The diagnostic value of GICA used for intraoperative lymph node FNA-Tg measurement to evaluate thyroid cancer metastases
Objective: It is crucial to diagnose lymph node (LN) metastases (LNM) before or during thyroid carcinoma surgery. Measurement of thyroglobulin (Tg) in the fine needle aspirate washout (FNA-Tg) is useful to assist in the diagnosis of LNM for papillary thyroid carcinoma (PTC). This study aimed to asse...
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| Format: | Article |
| Language: | English |
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Bioscientifica
2024-02-01
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| Series: | European Thyroid Journal |
| Subjects: | |
| Online Access: | https://etj.bioscientifica.com/view/journals/etj/13/1/ETJ-23-0182.xml |
| _version_ | 1797336357788450816 |
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| author | Shaodong Hou Yiceng Sun Zeyu Yang Mi Tang Tingjie Yin Cong Shao Cunye Yan Linlong Mo Yuquan Yuan Supeng Yin Fan Zhang |
| author_facet | Shaodong Hou Yiceng Sun Zeyu Yang Mi Tang Tingjie Yin Cong Shao Cunye Yan Linlong Mo Yuquan Yuan Supeng Yin Fan Zhang |
| author_sort | Shaodong Hou |
| collection | DOAJ |
| description | Objective: It is crucial to diagnose lymph node (LN) metastases (LNM) before or during thyroid carcinoma surgery. Measurement of thyroglobulin (Tg) in the fine needle aspirate washout (FNA-Tg) is useful to assist in the diagnosis of LNM for papillary thyroid carcinoma (PTC). This study aimed to assess the diagnostic performance of a new technique based on a colloidal gold-based immunochromatographic assay (GICA) for intraoperative FNA-Tg in diagnosing LNM.
Clinical trial information: This study is registered with chictr.org.cn, ID: ChiCTR2200063561 (registered 11 September, 2022).
Methods: This prospective study enrolled 51 PTC patients who underwent cervical LN dissection. A total of 150 LNs dissected from the central and lateral compartments were evaluated by FNA-Tg-GICA at three different time points and compared with frozen sections and the conventional Tg measurement method electrochemiluminescence immunoassay (ECLIA). Receiver operating characteristic curve (ROC) and area under the curve (AUC), cutoff value to discriminate benign and malignant LNs, sensitivity, specificity, and accuracy were provided.
Results: The cutoff value of FNA-Tg to predict LNM was 110.83 ng/mL for ECLIA and 13.19 ng/mL, 38.69 ng/mL, and 77.17 ng/mL for GICA at 3, 10, and 15 min, respectively. There was no significant difference between the AUCs of GICA at different time points compared to using ECLIA and frozen sections. Besides, the diagnostic performance of GICA and ECLIA showed no significant difference in evaluating LNM from central and lateral compartments or between the TgAb-positive subgroup and TgAb-negative subgroup.
Conclusion: GICA is a promising method for intraoperative FNA-Tg measurement and has high value in predicting LNM. It may be a novel alternative or supplementary method to frozen section or ECLIA. |
| first_indexed | 2024-03-08T08:53:16Z |
| format | Article |
| id | doaj.art-4a4165ec223b4759ab8afc9eb5e6d08d |
| institution | Directory Open Access Journal |
| issn | 2235-0802 |
| language | English |
| last_indexed | 2024-03-08T08:53:16Z |
| publishDate | 2024-02-01 |
| publisher | Bioscientifica |
| record_format | Article |
| series | European Thyroid Journal |
| spelling | doaj.art-4a4165ec223b4759ab8afc9eb5e6d08d2024-02-01T07:49:27ZengBioscientificaEuropean Thyroid Journal2235-08022024-02-0113118https://doi.org/10.1530/ETJ-23-0182The diagnostic value of GICA used for intraoperative lymph node FNA-Tg measurement to evaluate thyroid cancer metastasesShaodong Hou0Yiceng Sun1Zeyu Yang2Mi Tang3Tingjie Yin4Cong Shao5Cunye Yan6Linlong Mo7Yuquan Yuan8Supeng Yin9Fan Zhang10Department of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, China; Clinical Medical College, North Sichuan Medical College, Nanchong, Sichuan, China Department of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, ChinaDepartment of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, ChinaDepartment of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, ChinaDepartment of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, ChinaDepartment of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, ChinaDepartment of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, ChinaDepartment of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, ChinaDepartment of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, ChinaDepartment of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, ChinaDepartment of Breast and Thyroid Surgery, Chongqing General Hospital, Chongqing, China; Clinical Medical College, North Sichuan Medical College, Nanchong, Sichuan, China Objective: It is crucial to diagnose lymph node (LN) metastases (LNM) before or during thyroid carcinoma surgery. Measurement of thyroglobulin (Tg) in the fine needle aspirate washout (FNA-Tg) is useful to assist in the diagnosis of LNM for papillary thyroid carcinoma (PTC). This study aimed to assess the diagnostic performance of a new technique based on a colloidal gold-based immunochromatographic assay (GICA) for intraoperative FNA-Tg in diagnosing LNM. Clinical trial information: This study is registered with chictr.org.cn, ID: ChiCTR2200063561 (registered 11 September, 2022). Methods: This prospective study enrolled 51 PTC patients who underwent cervical LN dissection. A total of 150 LNs dissected from the central and lateral compartments were evaluated by FNA-Tg-GICA at three different time points and compared with frozen sections and the conventional Tg measurement method electrochemiluminescence immunoassay (ECLIA). Receiver operating characteristic curve (ROC) and area under the curve (AUC), cutoff value to discriminate benign and malignant LNs, sensitivity, specificity, and accuracy were provided. Results: The cutoff value of FNA-Tg to predict LNM was 110.83 ng/mL for ECLIA and 13.19 ng/mL, 38.69 ng/mL, and 77.17 ng/mL for GICA at 3, 10, and 15 min, respectively. There was no significant difference between the AUCs of GICA at different time points compared to using ECLIA and frozen sections. Besides, the diagnostic performance of GICA and ECLIA showed no significant difference in evaluating LNM from central and lateral compartments or between the TgAb-positive subgroup and TgAb-negative subgroup. Conclusion: GICA is a promising method for intraoperative FNA-Tg measurement and has high value in predicting LNM. It may be a novel alternative or supplementary method to frozen section or ECLIA.https://etj.bioscientifica.com/view/journals/etj/13/1/ETJ-23-0182.xmlpapillary thyroid carcinomacervical lymph node metastasisfine needle aspirationthyroglobulincolloidal gold-based immunochromatographic assay |
| spellingShingle | Shaodong Hou Yiceng Sun Zeyu Yang Mi Tang Tingjie Yin Cong Shao Cunye Yan Linlong Mo Yuquan Yuan Supeng Yin Fan Zhang The diagnostic value of GICA used for intraoperative lymph node FNA-Tg measurement to evaluate thyroid cancer metastases European Thyroid Journal papillary thyroid carcinoma cervical lymph node metastasis fine needle aspiration thyroglobulin colloidal gold-based immunochromatographic assay |
| title | The diagnostic value of GICA used for intraoperative lymph node FNA-Tg measurement to evaluate thyroid cancer metastases |
| title_full | The diagnostic value of GICA used for intraoperative lymph node FNA-Tg measurement to evaluate thyroid cancer metastases |
| title_fullStr | The diagnostic value of GICA used for intraoperative lymph node FNA-Tg measurement to evaluate thyroid cancer metastases |
| title_full_unstemmed | The diagnostic value of GICA used for intraoperative lymph node FNA-Tg measurement to evaluate thyroid cancer metastases |
| title_short | The diagnostic value of GICA used for intraoperative lymph node FNA-Tg measurement to evaluate thyroid cancer metastases |
| title_sort | diagnostic value of gica used for intraoperative lymph node fna tg measurement to evaluate thyroid cancer metastases |
| topic | papillary thyroid carcinoma cervical lymph node metastasis fine needle aspiration thyroglobulin colloidal gold-based immunochromatographic assay |
| url | https://etj.bioscientifica.com/view/journals/etj/13/1/ETJ-23-0182.xml |
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