Intra-Articular Injection of (−)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous Osteoarthritis
Osteoarthritis (OA) is the most prevalent joint disease that causes an enormous burden of disease worldwide. (−)-Epigallocatechin 3-gallate (EGCG) has been reported to reduce post-traumatic OA progression through its anti-inflammatory property. Aging is the most crucial risk factor of OA, and the ma...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/10/2/178 |
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| author | Hsuan-Ti Huang Tsung-Lin Cheng Chung-Da Yang Chi-Fen Chang Cheng-Jung Ho Shu-Chun Chuang Jhong-You Li Shih-Hao Huang Yi-Shan Lin Hsin-Yi Shen Tsung-Han Yu Lin Kang Sung-Yen Lin Chung-Hwan Chen |
| author_facet | Hsuan-Ti Huang Tsung-Lin Cheng Chung-Da Yang Chi-Fen Chang Cheng-Jung Ho Shu-Chun Chuang Jhong-You Li Shih-Hao Huang Yi-Shan Lin Hsin-Yi Shen Tsung-Han Yu Lin Kang Sung-Yen Lin Chung-Hwan Chen |
| author_sort | Hsuan-Ti Huang |
| collection | DOAJ |
| description | Osteoarthritis (OA) is the most prevalent joint disease that causes an enormous burden of disease worldwide. (−)-Epigallocatechin 3-gallate (EGCG) has been reported to reduce post-traumatic OA progression through its anti-inflammatory property. Aging is the most crucial risk factor of OA, and the majority of OA incidences are related to age and not trauma. In this study, we assess whether EGCG can ameliorate cartilage degradation in primary OA. In an in-vitro study, real-time PCR was performed to assess the expression of genes associated with human articular chondrocyte homeostasis. A spontaneously occurring OA model in guinea pigs was used to investigate the effect of EGCG in vivo. OA severity was evaluated using Safranin O staining and Osteoarthritis Research Society International (OARSI) scores, as well as by immunohistochemical (IHC) analysis to determine the protein level of type II collagen (Col II), matrix metalloproteinase 13 (MMP-13), and p16 <sup>ink4a</sup> in articular cartilage. In the in-vitro study, EGCG increased the gene expression of aggrecan and Col II and decreased the expression of interleukin-1, cyclooxygenase 2, MMP-13, alkaline phosphatase, Col X, and p16 <sup>Ink4a</sup>; EGCG treatment also attenuated the degraded cartilage with a lower OARSI score. Meanwhile, IHC results showed that EGCG exerted an anti-OA effect by reducing ECM degradation, cartilage inflammation, and cell senescence with a less-immunostained Col II, MMP-13, and p16 <sup>Ink4a</sup>. In conclusion, these findings suggest that EGCG may be a potential disease-modifying OA drug for the treatment of primary OA. |
| first_indexed | 2024-03-09T03:36:21Z |
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| id | doaj.art-4a3f9effe04f4ee599871034e2932b4c |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T03:36:21Z |
| publishDate | 2021-01-01 |
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| series | Antioxidants |
| spelling | doaj.art-4a3f9effe04f4ee599871034e2932b4c2023-12-03T14:47:59ZengMDPI AGAntioxidants2076-39212021-01-0110217810.3390/antiox10020178Intra-Articular Injection of (−)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous OsteoarthritisHsuan-Ti Huang0Tsung-Lin Cheng1Chung-Da Yang2Chi-Fen Chang3Cheng-Jung Ho4Shu-Chun Chuang5Jhong-You Li6Shih-Hao Huang7Yi-Shan Lin8Hsin-Yi Shen9Tsung-Han Yu10Lin Kang11Sung-Yen Lin12Chung-Hwan Chen13Orthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanGraduate Institute of Animal Vaccine Technology, College of Veterinary Medicine, National Pingtung University of Science and Technology, Pingtung 91201, TaiwanDepartment of Anatomy, School of Medicine, China Medical University, Taichung 40402, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanDepartment of Obstetrics and Gynecology, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan 70457, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOrthopaedic Research Center, Kaohsiung Medical University, Kaohsiung 80701, TaiwanOsteoarthritis (OA) is the most prevalent joint disease that causes an enormous burden of disease worldwide. (−)-Epigallocatechin 3-gallate (EGCG) has been reported to reduce post-traumatic OA progression through its anti-inflammatory property. Aging is the most crucial risk factor of OA, and the majority of OA incidences are related to age and not trauma. In this study, we assess whether EGCG can ameliorate cartilage degradation in primary OA. In an in-vitro study, real-time PCR was performed to assess the expression of genes associated with human articular chondrocyte homeostasis. A spontaneously occurring OA model in guinea pigs was used to investigate the effect of EGCG in vivo. OA severity was evaluated using Safranin O staining and Osteoarthritis Research Society International (OARSI) scores, as well as by immunohistochemical (IHC) analysis to determine the protein level of type II collagen (Col II), matrix metalloproteinase 13 (MMP-13), and p16 <sup>ink4a</sup> in articular cartilage. In the in-vitro study, EGCG increased the gene expression of aggrecan and Col II and decreased the expression of interleukin-1, cyclooxygenase 2, MMP-13, alkaline phosphatase, Col X, and p16 <sup>Ink4a</sup>; EGCG treatment also attenuated the degraded cartilage with a lower OARSI score. Meanwhile, IHC results showed that EGCG exerted an anti-OA effect by reducing ECM degradation, cartilage inflammation, and cell senescence with a less-immunostained Col II, MMP-13, and p16 <sup>Ink4a</sup>. In conclusion, these findings suggest that EGCG may be a potential disease-modifying OA drug for the treatment of primary OA.https://www.mdpi.com/2076-3921/10/2/178(−)-epigallocatechin 3-gallate (EGCG)cartilagecell senescenceguinea pigsosteoarthritissenescence |
| spellingShingle | Hsuan-Ti Huang Tsung-Lin Cheng Chung-Da Yang Chi-Fen Chang Cheng-Jung Ho Shu-Chun Chuang Jhong-You Li Shih-Hao Huang Yi-Shan Lin Hsin-Yi Shen Tsung-Han Yu Lin Kang Sung-Yen Lin Chung-Hwan Chen Intra-Articular Injection of (−)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous Osteoarthritis Antioxidants (−)-epigallocatechin 3-gallate (EGCG) cartilage cell senescence guinea pigs osteoarthritis senescence |
| title | Intra-Articular Injection of (−)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous Osteoarthritis |
| title_full | Intra-Articular Injection of (−)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous Osteoarthritis |
| title_fullStr | Intra-Articular Injection of (−)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous Osteoarthritis |
| title_full_unstemmed | Intra-Articular Injection of (−)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous Osteoarthritis |
| title_short | Intra-Articular Injection of (−)-Epigallocatechin 3-Gallate (EGCG) Ameliorates Cartilage Degeneration in Guinea Pigs with Spontaneous Osteoarthritis |
| title_sort | intra articular injection of epigallocatechin 3 gallate egcg ameliorates cartilage degeneration in guinea pigs with spontaneous osteoarthritis |
| topic | (−)-epigallocatechin 3-gallate (EGCG) cartilage cell senescence guinea pigs osteoarthritis senescence |
| url | https://www.mdpi.com/2076-3921/10/2/178 |
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