Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves
Ultrasonic-assisted extraction of flavonoids from Camellia fascicularis leaves was optimized using response surface methodology. The optimal extracting conditions of flavonoids were determined to be the ratio of liquid to raw material of 60 mL/g, ethanol concentration of 40%, extraction temperature...
Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
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Taylor & Francis Group
2018-01-01
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Series: | CyTA - Journal of Food |
Subjects: | |
Online Access: | http://dx.doi.org/10.1080/19476337.2017.1343867 |
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author | Yun Liu Xulu Luo Zengquan Lan Junrong Tang Ping Zhao Huan Kan |
author_facet | Yun Liu Xulu Luo Zengquan Lan Junrong Tang Ping Zhao Huan Kan |
author_sort | Yun Liu |
collection | DOAJ |
description | Ultrasonic-assisted extraction of flavonoids from Camellia fascicularis leaves was optimized using response surface methodology. The optimal extracting conditions of flavonoids were determined to be the ratio of liquid to raw material of 60 mL/g, ethanol concentration of 40%, extraction temperature of 72.3°C, and extraction time of 1.6 h, which contributed to the highest flavonoids yield of 4.765%. The crude flavonoids were purified through an AB-8 macroporous adsorption resin column, eluted with ethanol concentration of 40%, and purified flavonoids were obtained. In vitro antioxidant assay demonstrated that purified flavonoids showed significant antioxidant capacities in a concentration-dependent manner for scavenging hydroxyl radical, superoxide anion radical, 2,2-diphenyl-1-picrylhydrazyl radical, and azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical. Overall, ultrasonic-assisted extraction was found to be an effective method of extracting flavonoids from C. fascicularis leaves, which might be explored as potential natural antioxidant. |
first_indexed | 2024-04-14T05:48:25Z |
format | Article |
id | doaj.art-4a3a037199054937b2d2dcdd4be48f91 |
institution | Directory Open Access Journal |
issn | 1947-6337 1947-6345 |
language | English |
last_indexed | 2024-04-14T05:48:25Z |
publishDate | 2018-01-01 |
publisher | Taylor & Francis Group |
record_format | Article |
series | CyTA - Journal of Food |
spelling | doaj.art-4a3a037199054937b2d2dcdd4be48f912022-12-22T02:09:13ZengTaylor & Francis GroupCyTA - Journal of Food1947-63371947-63452018-01-0116110511210.1080/19476337.2017.13438671343867Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leavesYun Liu0Xulu Luo1Zengquan Lan2Junrong Tang3Ping Zhao4Huan Kan5Southwest Forestry UniversitySouthwest Forestry UniversitySouthwest Forestry UniversitySouthwest Forestry UniversitySouthwest Forestry UniversitySouthwest Forestry UniversityUltrasonic-assisted extraction of flavonoids from Camellia fascicularis leaves was optimized using response surface methodology. The optimal extracting conditions of flavonoids were determined to be the ratio of liquid to raw material of 60 mL/g, ethanol concentration of 40%, extraction temperature of 72.3°C, and extraction time of 1.6 h, which contributed to the highest flavonoids yield of 4.765%. The crude flavonoids were purified through an AB-8 macroporous adsorption resin column, eluted with ethanol concentration of 40%, and purified flavonoids were obtained. In vitro antioxidant assay demonstrated that purified flavonoids showed significant antioxidant capacities in a concentration-dependent manner for scavenging hydroxyl radical, superoxide anion radical, 2,2-diphenyl-1-picrylhydrazyl radical, and azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt radical. Overall, ultrasonic-assisted extraction was found to be an effective method of extracting flavonoids from C. fascicularis leaves, which might be explored as potential natural antioxidant.http://dx.doi.org/10.1080/19476337.2017.1343867Camellia fascicularis Hung T. Changflavonoidsultrasonic-assisted extractionRSMantioxidant capacity |
spellingShingle | Yun Liu Xulu Luo Zengquan Lan Junrong Tang Ping Zhao Huan Kan Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves CyTA - Journal of Food Camellia fascicularis Hung T. Chang flavonoids ultrasonic-assisted extraction RSM antioxidant capacity |
title | Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves |
title_full | Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves |
title_fullStr | Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves |
title_full_unstemmed | Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves |
title_short | Ultrasonic-assisted extraction and antioxidant capacities of flavonoids from Camellia fascicularis leaves |
title_sort | ultrasonic assisted extraction and antioxidant capacities of flavonoids from camellia fascicularis leaves |
topic | Camellia fascicularis Hung T. Chang flavonoids ultrasonic-assisted extraction RSM antioxidant capacity |
url | http://dx.doi.org/10.1080/19476337.2017.1343867 |
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