Genetic characterization of Tunisian donkey with DNA microsatellites

The genetic characterization of Tunisian donkeys with microsatellite markers led to the identification of a total of 214 alleles, with an average of 14.27 alleles per locus. The number of effective alleles per locus (Ne) indicates a rich genetic polymorphism for the used markers. The number of allel...

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Main Authors: M AROUA, B JEMMALI, H OULED AHMED, S KHALDI, S BEN SAID, R BACCOUCHE, M MAHOUACHI
Format: Article
Language:English
Published: Indian Council of Agricultural Research 2019-09-01
Series:Indian Journal of Animal Sciences
Subjects:
Online Access:https://epubs.icar.org.in/index.php/IJAnS/article/view/93776
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author M AROUA
B JEMMALI
H OULED AHMED
S KHALDI
S BEN SAID
R BACCOUCHE
M MAHOUACHI
author_facet M AROUA
B JEMMALI
H OULED AHMED
S KHALDI
S BEN SAID
R BACCOUCHE
M MAHOUACHI
author_sort M AROUA
collection DOAJ
description The genetic characterization of Tunisian donkeys with microsatellite markers led to the identification of a total of 214 alleles, with an average of 14.27 alleles per locus. The number of effective alleles per locus (Ne) indicates a rich genetic polymorphism for the used markers. The number of alleles per locus (Na) is higher than Ne,confirming the high genetic diversity in the investigated population. Observed heterozygosis(Ho) for all the studied loci inthe entire population equals 0.744. The FIS index was significantly different from zero, pointing toan excess of heterozygotes. The mean of the number of effective migrants exchanged per generation (Nm) was 3.05 indicating a high level of gene exchange. Genetic distances and the principal coordinate analysis showed three distinct population groups/breeds, with a commongenetic structure for all the individuals. This genetic characterization of the Tunisian donkey population constitutes a valuable basis for further investigations, and to the elaboration of adequate conservation strategies.
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spelling doaj.art-4a34f556ae584933a296141bf19c3e6e2023-02-23T10:31:41ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272019-09-0189910.56093/ijans.v89i9.93776Genetic characterization of Tunisian donkey with DNA microsatellitesM AROUA0B JEMMALI1H OULED AHMED2S KHALDI3S BEN SAID4R BACCOUCHE5M MAHOUACHI6PhD Scholar, Carthage University, Carthage 1054, TunisiaAssociate Professor, Carthage University, Carthage 1054, TunisiaResearcher, Carthage University, Carthage 1054, TunisiaResearcher, Carthage University, Carthage 1054, TunisiaAssociate Professor, Carthage University, Carthage 1054, TunisiaPhD Scholar, Carthage University, Carthage 1054, TunisiaDirector, Carthage University, Carthage 1054, TunisiaThe genetic characterization of Tunisian donkeys with microsatellite markers led to the identification of a total of 214 alleles, with an average of 14.27 alleles per locus. The number of effective alleles per locus (Ne) indicates a rich genetic polymorphism for the used markers. The number of alleles per locus (Na) is higher than Ne,confirming the high genetic diversity in the investigated population. Observed heterozygosis(Ho) for all the studied loci inthe entire population equals 0.744. The FIS index was significantly different from zero, pointing toan excess of heterozygotes. The mean of the number of effective migrants exchanged per generation (Nm) was 3.05 indicating a high level of gene exchange. Genetic distances and the principal coordinate analysis showed three distinct population groups/breeds, with a commongenetic structure for all the individuals. This genetic characterization of the Tunisian donkey population constitutes a valuable basis for further investigations, and to the elaboration of adequate conservation strategies.https://epubs.icar.org.in/index.php/IJAnS/article/view/93776DiversityDonkeyGeneticMicrosatellitesTunisia
spellingShingle M AROUA
B JEMMALI
H OULED AHMED
S KHALDI
S BEN SAID
R BACCOUCHE
M MAHOUACHI
Genetic characterization of Tunisian donkey with DNA microsatellites
Indian Journal of Animal Sciences
Diversity
Donkey
Genetic
Microsatellites
Tunisia
title Genetic characterization of Tunisian donkey with DNA microsatellites
title_full Genetic characterization of Tunisian donkey with DNA microsatellites
title_fullStr Genetic characterization of Tunisian donkey with DNA microsatellites
title_full_unstemmed Genetic characterization of Tunisian donkey with DNA microsatellites
title_short Genetic characterization of Tunisian donkey with DNA microsatellites
title_sort genetic characterization of tunisian donkey with dna microsatellites
topic Diversity
Donkey
Genetic
Microsatellites
Tunisia
url https://epubs.icar.org.in/index.php/IJAnS/article/view/93776
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AT sbensaid geneticcharacterizationoftunisiandonkeywithdnamicrosatellites
AT rbaccouche geneticcharacterizationoftunisiandonkeywithdnamicrosatellites
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