Nobiletin acts as a potential anticancer agent against osteosarcoma by regulating ERK and AKT signaling pathways
The current study was aimed to evaluate the anti-proliferative activity of nobiletin, a flavonoid, on osteosarcoma cell line, SaOS-2. Furthermore, we attempted to investigate the signaling pathways involved in the cell growth inhibition mechanism. Upon exposure, nobiletin showed significant cell inh...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Bangladesh Pharmacological Society
2014-09-01
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| Series: | Bangladesh Journal of Pharmacology |
| Subjects: | |
| Online Access: | https://www.banglajol.info/index.php/BJP/article/view/19567 |
| _version_ | 1811266462727274496 |
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| author | Fu-Wen Niu Yong-Jie Zhang Kun Li Mao-Shu Zhang |
| author_facet | Fu-Wen Niu Yong-Jie Zhang Kun Li Mao-Shu Zhang |
| author_sort | Fu-Wen Niu |
| collection | DOAJ |
| description | The current study was aimed to evaluate the anti-proliferative activity of nobiletin, a flavonoid, on osteosarcoma cell line, SaOS-2. Furthermore, we attempted to investigate the signaling pathways involved in the cell growth inhibition mechanism. Upon exposure, nobiletin showed significant cell inhibitory effect on SaOS-2 cells in a dose dependent manner as evidenced by MTT assay and LDH assay. Moreover, flow cytometric analysis demonstrated that upon treatment with nobiletin, SaOS-2 cells underwent apoptosis and showed increased number of cells arrested in G2/M phase. Activation of caspase-9 and -3 indicate the activation of apoptotic signaling pathway in SaOS-2 cells. Additionally, it was observed that the cell inhibitory effect was regulated by the activation of ERK signaling pathway while AKT signaling pathway was down regulated in SaOS-2 cells after 24 h of Nobiletin treatment. |
| first_indexed | 2024-04-12T20:43:42Z |
| format | Article |
| id | doaj.art-4a33386b6dad4bc68a65259fff5ef374 |
| institution | Directory Open Access Journal |
| issn | 1991-0088 |
| language | English |
| last_indexed | 2024-04-12T20:43:42Z |
| publishDate | 2014-09-01 |
| publisher | Bangladesh Pharmacological Society |
| record_format | Article |
| series | Bangladesh Journal of Pharmacology |
| spelling | doaj.art-4a33386b6dad4bc68a65259fff5ef3742022-12-22T03:17:21ZengBangladesh Pharmacological SocietyBangladesh Journal of Pharmacology1991-00882014-09-0193Nobiletin acts as a potential anticancer agent against osteosarcoma by regulating ERK and AKT signaling pathwaysFu-Wen Niu0Yong-Jie Zhang1Kun Li2Mao-Shu Zhang3Department of Orthopedics, Jining No.1 People’s Hospital in Shandong ProvinceDepartment of Orthopedics, JinXiang People’s Hospital in Shandong ProvinceDepartment of Gynecology and Obstetrics, Affiliated Hospital of Shandong Academy of Medical SciencesDepartment of Orthopedics, Jining No.1 People’s Hospital in Shandong ProvinceThe current study was aimed to evaluate the anti-proliferative activity of nobiletin, a flavonoid, on osteosarcoma cell line, SaOS-2. Furthermore, we attempted to investigate the signaling pathways involved in the cell growth inhibition mechanism. Upon exposure, nobiletin showed significant cell inhibitory effect on SaOS-2 cells in a dose dependent manner as evidenced by MTT assay and LDH assay. Moreover, flow cytometric analysis demonstrated that upon treatment with nobiletin, SaOS-2 cells underwent apoptosis and showed increased number of cells arrested in G2/M phase. Activation of caspase-9 and -3 indicate the activation of apoptotic signaling pathway in SaOS-2 cells. Additionally, it was observed that the cell inhibitory effect was regulated by the activation of ERK signaling pathway while AKT signaling pathway was down regulated in SaOS-2 cells after 24 h of Nobiletin treatment.https://www.banglajol.info/index.php/BJP/article/view/19567AntiproliferativeNobiletinOsteosarcomaSignaling pathway |
| spellingShingle | Fu-Wen Niu Yong-Jie Zhang Kun Li Mao-Shu Zhang Nobiletin acts as a potential anticancer agent against osteosarcoma by regulating ERK and AKT signaling pathways Bangladesh Journal of Pharmacology Antiproliferative Nobiletin Osteosarcoma Signaling pathway |
| title | Nobiletin acts as a potential anticancer agent against osteosarcoma by regulating ERK and AKT signaling pathways |
| title_full | Nobiletin acts as a potential anticancer agent against osteosarcoma by regulating ERK and AKT signaling pathways |
| title_fullStr | Nobiletin acts as a potential anticancer agent against osteosarcoma by regulating ERK and AKT signaling pathways |
| title_full_unstemmed | Nobiletin acts as a potential anticancer agent against osteosarcoma by regulating ERK and AKT signaling pathways |
| title_short | Nobiletin acts as a potential anticancer agent against osteosarcoma by regulating ERK and AKT signaling pathways |
| title_sort | nobiletin acts as a potential anticancer agent against osteosarcoma by regulating erk and akt signaling pathways |
| topic | Antiproliferative Nobiletin Osteosarcoma Signaling pathway |
| url | https://www.banglajol.info/index.php/BJP/article/view/19567 |
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