Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves

Ultrasonic-assisted extraction of flavonoids from Camellia fascicularis leaves was optimized using response surface methodology. The optimal extracting conditions of flavonoids were determined to be the ratio of liquid to raw material of 60 mL/g, ethanol concentration of 40%, extraction temperature...

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Main Authors: Yun Liu, Xulu Luo, Zengquan Lan, Junrong Tang, Ping Zhao, Huan Kan
Format: Article
Language:English
Published: Taylor & Francis Group 2018-01-01
Series:CyTA - Journal of Food
Subjects:
Online Access:http://dx.doi.org/10.1080/19476337.2017.1343867
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author Yun Liu
Xulu Luo
Zengquan Lan
Junrong Tang
Ping Zhao
Huan Kan
author_facet Yun Liu
Xulu Luo
Zengquan Lan
Junrong Tang
Ping Zhao
Huan Kan
author_sort Yun Liu
collection DOAJ
description Ultrasonic-assisted extraction of flavonoids from Camellia fascicularis leaves was optimized using response surface methodology. The optimal extracting conditions of flavonoids were determined to be the ratio of liquid to raw material of 60 mL/g, ethanol concentration of 40%, extraction temperature of 72.3°C, and extraction time of 1.6 h, which contributed to the highest flavonoids yield of 4.765%. The crude flavonoids were purified through an AB-8 macroporous adsorption resin column, eluted with ethanol concentration of 40%, and purified flavonoids were obtained. In vitro antioxidant assay demonstrated that purified flavonoids showed significant antioxidant capacities in a concentration-dependent manner for scavenging hydroxyl radical, superoxide anion radical, 2,2-diphenyl-1-picrylhydrazyl radical, and azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical. Overall, ultrasonic-assisted extraction was found to be an effective method of extracting flavonoids from C. fascicularis leaves, which might be explored as potential natural antioxidant.
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spelling doaj.art-4a3a037199054937b2d2dcdd4be48f912022-12-22T02:09:13ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452018-01-0116110511210.1080/19476337.2017.13438671343867Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leavesYun Liu0Xulu Luo1Zengquan Lan2Junrong Tang3Ping Zhao4Huan Kan5Southwest Forestry UniversitySouthwest Forestry UniversitySouthwest Forestry UniversitySouthwest Forestry UniversitySouthwest Forestry UniversitySouthwest Forestry UniversityUltrasonic-assisted extraction of flavonoids from Camellia fascicularis leaves was optimized using response surface methodology. The optimal extracting conditions of flavonoids were determined to be the ratio of liquid to raw material of 60 mL/g, ethanol concentration of 40%, extraction temperature of 72.3°C, and extraction time of 1.6 h, which contributed to the highest flavonoids yield of 4.765%. The crude flavonoids were purified through an AB-8 macroporous adsorption resin column, eluted with ethanol concentration of 40%, and purified flavonoids were obtained. In vitro antioxidant assay demonstrated that purified flavonoids showed significant antioxidant capacities in a concentration-dependent manner for scavenging hydroxyl radical, superoxide anion radical, 2,2-diphenyl-1-picrylhydrazyl radical, and azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical. Overall, ultrasonic-assisted extraction was found to be an effective method of extracting flavonoids from C. fascicularis leaves, which might be explored as potential natural antioxidant.http://dx.doi.org/10.1080/19476337.2017.1343867Camellia fascicularis Hung T. Changflavonoidsultrasonic-assisted extractionRSMantioxidant capacity
spellingShingle Yun Liu
Xulu Luo
Zengquan Lan
Junrong Tang
Ping Zhao
Huan Kan
Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves
CyTA - Journal of Food
Camellia fascicularis Hung T. Chang
flavonoids
ultrasonic-assisted extraction
RSM
antioxidant capacity
title Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves
title_full Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves
title_fullStr Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves
title_full_unstemmed Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves
title_short Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves
title_sort ultrasonic assisted extraction and antioxidant capacities of flavonoids from camellia fascicularis leaves
topic Camellia fascicularis Hung T. Chang
flavonoids
ultrasonic-assisted extraction
RSM
antioxidant capacity
url http://dx.doi.org/10.1080/19476337.2017.1343867
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AT zengquanlan ultrasonicassistedextractionandantioxidantcapacitiesofflavonoidsfromcamelliafascicularisleaves
AT junrongtang ultrasonicassistedextractionandantioxidantcapacitiesofflavonoidsfromcamelliafascicularisleaves
AT pingzhao ultrasonicassistedextractionandantioxidantcapacitiesofflavonoidsfromcamelliafascicularisleaves
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