Humic acid production from the degradation of Yima coal by Cunninghamella elegans combined with Bacillus sp.
Biodegradation is one of the important ways for the clean and efficient utilization of coal. However, the effectiveness of degradation by the combination of fungi and bacteria has not been well understood. In the present study, the combined degradation of the Yima coal was tested. The coal samples w...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | zho |
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Editorial Office of Journal of China Coal Society
2023-10-01
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| Series: | Meitan xuebao |
| Subjects: | |
| Online Access: | http://www.mtxb.com.cn/article/doi/10.13225/j.cnki.jccs.2022.1581 |
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| author | Linyong CHEN Jianmin LIU Yuxiang CUI Hengxing REN Han ZHAO Na ZHAO Yanli SONG Jiadong GUAN Jianglu NIU Guofu LI Baoyu WANG Huan HE |
| author_facet | Linyong CHEN Jianmin LIU Yuxiang CUI Hengxing REN Han ZHAO Na ZHAO Yanli SONG Jiadong GUAN Jianglu NIU Guofu LI Baoyu WANG Huan HE |
| author_sort | Linyong CHEN |
| collection | DOAJ |
| description | Biodegradation is one of the important ways for the clean and efficient utilization of coal. However, the effectiveness of degradation by the combination of fungi and bacteria has not been well understood. In the present study, the combined degradation of the Yima coal was tested. The coal samples were firstly oxidized with nitric acid, followed by cultured in the media of Cunninghamella elegans and Bacillus sp.. The absorbance of A450, pH and metallic element (Cr, As, Mn, Pb, Co, Ni, Cu, Zn, Mo) contents of the degradation solution were determined by UV-visible spectrophotometry, pH meter and inductively coupled plasma mass spectrometry, respectively. The humic acid was analyzed by element analyzer, Fourier transform infrared spectroscopy and gas chromatog-raphy-mass spectrometry. The results showed that the humic acid yields of C. elegans, Bacillus sp. and their mixture were 58.17%, 61.00% and 67.17%, respectively. The pH of the degradation solution of mixed strains was similar to that of the bacteria. The characteristic products of the bacteria degradation were detected in the humic acid samples derived from mixed strains, while the opposite was true for the fungi. It was suggested that the combination of the two strains enhanced the alkaline environment and improved the degradation rate of nitric acid-treated coal. The bacteria played a leading role in the degradation process. Metallic elements (Cr, As, Mn, Pb, Co, Ni, Cu, Zn, Mo) were transferred from coal to the degradation solution during the degradation process, and the contents of Cr, As, Pb, Ni, Cu and Mo were fitted with A450, the coefficient of determination (R2) were greater than 0.6. It indicated that the contents of these six metal elements in the degradation solution could represent the degradation rate. Chemically extracted humic acid and biologically extracted humic acid were rich in the active functional groups such as carboxyl, hydroxyl, carbonyl, long-chain fatty acids (C16, C18) and four pyrrole derivatives. The biologically extracted humic acid also contained fatty acids (C3, C4, C5, C13, C14, C15), of smaller molecular weight, as well as nitrogen-containing compounds such as two pyrrole derivatives and a furan. The contents of C and H elements in the biologically extracted humic acid were higher than that in the chemically extracted humic acid. |
| first_indexed | 2024-03-09T01:22:23Z |
| format | Article |
| id | doaj.art-4a35077a905c45db9c4c1d91a702a010 |
| institution | Directory Open Access Journal |
| issn | 0253-9993 |
| language | zho |
| last_indexed | 2024-03-09T01:22:23Z |
| publishDate | 2023-10-01 |
| publisher | Editorial Office of Journal of China Coal Society |
| record_format | Article |
| series | Meitan xuebao |
| spelling | doaj.art-4a35077a905c45db9c4c1d91a702a0102023-12-10T09:33:28ZzhoEditorial Office of Journal of China Coal SocietyMeitan xuebao0253-99932023-10-0148114224423210.13225/j.cnki.jccs.2022.15812022-1581Humic acid production from the degradation of Yima coal by Cunninghamella elegans combined with Bacillus sp.Linyong CHEN0Jianmin LIU1Yuxiang CUI2Hengxing REN3Han ZHAO4Na ZHAO5Yanli SONG6Jiadong GUAN7Jianglu NIU8Guofu LI9Baoyu WANG10Huan HE11Institute of Resources and Environment, Henan Polytechnic University, Jiaozuo 454000, ChinaInstitute of Resources and Environment, Henan Polytechnic University, Jiaozuo 454000, ChinaState Key Laboratory of Coal and CBM Co-mining, Jincheng 048000, ChinaInstitute of Resources and Environment, Henan Polytechnic University, Jiaozuo 454000, ChinaState Key Laboratory of Coal and CBM Co-mining, Jincheng 048000, ChinaState Key Laboratory of Coal and CBM Co-mining, Jincheng 048000, ChinaState Key Laboratory of Coal and CBM Co-mining, Jincheng 048000, ChinaState Key Laboratory of Coal and CBM Co-mining, Jincheng 048000, ChinaState Key Laboratory of Coal and CBM Co-mining, Jincheng 048000, ChinaInstitute of Resources and Environment, Henan Polytechnic University, Jiaozuo 454000, ChinaInstitute of Resources and Environment, Henan Polytechnic University, Jiaozuo 454000, ChinaSchool of Chemical Engineering, China University of Mining and Technology, Xuzhou 221116, ChinaBiodegradation is one of the important ways for the clean and efficient utilization of coal. However, the effectiveness of degradation by the combination of fungi and bacteria has not been well understood. In the present study, the combined degradation of the Yima coal was tested. The coal samples were firstly oxidized with nitric acid, followed by cultured in the media of Cunninghamella elegans and Bacillus sp.. The absorbance of A450, pH and metallic element (Cr, As, Mn, Pb, Co, Ni, Cu, Zn, Mo) contents of the degradation solution were determined by UV-visible spectrophotometry, pH meter and inductively coupled plasma mass spectrometry, respectively. The humic acid was analyzed by element analyzer, Fourier transform infrared spectroscopy and gas chromatog-raphy-mass spectrometry. The results showed that the humic acid yields of C. elegans, Bacillus sp. and their mixture were 58.17%, 61.00% and 67.17%, respectively. The pH of the degradation solution of mixed strains was similar to that of the bacteria. The characteristic products of the bacteria degradation were detected in the humic acid samples derived from mixed strains, while the opposite was true for the fungi. It was suggested that the combination of the two strains enhanced the alkaline environment and improved the degradation rate of nitric acid-treated coal. The bacteria played a leading role in the degradation process. Metallic elements (Cr, As, Mn, Pb, Co, Ni, Cu, Zn, Mo) were transferred from coal to the degradation solution during the degradation process, and the contents of Cr, As, Pb, Ni, Cu and Mo were fitted with A450, the coefficient of determination (R2) were greater than 0.6. It indicated that the contents of these six metal elements in the degradation solution could represent the degradation rate. Chemically extracted humic acid and biologically extracted humic acid were rich in the active functional groups such as carboxyl, hydroxyl, carbonyl, long-chain fatty acids (C16, C18) and four pyrrole derivatives. The biologically extracted humic acid also contained fatty acids (C3, C4, C5, C13, C14, C15), of smaller molecular weight, as well as nitrogen-containing compounds such as two pyrrole derivatives and a furan. The contents of C and H elements in the biologically extracted humic acid were higher than that in the chemically extracted humic acid.http://www.mtxb.com.cn/article/doi/10.13225/j.cnki.jccs.2022.1581cunninghamella elegansbacillus sp.biodegradation of coalmetallic elementsyima coal |
| spellingShingle | Linyong CHEN Jianmin LIU Yuxiang CUI Hengxing REN Han ZHAO Na ZHAO Yanli SONG Jiadong GUAN Jianglu NIU Guofu LI Baoyu WANG Huan HE Humic acid production from the degradation of Yima coal by Cunninghamella elegans combined with Bacillus sp. Meitan xuebao cunninghamella elegans bacillus sp. biodegradation of coal metallic elements yima coal |
| title | Humic acid production from the degradation of Yima coal by Cunninghamella elegans combined with Bacillus sp. |
| title_full | Humic acid production from the degradation of Yima coal by Cunninghamella elegans combined with Bacillus sp. |
| title_fullStr | Humic acid production from the degradation of Yima coal by Cunninghamella elegans combined with Bacillus sp. |
| title_full_unstemmed | Humic acid production from the degradation of Yima coal by Cunninghamella elegans combined with Bacillus sp. |
| title_short | Humic acid production from the degradation of Yima coal by Cunninghamella elegans combined with Bacillus sp. |
| title_sort | humic acid production from the degradation of yima coal by cunninghamella elegans combined with bacillus sp |
| topic | cunninghamella elegans bacillus sp. biodegradation of coal metallic elements yima coal |
| url | http://www.mtxb.com.cn/article/doi/10.13225/j.cnki.jccs.2022.1581 |
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