16:18Z |
format | Article |
id | doaj.art-4a320d338de84c83b01721fb83d50ed5 |
institution | Directory Open Access Journal |
issn | 1677-6119 |
language | English |
last_indexed | 2024-12-24T11:16:18Z |
publisher | Sociedade Brasileira de Urologia |
record_format | Article |
series | International Brazilian Journal of Urology |
spelling | doaj.art-4a320d338de84c83b01721fb83d50ed52022-12-21T16:58:21ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-611943235636610.1590/s1677-5538.ibju.2015.0441S1677-55382017000200356Effects of nitric oxide inhibitors in mice with bladder outlet obstructionMarcy Lancia PereiraCarlos Arturo Levi D’anconaJulio Alejandro Rojas-MoscosoAntonio Celso Saragossa Ramos FilhoFabiola Zakia MónicaEdson AntunesABSTRACT Purpose To investigate the lower urinary tract changes in mice treated with L-NAME, a non-selective competitive inhibitor of nitric oxide synthase (NOS), or aminoguanidine, a competitive inhibitor of inducible nitric oxide synthase (iNOS), after 5 weeks of partial bladder outlet obstruction (BOO), in order to evaluate the role of constitutive and non-constitutive NOS in the pathogenesis of this experimental condition. Materials and Methods C57BL6 male mice were partially obstructed and randomly allocated into 6 groups: Sham, Sham + L-NAME, Sham + aminoguanidine, BOO, BOO + L-NAME and BOO + aminoguanidine. After 5 weeks, bladder weight was obtained and cystometry and tissue bath contractile studies were performed. Results BOO animals showed increase of non-voiding contractions (NVC) and bladder capacity, and also less contractile response to Carbachol and Electric Field Stimulation. Inhibition of NOS isoforms improved bladder capacity and compliance in BOO animals. L-NAME caused more NVC, prevented bladder weight gain and leaded to augmented contractile responses at muscarinic and electric stimulation. Aminoguanidine diminished NVC, but did not avoid bladder weight gain in BOO animals and did not improve contractile responses. Conclusion It can be hypothesized that chronic inhibition of three NOS isoforms in BOO animals leaded to worsening of bladder function, while selective inhibition of iNOS did not improve responses, what suggests that, in BOO animals, alterations are related to constitutive NOS.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382017000200356&lng=en&tlng=enNG-Nitroarginine Methyl EsterNitric OxideUrinary BladderUreteral Obstruction |
spellingShingle | Marcy Lancia Pereira Carlos Arturo Levi D’ancona Julio Alejandro Rojas-Moscoso Antonio Celso Saragossa Ramos Filho Fabiola Zakia Mónica Edson Antunes Effects of nitric oxide inhibitors in mice with bladder outlet obstruction International Brazilian Journal of Urology NG-Nitroarginine Methyl Ester Nitric Oxide Urinary Bladder Ureteral Obstruction |
title | Effects of nitric oxide inhibitors in mice with bladder outlet obstruction |
title_full | Effects of nitric oxide inhibitors in mice with bladder outlet obstruction |
title_fullStr | Effects of nitric oxide inhibitors in mice with bladder outlet obstruction |
title_full_unstemmed | Effects of nitric oxide inhibitors in mice with bladder outlet obstruction |
title_short | Effects of nitric oxide inhibitors in mice with bladder outlet obstruction |
title_sort | effects of nitric oxide inhibitors in mice with bladder outlet obstruction |
topic | NG-Nitroarginine Methyl Ester Nitric Oxide Urinary Bladder Ureteral Obstruction |
url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382017000200356&lng=en&tlng=en |
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