Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran
Background: Acanthamoeba spp. are free-living amoebae commonly found in the environmental sources such as water, soil, and air. This ubiquitous amoeba is the causative agent of amoebic kerati- tis (AK). The objective of the present study was to investigate the presence of Acanthamoeba spp. in water...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2015-10-01
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| Series: | Iranian Journal of Parasitology |
| Subjects: | |
| Online Access: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/280 |
| _version_ | 1830228973914685440 |
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| author | M. Rahdar M. Niyyati M. Salehi M. Feghhi M. Makvandi M. Pourmehdi S. Farnia |
| author_facet | M. Rahdar M. Niyyati M. Salehi M. Feghhi M. Makvandi M. Pourmehdi S. Farnia |
| author_sort | M. Rahdar |
| collection | DOAJ |
| description | Background: Acanthamoeba spp. are free-living amoebae commonly found in the environmental sources such as water, soil, and air. This ubiquitous amoeba is the causative agent of amoebic kerati- tis (AK). The objective of the present study was to investigate the presence of Acanthamoeba spp. in water and soil sources in Ahvaz City, Khuzestan Province, southern Iran.
Methods: In general, 110 samples of water and soil were taken from different localities of Ahvaz including agricultural canals, rivers, and swimming pools. Filtration and cultivation were carried out on non-nutrient agar medium (NNA). Axenic cultivation was performed for all of positive isolates. PCR analysis was conducted on positive samples. Sequencing was done for 15 PCR products. Geno - types were identified by Blast search and homology analysis.
Result: Acanthamoeba spp. was found in 43 (71.6%) of samples of water and 13 (26%) soil samples. Genotyping of 15 samples proved that Acanthamoeba belonged to T4 (86.6%), T2 (6.6%), and T5 (6.6%) genotypes.
Conclusion: TYI-S-33 medium could be better than PYG medium for Acanthamoeba axenic culture. |
| first_indexed | 2024-12-18T10:24:23Z |
| format | Article |
| id | doaj.art-4a39356a4ef645648462ca40727b0912 |
| institution | Directory Open Access Journal |
| issn | 1735-7020 2008-238X |
| language | English |
| last_indexed | 2024-12-18T10:24:23Z |
| publishDate | 2015-10-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Parasitology |
| spelling | doaj.art-4a39356a4ef645648462ca40727b09122022-12-21T21:11:01ZengTehran University of Medical SciencesIranian Journal of Parasitology1735-70202008-238X2015-10-0174Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern IranM. Rahdar0M. Niyyati1M. Salehi2M. Feghhi3M. Makvandi4M. Pourmehdi5S. Farnia6Cellular and Molecular Research Center, Tropical and Infectious Diseases Research Center & Department of Parasitology, School of Medicine, Ahvaz JundiShapur University of Medical Sciences, Ahvaz, Iran.Department of Parasitology and Mycology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran,Iran.Cellular and Molecular Research Center, Tropical and Infectious Diseases Research Center & Department of Parasitology, School of Medicine, Ahvaz JundiShapur University of Medical Sciences, Ahvaz, Iran.Department of Ophthalmology, Imam Khomeini Hospital, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.Department of Virology, School of Medicine, Ahvaz JundiShapur University of Medical Sciences, Ahvaz, Iran.Department of Epidemiology and Statistical, School of Veterinary, Shahid Chamran University, Ahvaz, Iran.Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran,Iran.Background: Acanthamoeba spp. are free-living amoebae commonly found in the environmental sources such as water, soil, and air. This ubiquitous amoeba is the causative agent of amoebic kerati- tis (AK). The objective of the present study was to investigate the presence of Acanthamoeba spp. in water and soil sources in Ahvaz City, Khuzestan Province, southern Iran. Methods: In general, 110 samples of water and soil were taken from different localities of Ahvaz including agricultural canals, rivers, and swimming pools. Filtration and cultivation were carried out on non-nutrient agar medium (NNA). Axenic cultivation was performed for all of positive isolates. PCR analysis was conducted on positive samples. Sequencing was done for 15 PCR products. Geno - types were identified by Blast search and homology analysis. Result: Acanthamoeba spp. was found in 43 (71.6%) of samples of water and 13 (26%) soil samples. Genotyping of 15 samples proved that Acanthamoeba belonged to T4 (86.6%), T2 (6.6%), and T5 (6.6%) genotypes. Conclusion: TYI-S-33 medium could be better than PYG medium for Acanthamoeba axenic culture.https://ijpa.tums.ac.ir/index.php/ijpa/article/view/280Acanthamoeba spp.WaterSoilTYI-S-33Iran |
| spellingShingle | M. Rahdar M. Niyyati M. Salehi M. Feghhi M. Makvandi M. Pourmehdi S. Farnia Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran Iranian Journal of Parasitology Acanthamoeba spp. Water Soil TYI-S-33 Iran |
| title | Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran |
| title_full | Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran |
| title_fullStr | Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran |
| title_full_unstemmed | Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran |
| title_short | Isolation and Genotyping of Acanthamoeba Strains from Environmental Sources in Ahvaz City, Khuzestan Province, Southern Iran |
| title_sort | isolation and genotyping of acanthamoeba strains from environmental sources in ahvaz city khuzestan province southern iran |
| topic | Acanthamoeba spp. Water Soil TYI-S-33 Iran |
| url | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/280 |
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