Impact of Myeloid p38α/MAPK on Orthodontic Tooth Movement
<i>Objectives</i>: Myeloid p38α/MAPK regulate and coordinate osteoclastogenesis. The present study was conducted to investigate the role of myeloid p38α/MAPK during orthodontic tooth movement. <i>Methods</i>: Orthodontic tooth movement was performed in wildtype and <i>p...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-03-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/11/7/1796 |
| _version_ | 1797438859759321088 |
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| author | Christian Kirschneck Hendrik Nusser Jonathan Jantsch Peter Proff Agnes Schröder |
| author_facet | Christian Kirschneck Hendrik Nusser Jonathan Jantsch Peter Proff Agnes Schröder |
| author_sort | Christian Kirschneck |
| collection | DOAJ |
| description | <i>Objectives</i>: Myeloid p38α/MAPK regulate and coordinate osteoclastogenesis. The present study was conducted to investigate the role of myeloid p38α/MAPK during orthodontic tooth movement. <i>Methods</i>: Orthodontic tooth movement was performed in wildtype and <i>p38α</i><sup>Δmyel</sup> mice lacking p38α/MAPK expression in myeloid cells. First, bone parameter as well as osteoblast and osteoclast number were determined in tibiae. RNA was isolated from the untreated and orthodontically treated maxillary jaw side and expression of genes involved in inflammation and bone remodelling were analysed. Finally, periodontal bone loss, alveolar bone density and extent of orthodontic tooth movement were assessed. <i>Results</i>: Bone density was increased in <i>p38α</i><sup>Δmyel</sup> mice compared to wildtype mice in tibiae (<i>p</i> = 0.043) and alveolar bone (<i>p</i> = 0.003). This was accompanied by a reduced osteoclast number in tibiae (<i>p</i> = 0.005) and TRAP5b in serum (<i>p</i> = 0.015). Accordingly, expression of osteoclast-specific genes was reduced in <i>p38α</i><sup>Δmyel</sup> mice. Extent of tooth movement was reduced in <i>p38α</i><sup>Δmyel</sup> mice (<i>p</i> = 0.024). This may be due to the higher bone density of the p38αΔmyel mice. <i>Conclusions</i>: Myeloid p38α/MAPK thus appears to play a regulatory role during orthodontic tooth movement by regulating osteoclastogenesis. |
| first_indexed | 2024-03-09T11:43:24Z |
| format | Article |
| id | doaj.art-4a394996376e4a9bb4eec6d89561e039 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-09T11:43:24Z |
| publishDate | 2022-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-4a394996376e4a9bb4eec6d89561e0392023-11-30T23:26:59ZengMDPI AGJournal of Clinical Medicine2077-03832022-03-01117179610.3390/jcm11071796Impact of Myeloid p38α/MAPK on Orthodontic Tooth MovementChristian Kirschneck0Hendrik Nusser1Jonathan Jantsch2Peter Proff3Agnes Schröder4Department of Orthodontics, University Hospital Regensburg, 93053 Regensburg, GermanyDepartment of Orthodontics, University Hospital Regensburg, 93053 Regensburg, GermanyInstitute of Clinical Microbiology and Hygiene, University Hospital Regensburg, 93053 Regensburg, GermanyDepartment of Orthodontics, University Hospital Regensburg, 93053 Regensburg, GermanyDepartment of Orthodontics, University Hospital Regensburg, 93053 Regensburg, Germany<i>Objectives</i>: Myeloid p38α/MAPK regulate and coordinate osteoclastogenesis. The present study was conducted to investigate the role of myeloid p38α/MAPK during orthodontic tooth movement. <i>Methods</i>: Orthodontic tooth movement was performed in wildtype and <i>p38α</i><sup>Δmyel</sup> mice lacking p38α/MAPK expression in myeloid cells. First, bone parameter as well as osteoblast and osteoclast number were determined in tibiae. RNA was isolated from the untreated and orthodontically treated maxillary jaw side and expression of genes involved in inflammation and bone remodelling were analysed. Finally, periodontal bone loss, alveolar bone density and extent of orthodontic tooth movement were assessed. <i>Results</i>: Bone density was increased in <i>p38α</i><sup>Δmyel</sup> mice compared to wildtype mice in tibiae (<i>p</i> = 0.043) and alveolar bone (<i>p</i> = 0.003). This was accompanied by a reduced osteoclast number in tibiae (<i>p</i> = 0.005) and TRAP5b in serum (<i>p</i> = 0.015). Accordingly, expression of osteoclast-specific genes was reduced in <i>p38α</i><sup>Δmyel</sup> mice. Extent of tooth movement was reduced in <i>p38α</i><sup>Δmyel</sup> mice (<i>p</i> = 0.024). This may be due to the higher bone density of the p38αΔmyel mice. <i>Conclusions</i>: Myeloid p38α/MAPK thus appears to play a regulatory role during orthodontic tooth movement by regulating osteoclastogenesis.https://www.mdpi.com/2077-0383/11/7/1796p38 mitogen-activated protein kinasesmyeloid cellsorthodontic tooth movement |
| spellingShingle | Christian Kirschneck Hendrik Nusser Jonathan Jantsch Peter Proff Agnes Schröder Impact of Myeloid p38α/MAPK on Orthodontic Tooth Movement Journal of Clinical Medicine p38 mitogen-activated protein kinases myeloid cells orthodontic tooth movement |
| title | Impact of Myeloid p38α/MAPK on Orthodontic Tooth Movement |
| title_full | Impact of Myeloid p38α/MAPK on Orthodontic Tooth Movement |
| title_fullStr | Impact of Myeloid p38α/MAPK on Orthodontic Tooth Movement |
| title_full_unstemmed | Impact of Myeloid p38α/MAPK on Orthodontic Tooth Movement |
| title_short | Impact of Myeloid p38α/MAPK on Orthodontic Tooth Movement |
| title_sort | impact of myeloid p38α mapk on orthodontic tooth movement |
| topic | p38 mitogen-activated protein kinases myeloid cells orthodontic tooth movement |
| url | https://www.mdpi.com/2077-0383/11/7/1796 |
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