MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRAS
Background/Aims: MicroRNAs (miRNAs) have emerged as major regulators of tumour development and progression in non-small cell lung cancer (NSCLC). However, the role of miR-193a-3p in NSCLC is still unclear. Methods: Quantitative RT-PCR was used to detect miR-193a-3p expression levels in NSCLC tumour...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2017-11-01
|
| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | https://www.karger.com/Article/FullText/485491 |
| _version_ | 1819291651980394496 |
|---|---|
| author | Qian Fan Xiuting Hu Haiyang Zhang Shengguang Wang Huilai Zhang Chaoying You Chen-Yu Zhang Hongwei Liang Xi Chen Yi Ba |
| author_facet | Qian Fan Xiuting Hu Haiyang Zhang Shengguang Wang Huilai Zhang Chaoying You Chen-Yu Zhang Hongwei Liang Xi Chen Yi Ba |
| author_sort | Qian Fan |
| collection | DOAJ |
| description | Background/Aims: MicroRNAs (miRNAs) have emerged as major regulators of tumour development and progression in non-small cell lung cancer (NSCLC). However, the role of miR-193a-3p in NSCLC is still unclear. Methods: Quantitative RT-PCR was used to detect miR-193a-3p expression levels in NSCLC tumour tissues. CCK8, EdU and cell migration assays were performed to analyse the biological functions of miR-193a-3p in NSCLC cells. Luciferase reporter assays were used to validate the bioinformatics-predicted target genes of miR-193a-3p. Western blotting and RNA/DNA interference carried out to evaluate the association between miR-193a-3p and KRAS. Results: miR-193a-3p expression was decreased in the NSCLC tumour tissues. We investigated the biological effects of miR-193a-3p both in vivo and in vitro and found that enforced expression of miR-193a-3p inhibited tumour formation and suppressed cell proliferation and cell migration. KRAS was found to be a potential target of miR-193a-3p, and dual luciferase reporter assays showed that miR-193a-3p directly binds to the 3’-untranslated region (3’-UTR) of KRAS mRNA. In addition, we found that changing the expression of KRAS had the opposite results to those induced by miR-193a-3p in the NSCLC cells. Importantly, simultaneous overexpression of miR-193a-3p and KRAS could counteract the effects of both on cellular functions. Conclusion: These findings highlight an important role for miR-193a-3p as a tumour suppressor in NSCLC pathogenesis via the regulation of KRAS expression. |
| first_indexed | 2024-12-24T03:42:02Z |
| format | Article |
| id | doaj.art-4a3dd15765954a81be62073d66b91530 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-24T03:42:02Z |
| publishDate | 2017-11-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-4a3dd15765954a81be62073d66b915302022-12-21T17:16:52ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782017-11-014441311132410.1159/000485491485491MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRASQian FanXiuting HuHaiyang ZhangShengguang WangHuilai ZhangChaoying YouChen-Yu ZhangHongwei LiangXi ChenYi BaBackground/Aims: MicroRNAs (miRNAs) have emerged as major regulators of tumour development and progression in non-small cell lung cancer (NSCLC). However, the role of miR-193a-3p in NSCLC is still unclear. Methods: Quantitative RT-PCR was used to detect miR-193a-3p expression levels in NSCLC tumour tissues. CCK8, EdU and cell migration assays were performed to analyse the biological functions of miR-193a-3p in NSCLC cells. Luciferase reporter assays were used to validate the bioinformatics-predicted target genes of miR-193a-3p. Western blotting and RNA/DNA interference carried out to evaluate the association between miR-193a-3p and KRAS. Results: miR-193a-3p expression was decreased in the NSCLC tumour tissues. We investigated the biological effects of miR-193a-3p both in vivo and in vitro and found that enforced expression of miR-193a-3p inhibited tumour formation and suppressed cell proliferation and cell migration. KRAS was found to be a potential target of miR-193a-3p, and dual luciferase reporter assays showed that miR-193a-3p directly binds to the 3’-untranslated region (3’-UTR) of KRAS mRNA. In addition, we found that changing the expression of KRAS had the opposite results to those induced by miR-193a-3p in the NSCLC cells. Importantly, simultaneous overexpression of miR-193a-3p and KRAS could counteract the effects of both on cellular functions. Conclusion: These findings highlight an important role for miR-193a-3p as a tumour suppressor in NSCLC pathogenesis via the regulation of KRAS expression.https://www.karger.com/Article/FullText/485491MicroRNAKRASNSCLCmiR-193a-3p |
| spellingShingle | Qian Fan Xiuting Hu Haiyang Zhang Shengguang Wang Huilai Zhang Chaoying You Chen-Yu Zhang Hongwei Liang Xi Chen Yi Ba MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRAS Cellular Physiology and Biochemistry MicroRNA KRAS NSCLC miR-193a-3p |
| title | MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRAS |
| title_full | MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRAS |
| title_fullStr | MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRAS |
| title_full_unstemmed | MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRAS |
| title_short | MiR-193a-3p is an Important Tumour Suppressor in Lung Cancer and Directly Targets KRAS |
| title_sort | mir 193a 3p is an important tumour suppressor in lung cancer and directly targets kras |
| topic | MicroRNA KRAS NSCLC miR-193a-3p |
| url | https://www.karger.com/Article/FullText/485491 |
| work_keys_str_mv | AT qianfan mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras AT xiutinghu mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras AT haiyangzhang mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras AT shengguangwang mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras AT huilaizhang mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras AT chaoyingyou mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras AT chenyuzhang mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras AT hongweiliang mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras AT xichen mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras AT yiba mir193a3pisanimportanttumoursuppressorinlungcanceranddirectlytargetskras |