Upregulation of Nm23, a Metastasis Suppressor Gene, in Human Prostate Adenocarcinoma (PC-3) Cell Line Treated with Imatinib
Background: Prostate cancer is one of the most common cancers diagnosed in developed countries. Many studies have confirmed that the nm23 gene suppresses metastasis in different types of cancers. The effects of, imatinib as the first member of tyrosine kinases inhibitors, were showed in research and...
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Isfahan University of Medical Sciences
2015-01-01
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| Series: | مجله دانشکده پزشکی اصفهان |
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| Online Access: | http://jims.mui.ac.ir/index.php/jims/article/view/4602 |
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| author | Seyed Ataollah Sadat-Shandiz Mehdi Shafiee-Ardestani Shiva Irani Delavar Shahbazzadeh |
| author_facet | Seyed Ataollah Sadat-Shandiz Mehdi Shafiee-Ardestani Shiva Irani Delavar Shahbazzadeh |
| author_sort | Seyed Ataollah Sadat-Shandiz |
| collection | DOAJ |
| description | Background: Prostate cancer is one of the most common cancers diagnosed in developed countries. Many studies have confirmed that the nm23 gene suppresses metastasis in different types of cancers. The effects of, imatinib as the first member of tyrosine kinases inhibitors, were showed in research and treatment of solid tumors. The aim of the current study was to investigate the effect of imatinib on cell viability and suppressor metastasis nm23 gene expression in prostate cancer cell line.
Methods: In this study, prostate cancer (PC-3) human cell line was treated with various concentrations of imatinib for 48 hours. Cell viability was assessed using MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and the half maximal inhibitory concentration (IC50) value was determined. We extracted RNA molecules via using RNX solution, after which cDNA was synthesized. The precise primers for the nm23 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were designed via specific software. Then, the quantity of nm23 compared to GAPDH gene (reference gene) was analyzed using real-time polymerase chain reaction (PCR) method.
Findings: Imatinib exerted an inhibitory effect on the viability of metastatic PC-3 cells. The calculated nm23/GAPDH gene expression ratio was 1.62 ± 0.02 (P < 0.01) in 21/33 mM concentration of imatinib at 48 hours.
Conclusion: The results of this study showed that imatinib can inhibit metastasis via upregulating nm23 gene expression in prostate cancer adenocarcinoma PC-3 cell line. |
| first_indexed | 2024-03-12T10:29:36Z |
| format | Article |
| id | doaj.art-4a3488bb27374c0387fd814875b0fcf7 |
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| issn | 1027-7595 1735-854X |
| language | fas |
| last_indexed | 2024-03-12T10:29:36Z |
| publishDate | 2015-01-01 |
| publisher | Isfahan University of Medical Sciences |
| record_format | Article |
| series | مجله دانشکده پزشکی اصفهان |
| spelling | doaj.art-4a3488bb27374c0387fd814875b0fcf72023-09-02T09:20:36ZfasIsfahan University of Medical Sciencesمجله دانشکده پزشکی اصفهان1027-75951735-854X2015-01-0132309190719171734Upregulation of Nm23, a Metastasis Suppressor Gene, in Human Prostate Adenocarcinoma (PC-3) Cell Line Treated with ImatinibSeyed Ataollah Sadat-Shandiz0Mehdi Shafiee-Ardestani1Shiva Irani2Delavar Shahbazzadeh3PhD Student, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.Assistant Professor, Department of Radiopharmacy, School of Pharmacy, Tehran University of Medical Sciences, Tehran, IranAssistant Professor, Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, IranAssociate Professor, Biotechnology Research Center, Pasteur Institute of Iran AND Medical Biotechnology Group, Venom and Toxin Lab, Tehran, IranBackground: Prostate cancer is one of the most common cancers diagnosed in developed countries. Many studies have confirmed that the nm23 gene suppresses metastasis in different types of cancers. The effects of, imatinib as the first member of tyrosine kinases inhibitors, were showed in research and treatment of solid tumors. The aim of the current study was to investigate the effect of imatinib on cell viability and suppressor metastasis nm23 gene expression in prostate cancer cell line. Methods: In this study, prostate cancer (PC-3) human cell line was treated with various concentrations of imatinib for 48 hours. Cell viability was assessed using MTT assay [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] and the half maximal inhibitory concentration (IC50) value was determined. We extracted RNA molecules via using RNX solution, after which cDNA was synthesized. The precise primers for the nm23 and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) genes were designed via specific software. Then, the quantity of nm23 compared to GAPDH gene (reference gene) was analyzed using real-time polymerase chain reaction (PCR) method. Findings: Imatinib exerted an inhibitory effect on the viability of metastatic PC-3 cells. The calculated nm23/GAPDH gene expression ratio was 1.62 ± 0.02 (P < 0.01) in 21/33 mM concentration of imatinib at 48 hours. Conclusion: The results of this study showed that imatinib can inhibit metastasis via upregulating nm23 gene expression in prostate cancer adenocarcinoma PC-3 cell line.http://jims.mui.ac.ir/index.php/jims/article/view/4602Imatinibnm23Prostate cancerMetastasis |
| spellingShingle | Seyed Ataollah Sadat-Shandiz Mehdi Shafiee-Ardestani Shiva Irani Delavar Shahbazzadeh Upregulation of Nm23, a Metastasis Suppressor Gene, in Human Prostate Adenocarcinoma (PC-3) Cell Line Treated with Imatinib مجله دانشکده پزشکی اصفهان Imatinib nm23 Prostate cancer Metastasis |
| title | Upregulation of Nm23, a Metastasis Suppressor Gene, in Human Prostate Adenocarcinoma (PC-3) Cell Line Treated with Imatinib |
| title_full | Upregulation of Nm23, a Metastasis Suppressor Gene, in Human Prostate Adenocarcinoma (PC-3) Cell Line Treated with Imatinib |
| title_fullStr | Upregulation of Nm23, a Metastasis Suppressor Gene, in Human Prostate Adenocarcinoma (PC-3) Cell Line Treated with Imatinib |
| title_full_unstemmed | Upregulation of Nm23, a Metastasis Suppressor Gene, in Human Prostate Adenocarcinoma (PC-3) Cell Line Treated with Imatinib |
| title_short | Upregulation of Nm23, a Metastasis Suppressor Gene, in Human Prostate Adenocarcinoma (PC-3) Cell Line Treated with Imatinib |
| title_sort | upregulation of nm23 a metastasis suppressor gene in human prostate adenocarcinoma pc 3 cell line treated with imatinib |
| topic | Imatinib nm23 Prostate cancer Metastasis |
| url | http://jims.mui.ac.ir/index.php/jims/article/view/4602 |
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