Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana
Abstract Background Klebsiella pneumoniae is one of the main pathogens of clinical isolation and nosocomial infections, as K. pneumoniae show broad-spectrum resistance to β-lactam and carbapenem antibiotics. It is emerging clinical need for a safe and effective drug to anti-K. pneumoniae. At present...
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | BMC Microbiology |
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| Online Access: | https://doi.org/10.1186/s12866-023-02909-7 |
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| author | Yan Ma Ping Guo Xueqin Chen Minhua Xu Wenbin Liu Xiaobao Jin |
| author_facet | Yan Ma Ping Guo Xueqin Chen Minhua Xu Wenbin Liu Xiaobao Jin |
| author_sort | Yan Ma |
| collection | DOAJ |
| description | Abstract Background Klebsiella pneumoniae is one of the main pathogens of clinical isolation and nosocomial infections, as K. pneumoniae show broad-spectrum resistance to β-lactam and carbapenem antibiotics. It is emerging clinical need for a safe and effective drug to anti-K. pneumoniae. At present, Achromobacter mainly focused on its degradation of petroleum hydrocarbons, polycyclic aromatic hydrocarbons, assisting insects to decompose, degrade heavy metals and utilize organic matter, but there were few reports on the antibacterial activity of the secondary metabolites of Achromobacter. Results In this study, a strain WA5-4-31 from the intestinal tract of Periplaneta americana exhibited strong activity against K. Pneumoniae through preliminary screening. The strain was determined to be Achromobacter sp. through the morphological characteristics, genotyping and phylogenetic tree analysis, which is homologous to Achromobacter ruhlandii by 99%, its accession numbe in GenBank at National Center for Biotechnology Information (NCBI) is MN007235, and its deposit number was GDMCC NO.1.2520. Six compounds (Actinomycin D, Actinomycin X2, Collismycin A, Citrinin, Neoechinulin A and Cytochalasin E) were isolated and determined by activity tracking, chemical separation, nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. Among them, Actinomycin D, Actinomycin X2, Collismycin A, Citrinin and Cytochalasin E showed a good effect on anti-K. pneumoniae, with MIC values of 16–64 µg/mL. Conclusions The study reported Achromobacter, which was from the intestinal tract of Periplaneta americana with the activity against K. Pneumoniae, can produce antibacterial compounds for the first time. It lays the foundation for development of secondary metabolites of insect intestinal microorganisms. |
| first_indexed | 2024-03-13T06:13:27Z |
| format | Article |
| id | doaj.art-4a3d9f29d76f4f0b8e77430456ad597d |
| institution | Directory Open Access Journal |
| issn | 1471-2180 |
| language | English |
| last_indexed | 2024-03-13T06:13:27Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Microbiology |
| spelling | doaj.art-4a3d9f29d76f4f0b8e77430456ad597d2023-06-11T11:07:03ZengBMCBMC Microbiology1471-21802023-06-012311910.1186/s12866-023-02909-7Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americanaYan Ma0Ping Guo1Xueqin Chen2Minhua Xu3Wenbin Liu4Xiaobao Jin5School of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversityGuangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical UniversitySchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversityGuangdong Provincial Key Laboratory of Pharmaceutical Bioactive Substances, Guangdong Pharmaceutical UniversitySchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversitySchool of Life Sciences and Biopharmaceutics, Guangdong Pharmaceutical UniversityAbstract Background Klebsiella pneumoniae is one of the main pathogens of clinical isolation and nosocomial infections, as K. pneumoniae show broad-spectrum resistance to β-lactam and carbapenem antibiotics. It is emerging clinical need for a safe and effective drug to anti-K. pneumoniae. At present, Achromobacter mainly focused on its degradation of petroleum hydrocarbons, polycyclic aromatic hydrocarbons, assisting insects to decompose, degrade heavy metals and utilize organic matter, but there were few reports on the antibacterial activity of the secondary metabolites of Achromobacter. Results In this study, a strain WA5-4-31 from the intestinal tract of Periplaneta americana exhibited strong activity against K. Pneumoniae through preliminary screening. The strain was determined to be Achromobacter sp. through the morphological characteristics, genotyping and phylogenetic tree analysis, which is homologous to Achromobacter ruhlandii by 99%, its accession numbe in GenBank at National Center for Biotechnology Information (NCBI) is MN007235, and its deposit number was GDMCC NO.1.2520. Six compounds (Actinomycin D, Actinomycin X2, Collismycin A, Citrinin, Neoechinulin A and Cytochalasin E) were isolated and determined by activity tracking, chemical separation, nuclear magnetic resonance (NMR) and mass spectrometry (MS) analysis. Among them, Actinomycin D, Actinomycin X2, Collismycin A, Citrinin and Cytochalasin E showed a good effect on anti-K. pneumoniae, with MIC values of 16–64 µg/mL. Conclusions The study reported Achromobacter, which was from the intestinal tract of Periplaneta americana with the activity against K. Pneumoniae, can produce antibacterial compounds for the first time. It lays the foundation for development of secondary metabolites of insect intestinal microorganisms.https://doi.org/10.1186/s12866-023-02909-7Klebsiella pneumoniaPeriplaneta americana intestineAchromobacterSecondary metabolitesAntibacterial activity |
| spellingShingle | Yan Ma Ping Guo Xueqin Chen Minhua Xu Wenbin Liu Xiaobao Jin Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana BMC Microbiology Klebsiella pneumonia Periplaneta americana intestine Achromobacter Secondary metabolites Antibacterial activity |
| title | Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana |
| title_full | Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana |
| title_fullStr | Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana |
| title_full_unstemmed | Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana |
| title_short | Anti-Klebsiella pneumoniae activity of secondary metabolism of Achromobacter from the intestine of Periplaneta americana |
| title_sort | anti klebsiella pneumoniae activity of secondary metabolism of achromobacter from the intestine of periplaneta americana |
| topic | Klebsiella pneumonia Periplaneta americana intestine Achromobacter Secondary metabolites Antibacterial activity |
| url | https://doi.org/10.1186/s12866-023-02909-7 |
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