Neuroprotective potential of Cannabis sativa-based oils in Caenorhabditis elegans
Abstract Substances from the Cannabis sativa species, especially cannabidiol (CBD) and Delta-9-tetrahydrocannabinol (Δ9-THC), have attracted medical attention in recent years. The actions of these two main cannabinoids modulate the cholinergic nervous system (CholNS) involving development, synaptic...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2022-09-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-19598-3 |
| _version_ | 1798036363271995392 |
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| author | Ana Paula Vanin Wagner Antonio Tamagno Carla Alves Letícia Mesacasa Luciani Figueiredo Santin Nathália Tafarel Sutorillo Denise Bilibio Caroline Müller Leandro Galon Rosilene Rodrigues Kaizer |
| author_facet | Ana Paula Vanin Wagner Antonio Tamagno Carla Alves Letícia Mesacasa Luciani Figueiredo Santin Nathália Tafarel Sutorillo Denise Bilibio Caroline Müller Leandro Galon Rosilene Rodrigues Kaizer |
| author_sort | Ana Paula Vanin |
| collection | DOAJ |
| description | Abstract Substances from the Cannabis sativa species, especially cannabidiol (CBD) and Delta-9-tetrahydrocannabinol (Δ9-THC), have attracted medical attention in recent years. The actions of these two main cannabinoids modulate the cholinergic nervous system (CholNS) involving development, synaptic plasticity, and response to endogenous and environmental damage, as a characteristic of many neurodegenerative diseases. The dynamics of these diseases are mediated by specific neurotransmitters, such as the GABAergic nervous system (GNS) and the CholNS. The nematode Caenorhabditis elegans is an important experimental model, which has different neurotransmitter systems that coordinate its behavior and has a transgene strain that encodes the human β-amyloid 1–42 peptide in body wall muscle, one of the main proteins involved in Alzheimer´s disease. Therefore, the objective of this study was to evaluate the protective potential of terpenoids found in C. sativa in the GNS and CholNS of C. elegans. The effect of two C. sativa oils with variations in CBD and THC concentrations on acetylcholinesterase (AChE) activity, lipid peroxidation, and behavior of C. elegans was evaluated. C. sativa oils were efficient in increasing pharyngeal pumping rate and reducing defecation cycle, AChE activity, and ROS levels in N2 strains. In the muscle:Abeta1-42 strain, mainly when using CBD oil, worm movement, body bends, and pharyngeal pumping were increased, with a reduced AChE activity. Consequently, greater investments in scientific research are needed, in addition to breaking the taboo on the use of the C. sativa plant as an alternative for medicinal use, especially in neurodegenerative diseases, which have already shown positive initial results. |
| first_indexed | 2024-04-11T21:11:49Z |
| format | Article |
| id | doaj.art-4a3df63dd9764b19a9e824940831a19d |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-11T21:11:49Z |
| publishDate | 2022-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-4a3df63dd9764b19a9e824940831a19d2022-12-22T04:03:00ZengNature PortfolioScientific Reports2045-23222022-09-0112111210.1038/s41598-022-19598-3Neuroprotective potential of Cannabis sativa-based oils in Caenorhabditis elegansAna Paula Vanin0Wagner Antonio Tamagno1Carla Alves2Letícia Mesacasa3Luciani Figueiredo Santin4Nathália Tafarel Sutorillo5Denise Bilibio6Caroline Müller7Leandro Galon8Rosilene Rodrigues Kaizer9Universidade Federal da Fronteira SulUniversidade Federal de Santa MariaInstituto Federal de Educação, Ciência e Tecnologia do Rio Grande do SulUniversidade de Santa Cruz do SulInstituto Federal de Educação, Ciência e Tecnologia do Rio Grande do SulInstituto Federal de Educação, Ciência e Tecnologia do Rio Grande do SulInstituto Federal de Educação, Ciência e Tecnologia do Rio Grande do SulUniversidade Federal da Fronteira SulUniversidade Federal da Fronteira SulUniversidade Federal da Fronteira SulAbstract Substances from the Cannabis sativa species, especially cannabidiol (CBD) and Delta-9-tetrahydrocannabinol (Δ9-THC), have attracted medical attention in recent years. The actions of these two main cannabinoids modulate the cholinergic nervous system (CholNS) involving development, synaptic plasticity, and response to endogenous and environmental damage, as a characteristic of many neurodegenerative diseases. The dynamics of these diseases are mediated by specific neurotransmitters, such as the GABAergic nervous system (GNS) and the CholNS. The nematode Caenorhabditis elegans is an important experimental model, which has different neurotransmitter systems that coordinate its behavior and has a transgene strain that encodes the human β-amyloid 1–42 peptide in body wall muscle, one of the main proteins involved in Alzheimer´s disease. Therefore, the objective of this study was to evaluate the protective potential of terpenoids found in C. sativa in the GNS and CholNS of C. elegans. The effect of two C. sativa oils with variations in CBD and THC concentrations on acetylcholinesterase (AChE) activity, lipid peroxidation, and behavior of C. elegans was evaluated. C. sativa oils were efficient in increasing pharyngeal pumping rate and reducing defecation cycle, AChE activity, and ROS levels in N2 strains. In the muscle:Abeta1-42 strain, mainly when using CBD oil, worm movement, body bends, and pharyngeal pumping were increased, with a reduced AChE activity. Consequently, greater investments in scientific research are needed, in addition to breaking the taboo on the use of the C. sativa plant as an alternative for medicinal use, especially in neurodegenerative diseases, which have already shown positive initial results.https://doi.org/10.1038/s41598-022-19598-3 |
| spellingShingle | Ana Paula Vanin Wagner Antonio Tamagno Carla Alves Letícia Mesacasa Luciani Figueiredo Santin Nathália Tafarel Sutorillo Denise Bilibio Caroline Müller Leandro Galon Rosilene Rodrigues Kaizer Neuroprotective potential of Cannabis sativa-based oils in Caenorhabditis elegans Scientific Reports |
| title | Neuroprotective potential of Cannabis sativa-based oils in Caenorhabditis elegans |
| title_full | Neuroprotective potential of Cannabis sativa-based oils in Caenorhabditis elegans |
| title_fullStr | Neuroprotective potential of Cannabis sativa-based oils in Caenorhabditis elegans |
| title_full_unstemmed | Neuroprotective potential of Cannabis sativa-based oils in Caenorhabditis elegans |
| title_short | Neuroprotective potential of Cannabis sativa-based oils in Caenorhabditis elegans |
| title_sort | neuroprotective potential of cannabis sativa based oils in caenorhabditis elegans |
| url | https://doi.org/10.1038/s41598-022-19598-3 |
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