A Practical Guide to Rodent Islet Isolation and Assessment
<p>Abstract</p> <p>Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas for study is an intricate process. This review highli...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-12-01
|
| Series: | Biological Procedures Online |
| Subjects: | |
| Online Access: | http://www.biologicalproceduresonline.com/content/11/1/9021 |
| _version_ | 1818752693293809664 |
|---|---|
| author | Carter Jeffrey D Dula Stacey B Corbin Kathryn L Wu Runpei Nunemaker Craig S |
| author_facet | Carter Jeffrey D Dula Stacey B Corbin Kathryn L Wu Runpei Nunemaker Craig S |
| author_sort | Carter Jeffrey D |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas for study is an intricate process. This review highlights the key elements involved with mouse and rat islet isolation, including choices of collagenase, the collagenase digestion process, purification of islets using a density gradient, and islet culture conditions. In addition, this paper reviews commonly used techniques for assessing islet viability and function, including visual assessment, fluorescent markers of cell death, glucose-stimulated insulin secretion, and intracellular calcium measurements. A detailed protocol is also included that describes a common method for rodent islet isolation that our laboratory uses to obtain viable and functional mouse islets for in vitro study of islet function, beta-cell physiology, and in vivo rodent islet transplantation. The purpose of this review is to serve as a resource and foundation for successfully procuring and purifying high-quality islets for research purposes.</p> |
| first_indexed | 2024-12-18T04:55:31Z |
| format | Article |
| id | doaj.art-4a43c8c2389349d8ab78c3e2c32bb3ca |
| institution | Directory Open Access Journal |
| issn | 1480-9222 |
| language | English |
| last_indexed | 2024-12-18T04:55:31Z |
| publishDate | 2009-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Biological Procedures Online |
| spelling | doaj.art-4a43c8c2389349d8ab78c3e2c32bb3ca2022-12-21T21:20:17ZengBMCBiological Procedures Online1480-92222009-12-01111902110.1007/s12575-009-9021-0A Practical Guide to Rodent Islet Isolation and AssessmentCarter Jeffrey DDula Stacey BCorbin Kathryn LWu RunpeiNunemaker Craig S<p>Abstract</p> <p>Pancreatic islets of Langerhans secrete hormones that are vital to the regulation of blood glucose and are, therefore, a key focus of diabetes research. Purifying viable and functional islets from the pancreas for study is an intricate process. This review highlights the key elements involved with mouse and rat islet isolation, including choices of collagenase, the collagenase digestion process, purification of islets using a density gradient, and islet culture conditions. In addition, this paper reviews commonly used techniques for assessing islet viability and function, including visual assessment, fluorescent markers of cell death, glucose-stimulated insulin secretion, and intracellular calcium measurements. A detailed protocol is also included that describes a common method for rodent islet isolation that our laboratory uses to obtain viable and functional mouse islets for in vitro study of islet function, beta-cell physiology, and in vivo rodent islet transplantation. The purpose of this review is to serve as a resource and foundation for successfully procuring and purifying high-quality islets for research purposes.</p>http://www.biologicalproceduresonline.com/content/11/1/9021islets of Langerhanspancreatic isletsbeta-cellbeta cellscytokinestransplantationcalciuminsulinapoptosisnecrosisisolationislet isolationcollagenaserodentmouseratislet |
| spellingShingle | Carter Jeffrey D Dula Stacey B Corbin Kathryn L Wu Runpei Nunemaker Craig S A Practical Guide to Rodent Islet Isolation and Assessment Biological Procedures Online islets of Langerhans pancreatic islets beta-cell beta cells cytokines transplantation calcium insulin apoptosis necrosis isolation islet isolation collagenase rodent mouse rat islet |
| title | A Practical Guide to Rodent Islet Isolation and Assessment |
| title_full | A Practical Guide to Rodent Islet Isolation and Assessment |
| title_fullStr | A Practical Guide to Rodent Islet Isolation and Assessment |
| title_full_unstemmed | A Practical Guide to Rodent Islet Isolation and Assessment |
| title_short | A Practical Guide to Rodent Islet Isolation and Assessment |
| title_sort | practical guide to rodent islet isolation and assessment |
| topic | islets of Langerhans pancreatic islets beta-cell beta cells cytokines transplantation calcium insulin apoptosis necrosis isolation islet isolation collagenase rodent mouse rat islet |
| url | http://www.biologicalproceduresonline.com/content/11/1/9021 |
| work_keys_str_mv | AT carterjeffreyd apracticalguidetorodentisletisolationandassessment AT dulastaceyb apracticalguidetorodentisletisolationandassessment AT corbinkathrynl apracticalguidetorodentisletisolationandassessment AT wurunpei apracticalguidetorodentisletisolationandassessment AT nunemakercraigs apracticalguidetorodentisletisolationandassessment AT carterjeffreyd practicalguidetorodentisletisolationandassessment AT dulastaceyb practicalguidetorodentisletisolationandassessment AT corbinkathrynl practicalguidetorodentisletisolationandassessment AT wurunpei practicalguidetorodentisletisolationandassessment AT nunemakercraigs practicalguidetorodentisletisolationandassessment |